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Real-time deformability cytometry allows the continuous mechanical characterization of cells with high throughput and is applied to distinguish cell-cycle phases, track differentiated cells and profile cell populations in whole blood.
Pareto task inference (ParTI) computes a polytype that encloses a data set and determines the enrichment of features around the vertices (archetypes) of the polytype, which allows the identification of the task the archetype represents.
Ribose-seq allows ribose nucleotide (rNMP) incorporation to be detected genome-wide in DNA at single-base resolution and is demonstrated on budding yeast.
A simple and general chemical structure change to a panel of cell-permeable small-molecule fluorophores increases their brightness and photostability, which will enable improved single-molecule studies and super-resolution imaging.
The combination of molecularly tagging bacteria prior to infection and high-throughput sequencing of infected sites allows the quantitative assessment of the founding population size and analysis of bacterial migration patterns.
The computational workflow of DIA-Umpire allows untargeted peptide identificationdirectly from DIA (data-independent acquisition) proteomics data without dependence on a spectral library for data extraction
Binary expression systems such as the Gal4-UAS or Q-systems are useful tools for genetic manipulation in Drosophila. Here, improved reagents for the Q-system are described.
The fluorescent proteins mEos4a and mEos4b maintain their fluorescence and photoconversion after fixation with osmium. This property enables applications such as correlative super-resolution and electron microscopy.
A comprehensive allelic series for a protein of interest with a single mutation per cDNA template can be generated using a combination of microarray-based DNA synthesis and overlap-extension mutagenesis.