Reviews & Analysis

The two-color traffic light reporter reads out what pathway is used to repair a DNA break and will increase insights into genome engineering.

An absolute quantification approach combined with differential affinity capture provides a means by which to accurately measure distinct pools of ubiquitin in cells or tissues.

Automated methods can now extract brain-image coordinates appearing in hundreds of publications in targeted topic areas and then integrate these data to form computational models that classify new brain-image data.

A technique that combines the speed of pyrosequencing with the sensitivity of fluorescent detection may lead to faster sequencing with smaller quantities of DNA.

A method for clustering billions of unidentified tandem mass spectra from shotgun proteomics experiments offers new ways of storing, organizing and analyzing proteomics data, with potential benefits to the entire proteomics community.

A large-scale, multifaceted screening and validation strategy combining traditional and recombinant antibody technologies yielded a broad spectrum of validated protein binders with high binding affinity and specificity applicable to many fields in proteome research.

Software for fast and accurate alignment of brain images is used to generate a partial brain atlas for Drosophila melanogaster and should enable circuit mapping.

A transposon-insertion method can trap and mutate genes while simultaneously revealing their spatiotemporal expression pattern, all in a conditional manner in the live, transparent zebrafish.

With vast increases in biological data generation, mechanisms for data storage and analysis have become limiting. A data structure, semantically typed data hypercubes (SDCubes), that combines hierarchical data format version 5 (HDF5) and extensible markup language (XML) file formats, now permits the flexible storage, annotation and retrieval of large and heterogenous datasets.

Improved methods for human pluripotent stem cell culture and for reprogramming of human somatic cells to pluripotency may bring us closer to the routine generation of personalized pluripotent stem cells.

A parallel microfluidic cytometer combines low-pixel-count, one-dimensional images with parallel-channel flow cytometry for high-speed, high-throughput screening of cells.

A technique to substantially increase the resolution and imaging area of Fourier-transform infrared microspectroscopy, while decreasing the amount of time required for image acquisition, may augment the use of this technology in biomedical and environmental research.

A wearable imaging device allows functional whole-brain imaging of awake, freely moving rats. This technology opens up a noninvasive window for simultaneously assessing brain function and behavior in response to a wide variety of interventions in living rats.

Pairwise quantitative genetic interactions are mapped by combinatorial RNA interference in metazoan cells.

Two fluorescence microscopy techniques provide alternative routes to obtaining three-dimensional super-resolution images with greater axial depth.