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Phage and robotics-assisted near-continuous evolution enables phage-assisted continuous evolution in high throughput, allowing for improved exploration of sequence space and insight into how variables affect evolution outcomes.
This Perspective offers an overview of organic dyes commonly used as fluorescent labels and gives a chemist’s insight into their benefits and peculiarities.
3D-CASH is a random-access microscopy approach that avoids in vivo motion artifacts by sampling each targeted neuron with a holographically shaped grid of illumination spots. The technology allows recording neuronal activity in the mouse cortex at a throughput of 20,000 neurons per second.
HaloTag variants offer distinct brightness and fluorescence lifetimes compared with HaloTag7 when labeled with rhodamines. These variants were used for multiplexed imaging with a single fluorophore and to create lifetime-based cell cycle indicators.
mBrainAligner is a cross-modal registration platform for whole mouse brains imaged with different modalities. In addition, a fluorescence micro-optical sectioning tomography-based mouse brain atlas has been generated.
Single-cell GET-seq (scGET-seq) enables the probing of compacted as well as accessible chromatin in single cells, covering a greater portion of the genome, which provides insights into genomic and epigenetic dynamics.