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The multi-trait set test (mtSet) is an efficient mixed-model method that enables genome-wide association tests for large cohorts and multiple quantitative traits using sets of variants to improve power and sensitivity, while addressing confounding due to population structure.
This Analysis reports a comparison of current software packages for single-molecule localization in localization-based super-resolution imaging. Performance of the participating software on synthetic, biologically inspired ground-truth data was assessed by multiple criteria.
This paper reports an approach to measure equilibrium binding affinities for interacting proteins in high throughput, allowing the rapid and quantitative profiling of the specificity of interaction motifs.
CRISPR-Display is a modular and flexible platform that targets natural or synthetic noncoding RNA domains to specific genomic loci for functional studies and synthetic biology.
An analytical method, with accompanying software, is described for improved fidelity in traction force microscopy and is used to measure forces at emerging focal adhesions at high resolution.
A human-liver chimeric mouse supports the growth of recombinant Plasmodium falciparum progeny, enabling easy analysis of genetic crosses between parasite strains.
Bioluminescence resonance energy transfer, from the substrate of a luciferase fused with a G protein–coupled receptor to a fluorescent dye covalently linked to a receptor ligand, allows the profiling of ligand affinity and binding kinetics.
This paper shows that microfluidic perfusion frequency can be optimized to improve the differentiation of human pluripotent stem cells along different lineages, and uses this principle to achieve functional hPSC differentiation directly on a chip.
A robotic system is described that uses machine vision guidance to pick, manipulate, inspect and dissect individual flies, enabling large-scale and automated approaches to the analysis of morphology, behavior and neural dynamics.
A method for 3D differentiation of human pluripotent stem cells yields brain cortical spheroids with functional neurons and astrocytes. The spheroids can be sliced for imaging and electrophysiological studies.
Dynamic nuclear polarization–based solid-state nuclear magnetic resonance spectroscopyis applied to study the structure of a large bacterial membrane protein complex in its cellular environment.
The Cluster-Chip provides highly efficient and gentle capture of circulating tumor cell clusters from milliliters of unprocessed whole blood, making it possible to study how these clusters contribute to metastasis.
Primed conversion of proteins such as Dendra2 confines photoconversion to a limited 3Dvolume through a sequential absorption process relying on illumination with two separate low-intensity lasers and enables precise targeting of single cells.
The first report of the ICGC-TCGA DREAM Somatic Mutation Calling Challenge introduces the BAMSurgeon tool for accurate tumor simulation and reports the performance of 248 submissions in calling single-nucleotide variants from three pairs of synthetic tumor–normal genome benchmarks.
Tissues from multicellular organisms can be imaged by cryo-electron tomography using an optimized combination of vitrification, milling and application of fiducial markers.
A computational approach—including a cross-validation metric—for automated model building and refinement using X-ray fiber diffraction data is described and applied to solve structures of protein fibers.
Light-sheet microscopy using a single objective and micromirrors to orient the light sheet enables high- and super-resolution imaging of cells and embryos on a standard microscope.