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A rescanning microscopy approach enables two-photon image-scanning microscopy that doubles resolution relative to that of conventional two-photon microscopy at high frame rates and with high sensitivity for improved super-resolution imaging of living specimens.
A reverse two-hybrid approach combined with massively parallel programmed mutagenesis and sequencing allows profiling protein interaction determinants at amino-acid resolution, as demonstrated by identifying interaction-disrupting mutations in a cilia protein complex associated with Bardet–Biedl syndrome.
Combining each sgRNA with a unique molecular identifier in a genome-wide screen increases sensitivity and robustness in both positive and negative selection.
Enrichment of biotinylated peptides using an anti-biotin antibody results in substantially improved biotinylation site identifications by mass spectrometry compared to traditional streptavidin-based biotinylated protein enrichment.
Cryo-EM-based structure determination of macromolecular complexes at near-atomic resolution is possible using a mid-range 200-keV transmission electron microscope instrument.
The Critical Assessment of Metagenome Interpretation (CAMI) community initiative presents results from its first challenge, a rigorous benchmarking of software for metagenome assembly, binning and taxonomic profiling.
NeuBtracker is an open-source platform that enables simultaneous imaging of behavior and neural activity in freely behaving zebrafish larvae. Its performance is demonstrated during several spontaneous or stimulus-induced behaviors.
This study compares naive human pluripotent stem cells, either reprogrammed directly from somatic cells or converted from primed cells, under a variety of culture conditions.
A mass spectrometry imaging system combined with a laser triangulation system provides simultaneous topographic and molecular information for 3D biological samples and eliminates height-related artifacts in imperfect tissue sections.
A genetically encoded tension sensor module for measuring molecular forces at 3–5 pN along with tools for multiplexed tension sensing and data analysis reveal an intramolecular tension gradient across talin-1 during cell adhesion.
A tracking microscope for imaging neuronal activity at single-cell resolution in freely swimming zebrafish larvae is described. This technology allows functional imaging during behaviors that have previously been inaccessible to real-time analysis.
Blue-light-inducible CRISPR-based transcriptional activation systems achieve high enough endogenous gene expression to trigger the differentiation of iPSCs.
Guidelines for fine-tuning spectral and chemical properties of fluorophore are introduced, resulting in four new Janelia Fluor dyes, JF503, JF525, JF585 and JF635, that span the visible region and align well with standard laser lines.
Red-shifted luciferins and corresponding mutants of NanoLuc enable brighter bioluminescence imaging in vitro, in cells, and in deep tissues of living mice alone and in the context of the newly developed Antares2 BRET reporter.