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IsoView microscopy achieves rapid isotropic-resolution imaging of large, nontransparent samples using simultaneous light-sheet illumination and fluorescence detection in four orthogonal directions.
A computational pipeline for analysis and statistical evaluation of quantitative cross-linking–mass spectrometry data facilitates the investigation of protein-complex structural heterogeneity.
Fusing a DNA-binding domain to Cas9 with an attenuated, more promiscuous PAM-recognition domain increases the targeting range of Cas9 as well as its specificity.
Multiplexed hybridization probes are traditionally difficult to design with high sensitivity and specificity. Here Wu et al. present a method for fine, decoupled and on-the-fly tuning of probe behavior based on the stoichiometric formulation of a molecular competitor species.
Transparent micro-optoelectrode arrays enable simultaneous electrical recording and optical stimulation in precise alignment. Depending on the applied light levels, single-unit activity or behavioral responses can be optically evoked.
Brillouin microscopy can be used to analyze the mechanical properties of cells in a contact-free fashion. Cells in 2D and 3D environments are accessible to this technology, which provides measurements of longitudinal moduli at optical resolution.
This paper reports a Bayesian approach for the automatic identification of the optimal clustering proposal in the analysis of single-molecule localization-based super-resolution data.
The molecular architecture of protein complexes can be determined using an optimal approach for isolating GFP-tagged complexes at native levels, combined with cross-linking, mass spectrometry analysis, and structure modeling from mass spectrometry-derived distance restraints.
Single Molecule Cluster Analysis (SiMCAn) is a hierarchical clustering method that enables rapid interpretation of the complex single-molecule dynamic behaviors of cellular machines (such as the spliceosome) from single-molecule fluorescence resonance energy transfer data.
A crosslinking-mass spectrometry strategy, including a new proteome database search engine called XlinkX, enables the identification of inter- and intra-protein cross-links in cell lysates on a proteome-wide scale.
A method for profiling alterations in protein thermal stability after ligand binding using mass spectrometry identifies the cellular protein targets of drugs and metabolites in living cells.
This analysis compares 13 clustering methods on 24 data sets using the freely accessible ClustEval platform and presents guidelines for how to choose tools and set parameters for clustering biomedical data
A zinc finger–based modular DNA sequence–recognition system produces a customizable response signal that can induce apoptosis or detect virus-infected cells.
With a method involving the rapid injection of living cells into a mass spectrometer, researchers demonstrate the ability to monitor metabolite profiles in real time over the course of several hours.
Z-Brain is an atlas of the larval zebrafish brain. It can be combined with pERK-based neural-activity measurements from freely behaving zebrafish to identify brain regions involved in generating behavior.
The length of a single guide RNA (gRNA) determines the function of Cas9. In this study 20-nt gRNAs allowed nuclease activity and genome editing, whereas 14-nt gRNAs mediated transcriptional activation or repression.