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By targeting calcium indicators to primary cilia, micrometer-long protrusions from the cellular plasma membrane, the authors measure Ca2+ signaling in these sensory organelles.
A system to control gene expression based on a destabilized form of Cre recombinase is reported. Drug-induced stabilization of Cre triggers recombination of 'floxed' alleles in the genome and is here used to genetically modify the activity of neural circuits in the mouse brain.
A statistical method that uses spike-ins to model the dependence of technical noise on transcript abundance in single-cell RNA-seq experiments allows identification of genes wherein observed variability in read counts can be reliably interpreted as a signal of biological variability as opposed to the effect of technical noise.
An algorithm and software tool, Borges, utilizes nonspecific tertiary-structure fragment information available in the Protein Data Bank to phase protein X-ray diffraction data.
This paper reports a strategy for combining somatic mutation profiles of human tumors with gene networks to stratify tumors into biologically and clinically relevant subtypes. The method is applied to ovarian, uterine and lung cancers.
A strategy, based on solid-state NMR spectroscopy, for determining the structure of an oligomeric, seven-helix membrane protein (Anabaena sensory rhodopsin) in a lipid environment is described.
This work describes wide-field temporal focusing, a two-photon volumetric imaging technique based on light sculpting that enables functional imaging of the majority of neurons in the head ganglia of C. elegans with high temporal and spatial resolution.
Addition of weak helper interactions to fluorescent protein pairs by protein engineering provides a simple method to increase FRET efficiency with little or no background.
CRISPR-Cas9–mediated cleavage is used to stimulate homologous recombination at specific target sites in the C. elegans genome, permitting flexible tagging and sequence modification of endogenous worm genes.
The addition of a low percentage of DMSO into liquid chromatography solvents strongly enhances peptide electrospray ionization, substantially improving proteome analysis by liquid chromatography–tandem mass spectrometry.
A collection of single-gene-mutant human cells is described. This growing resource is based on gene-trap mutagenesis of a near-haploid human cell line and covers almost 3,500 human genes.
A proteomic method to identify human proteins post-translationally modified by poly(ADP-ribosyl)ation is reported, which will help yield further insights into the biological role of this modification.
Renewable affinity reagents with high specificity and affinity for histone modifications perform well in ChIP-seq and other applications in epigenetics research.
To determine microbial community structure, the UPARSE software extracts operational taxonomic unit (OTU) representative sequences with high accuracy on the basis of amplified marker-gene sequences.
A combination of allele-specific and non–allele-specific probes allows in situ detection and quantification of mRNA transcripts that differ by only a few SNPs.