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The genetically encoded GABA sensor iGABASnFR allows visualizing GABA signaling in vivo. Its application is demonstrated in mouse slices, in the awake mouse and in behaving zebrafish.
High-affinity sensors for free ubiquitin can be used to quantify intracellular ubiquitin pools, visualize ubiquitin levels by microscopy of fixed cells and enable real-time deubiquitination assays of diverse ubiquitin–protein conjugates.
A genetically encodable protein synthesis inhibitor (gePSI) for cell-specific inhibition of protein synthesis that is efficient and reversible enables the study of structural plasticity following single-synapse activation in neurons.
CombiSEAL is a high-throughput platform for seamlessly assembling barcoded combinatorial genetic units, offering an approach for protein optimization such as screening SpCas9 variants.
A software tool, EPIC, is developed to determine protein complex membership using chromatographic fractionation–mass spectrometry data, and is applied to map the global Caenorhabditis elegans interactome.
Conos constructs a joint graph between single cells in different samples based on multiple pairwise alignments of the samples and identifies recurrent subpopulations across all of the datasets.
The red form of the photoconvertible fluorescent protein mEos4b has a long-lived dark state with specific chromophore conformation. Weak 488-nm light depopulates this state, improving track lengths in single-particle tracking experiments.
The automated structures analysis program (ASAP) enables rapid and objective detection, classification and analysis of cellular assemblies in super-resolution images.
A microfluidic approach allows spatiotemporal control of morphogen distribution in human pluripotent stem cell cultures. This approach recapitulates early developmental processes such as localized signaling and symmetry-breaking events.
Pathway-level information extractor (PLIER) uses prior knowledge of pathways to extract biologically interpretable latent variables from large gene expression datasets.
Two-photon microscopy in combination with adaptive optics enables diffraction-limited morphological and functional imaging up to around 800 μm below the pia. This is achieved with the help of fluorescent microvessels serving as guidestars.
The ‘jGCaMP7’ sensors are four genetically encoded calcium indicators with better sensitivity than state-of-the-art GCaMP6 and specifically improved for applications such as neuropil or wide-field imaging. The sensors are validated in vivo in both flies and mice.
Tagging live single cells and nuclei with lipid- or cholesterol-modified oligonucleotides enables massive scRNA-seq sample multiplexing, identifies doublets and recovers cells with low RNA content.
FEAST provides a computationally efficient tool to estimate the contribution of microbial sources to a target microbial community, as demonstrated for a variety of complex environmental samples.
HiChIRP combines a modified chromosome conformation capture protocol with enrichment of RNA-associated chromosome conformation to visualize genome-wide looping linked to an RNA of interest.