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Single DNA-binding proteins can be tracked on densely covered DNA at high spatial and temporal resolution and in the presence of high protein concentrations by using a technique that combines optical tweezers, confocal fluorescence microscopy and stimulated emission depletion (STED) nanoscopy.
A combination of detection probes, targeting a single-nucleotide variant on individual transcripts, and guide probes to diminish the amount of false positives, enables quantification of allele-specific gene expression.
An algorithm and software tool to uncover contact networks of interacting conformationally heterogeneous protein residues from X-ray crystallography data is described.
Synchrotron-based Fourier transform infrared (FTIR) spectro-microtomography is a nondestructive, label-free imaging technique that allows chemical fingerprinting of intact, three-dimensional biological samples.
The specI software automatically and highly accurately delineates and assigns bacterial species based on a set of universal marker genes that it extracts from sequenced genomes.
Targeting unique variants in highly identical paralogous genes with molecular inversion probes followed by high-throughput sequencing will open a way to associate features in these duplicated genes with human traits.
Synthetic transcription factors based on the RNA-guided CRISPR-Cas9 system are used to activate endogenous genes in human cells. Also online, Gersbach and colleagues report similar developments at multiple other loci.
Synthetic transcription factors based on the RNA-guided CRISPR-Cas9 system are used to activate specific endogenous genes in human cells. Also online, Joung and colleagues report similar developments at two other loci.
An RNA aptamer specific for a protein of interest, when fused to an RNA sensor that activates a small-molecule fluorophore, can quantitate protein expression in live bacteria.
A method based on in situ sequencing by ligation enables direct reading of short segments of RNA or sequence tags in preserved tissue sections and cells.
The synthetic promoter E-SARE provides a genetic tool to tag neurons in an activity-dependent manner. The authors show the utility of this tool for labeling populations of neurons that respond to specific stimuli in living mice and for tracking the axonal projection patterns.
A growing collection of segmented and feature-extracted videos recording locomotive behavior in hundreds of C. elegans mutant strains is made available for phenotyping and further analysis.
Identifying tissue-specific expression of a selection marker and putative enhancer-driven expression of GFP with flow cytometry enriches for active enhancers.
Two incoherently superimposed orthogonal standing waves are used to create a pattern of 116,000 'doughnuts' for fast, highly parallelized coordinate-targeted super-resolution microscopy of living cells, with a large field of view.
The Contaminant Repository for Affinity Purification (CRAPome) is a database of annotated negative control-data that can be used for filtering out nonspecific interactions in affinity purification-mass spectrometry experiments.
RNA polymerase III–driven single guide RNA and a germ line promoter–driven expression of Cas9 enzyme allow heritable, targeted genome modifications in Caenorhabditis elegans.
Designed β-strand peptides stabilize integral membrane proteins for biochemical and structural studies, enabling electron microscopy analysis of the dynamic conformations of the ABC transporter MsbA.
Lipid Blast is an in silico–generated tandem mass spectral library covering more than 119,000 lipid compounds from 26 different classes, providing a useful tool for lipid identification in metabolomics studies.