Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
Extraction conditions can have a substantial effect on protein complexes isolated from within cells. A platform for rapid, systematic screening of these conditions is described, which should enable the identification of biologically relevant complexes.
CONSERTING detects somatic copy-number alterations from whole-genome sequence data with high accuracy and sensitivity and identifies breakpoints even in heterogeneous or impure tumors.
'Spaghetti monster' fluorescent proteins combine the power of conventional fluorescent proteins with the benefits of commonly used epitopes. These probes are demonstrated to be extremely versatile in diverse imaging applications.
The topography of axonal projections can be deciphered by retrograde labeling with multiple overlapping dye injections, as the dye composition in retrogradely transported vesicles is indicative of axonal projection sites.
Kohinoor is a fast- and positively switching fluorescent protein with high photostability over many photoswitching cycles. Its improved photophysical properties enable nanoscopy with low phototoxicity and allow use of a simplified RESOLFT setup.
Metabolically labeling proteins with glycans that enable attachment of an isotopically encoded tag allows for the identification of N- and O- glycopeptides and their glycan structures.
An improved method for preparing mouse brains for electron microscopy allows reliable tracing of neurites and identification of synapses and appears suitable for whole-brain connectomic reconstruction.
Targeted enrichment and duplex sequencing yield extremely efficient, accurate and sensitive sequencing of rare subclonal mutations in small genomic target regions.
A new metric, Correlation Map (CorMap), which provides a goodness-of-fit test for comparing one-dimensional spectra without the need of estimating experimental errors, is developed and applied to evaluate small-angle X-ray scattering (SAXS) data from biological macromolecules.
Measuring the expression of a GFP-based capacity monitor in E. coli enables assessment of the burden heterologous gene expression exerts on the cell and allows for forward design of constructs with improved growth.
The biophotonic laser-assisted surgery tool (BLAST) enables the delivery of nearly any cargo type, including micrometer-sized live bacteria, into a wide variety of cell types with high efficiency, throughput and cell viability.
Photoacoustic microscopy is a hybrid imaging technology based on the conversion of absorbed light into ultrasound emission. A single-wavelength pulse-width-based method allows fast functional imaging of the brain.
Using data from global run-on and sequencing (GRO-seq) or related methods, the dREG software identifies a new class of transcriptional regulatory elements with strong evidence for directing active transcription.
The combination of deep chromatin immunoprecipitation–sequencing with a statistical test that scores the correlation of peak height and allelic imbalance allows de novo discovery of histone acetylation quantitative trait loci without prior genotyping or genome sequencing.
This study extends the natural code by which transcription activation–like effector nucleases (TALENs) recognize DNA and uses the resulting expanded repertoire of repeat divariable residues (RVDs) to improve TALEN performance.