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A feasibility study for the systematic generation of affinity reagents to human proteins provides an opportunity to test the merits of recombinant affinity reagents.
A combination of automated screening and next-generation sequencing makes it possible to identify Caenorhabditis elegans mutants at unprecedented speed and scale.
The results of large genome-wide association studies (GWASs) are being deposited in public databases with increasing frequency. But the software to analyze and interpret GWAS datasets can be difficult to use. Could a new generation of user-friendly programs fill the gap?
A strategy using 48 or more singly labeled fluorescent oligonucleotide probes targeted to individual mRNA molecules allows the simultaneous localization and quantification of three mRNA species in fixed cells. mRNA visualization in whole animals and other organisms is also demonstrated.
Time-resolved wide-angle X-ray scattering (TR-WAXS) using synchrotron radiation can be used to observe dynamic protein structural changes with nanosecond time resolution in solution, complementing time-resolved optical spectroscopy and Laue crystallography methods.
Spectral searching, based on matching experimental peptide spectra to reference spectral libraries, is gaining interest as an alternative to traditional sequence-database searching in mass spectrometry–based proteomics. A software tool, SpectraST, now allows users to build their own high-quality spectral libraries from raw data.
A simple yet powerful super-resolution imaging approach based on switching off ordinary fluorophores and localizing those remaining or regaining fluorescence is illustrated using continuous widefield illumination and imaging of fixed and living cells labeled with rhodamine-derived dyes or fluorescent proteins. Biteen et al., also in this issue, describe related work using the ordinary fluorophore of EYFP for super-resolution imaging.
Previous work showed that the commonly used fluorescent protein EYFP can be bleached and reactivated. Exploiting this property allows super-resolution in vivo imaging of EYFP-labeled structures in living bacteria. Fölling et al., also in this issue, describe a related approach for super-resolution imaging using other ordinary fluorophores.
Targeted regions of the human genome are resequenced in multiplex with Illumina technology, and the pipeline is evaluated for polymorphism discovery and genotyping.
Using both behavioral and electrophysiological readouts, Channelrhodopsin-2, a light-gated cation channel, is applied to the study of synaptic function in Caenorhabditis elegans.
Researchers use tetrad analysis and high-density oligonucleotide tiling arrays to generate a high-resolution map of meiotic recombination events in budding yeast.
