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Comprehensive sets of clones and improved high-throughput methods for production of functional proteins now allow proteome-scale in vitro experiments on nearly 15,000 human genes.
Efficient methods to characterize the binding properties of affinity reagents are required. A combination of bacterial surface display, flow cytometry and pyrosequencing is now used for high-speed mapping of the epitopes recognized by antibodies.
A combination of automated screening and next-generation sequencing makes it possible to identify Caenorhabditis elegans mutants at unprecedented speed and scale.
A decade after the introduction of genetically encoded Ca2+ indicator proteins (GECIs), a new generation of improved GECIs demonstrates their usefulness for the functional analysis of the mammalian brain in vivo.
Applying a classical solution to a cutting-edge problem, two groups used bacterial conjugation to construct Escherichia coli double mutants on a genome-wide scale. This will allow comprehensive genetic interaction screens in bacteria for the first time.
Algorithms for analyzing single-particle tracking images to obtain the paths of individual particles are challenged by high-density data. Improvements in algorithms help to overcome these limitations.
Two complementary approaches, both using next-generation sequencing, have successfully tackled the scale and the complexity of mammalian transcriptomes, at once revealing unprecedented detail and allowing better quantification.
Strategies for the comprehensive identification of transcript isoforms produced from specific genomic loci make use of and expand existing tools and resources.
Advances in the application of microfluidics technology to biological assays using the model organism Caenorhabditis elegans help to automate otherwise time-consuming experiments.