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A reporter of caspase-1 activity based on Gaussia luciferase—iGLuc—reveals inflammasome activation in mouse cells and in vivo. iGLuc forms nonluminescent aggregates in cells that can be cleaved by specific proteases and, consequently, lead to luciferase activity. The design principle is applied to build reporters for several other proteases.
A systematic evaluation of parameters that affect short amplicons sequenced on Illumina platforms provides guidelines for how to eliminate erroneous reads and improve data interpretation.
Improved mass spectrometry–based detection of low-abundance proteins in complex proteomes is made possible with a method to selectively digest and deplete high-abundance proteins.
A method is reported for optical trap calibration on lipid droplets in cell extract. It should permit quantitative studies of motor proteins in a more natural context than on beads.
The eXpress software uses an online algorithm to accurately assign ambiguously mapping sequence reads for real-time read abundance estimation, thereby dramatically enhancing the efficiency of any analysis that uses large-scale sequencing.
Human fibroblasts undergo conversion to angioblast-like progenitors and then to endothelial cells by brief exposure to pluripotency factors, which is then followed by differentiation.
Open-source software that allows biologists to create a variety of behavior classifiers for automatically annotating video of behaving animals is presented. The program, called JAABA, uses state-of-the-art machine-learning methods and is applicable to tracking data from different organisms, including mice and adult and larval Drosophila.
Interactome3D is an interactive resource allowing biologists to use available structural information to model protein-protein interactions and structurally annotate protein interaction networks.