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On display on a bug: a systematic approach to characterize antibodies

Nature Methods volume 5, pages 1003–1004 (2008)Cite this article

An Erratum to this article was published on 01 January 2009

This article has been updated

Efficient methods to characterize the binding properties of affinity reagents are required. A combination of bacterial surface display, flow cytometry and pyrosequencing is now used for high-speed mapping of the epitopes recognized by antibodies.

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Figure 1: The epitope mapping approach.

Kim Caesar

Change history

  • 26 November 2008

    NOTE: In the version of this article initially published, Figure 1 was incorrectly described as having been adapted from Rockberry et al. The correct citation is Rockberg et al. The error has been corrected in the HTML and PDF versions of the article.

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Authors and Affiliations

  1. Thomas Knorpp and Markus F. Templin are at the Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstraße 55, 72770 Reutlingen, Germany. templin@nmi.de,

    Thomas Knorpp & Markus F Templin

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  1. Thomas Knorpp
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  2. Markus F Templin
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Knorpp, T., Templin, M. On display on a bug: a systematic approach to characterize antibodies. Nat Methods 5, 1003–1004 (2008). https://doi.org/10.1038/nmeth1208-1003

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