Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
Application of a single layer of graphene to untreated wet mammalian cells enables mass spectrometry imaging of cellular membranes of live cells in solution at subcellular resolution.
CryoDRGN is an unsupervised machine learning algorithm that reconstructs continuous distributions of three-dimensional density maps from heterogeneous single-particle cryo-EM data.
A multi-laboratory study in the form of a community challenge assesses the quality of models that can be produced from cryo-EM maps using different software tools, the reproducibility of models generated by different users and the performance of metrics used for model validation.
This study explores the performance of deep-learning models for super-resolution imaging and introduces models that utilize frequency content information in the Fourier domain to improve SIM reconstruction under low-SNR conditions.
This work presents a sequencing strategy based on unique molecular identifiers that improves long-read consensus sequence accuracy of targeted amplicons as well as shotgun whole-genome fragments.
The iterative Build and Retrieve (BaR) methodology facilitates the solving of cryo-EM structures of multiple membrane (and soluble) proteins simultaneously, including small and low-abundance membrane proteins.
Megabodies, built by grafting nanobodies onto larger protein scaffolds, help alleviate problems of particle size and preferential orientation at the water–air interfaces during cryo-EM based structure determination experiments and are shown to be generalizable to soluble and membrane-bound proteins.
Cell surface thermal proteome profiling allows characterization of ligand-induced changes in protein abundances and thermal stabilities at the plasma membrane.