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Two assays reveal the birthplace of target proteins. In both, an antibody against a protein is used alongside an antibody that marks recent translation. The close proximity of both antibodies indicates the newly synthesized target protein.
HISAT (hierarchical indexing for spliced alignment of transcripts) uses global and local indices for fast, sensitive alignment with small memory requirements.
CaptureSeq was used to quantitatively profile transcripts with low expression, resulting in a catalog of long noncoding RNA expression in 20 human tissues.
ImmunoPET/CT imaging using a labeled SIV-specific antibody can identify sites of viralinfection in SIV-infected macaques without the need for tissue biopsies, even if viral load in the blood is below the detection limit.
The fusion of three transcriptional activation domains to a nuclease-deficient Cas9 achieves robust induction of gene expression and can induce differentiation of hiPSCs.
New detector technology has improved the resolution of cryo-electron microscopy (cryo-EM), but tools for structure determination from high-resolution maps have lagged behind. Wang et al. describe a de novo approach for structure determination from high-resolution cryo-EM maps. Also in this issue, DiMaio et al. report structure determination using a homologous structure as a starting model.
New detector technology has improved the resolution of cryo-electron microscopy (cryo-EM), but tools for structure determination from high-resolution maps have lagged behind. DiMaio et al. report structure determination from high-resolution cryo-EM maps using a homologous structure as a starting model. Also in this issue, Wang et al. describe a de novo approach for structure determination that does not require a starting model.
An approach to fuse images from imaging mass spectrometry and microscopy provides biological insights into molecular tissue distributions beyond what can be obtained from either modality individually.
Improved error assessment and read alignment on the MinION nanopore sequencing platform allow for calling of single-nucleotide variants and resolving the repeat structure of an assembly gap in the human X chromosome.
FIB-SEM sample size is limited by cumulative milling artifacts and long imaging times.Ultrathick sectioning, followed by parallel FIB-SEM imaging and volume stitching,overcomes this limit, generating data sets of high quality for large-scale connectomics.
In vitro digestion of genomic DNA with Cas9 and single guide RNAs (sgRNAs) yields genome-wide off-target sites at frequencies below 0.1%. Off-target sites can be further reduced with modified sgRNAs.
Two fluorescent reporters allow high-resolution visualization of auxin response; DR5v2 is more sensitive than existing auxin response reporters, and a ratiometric version of DII-Venus named R2D2 allows for accurate quantification of auxin input.