News & Comment

Chromosome conformation capture data provide scaffolds for de novo genome assemblies.

Genome-wide, in vivo RNA structure probing helps reveal how RNA structure regulates gene expression.

Building an approach to quantify chinks in a protein's armor.

Individual cells of the same phenotype are commonly viewed as identical functional units of a tissue or organ. However, the deep sequencing of DNA and RNA from single cells suggests a more complex ecology of heterogeneous cell states that together produce emergent system-level function. Continuing development of high-content, real-time, multimodal single-cell measurement technologies will lead to the ultimate goal of understanding the function of an individual cell in the context of its microenvironment.

Emerging technologies are bringing single-cell genome sequencing into the mainstream; this field has already yielded insights into the genetic architecture and variability between cells that highlight the dynamic nature of the genome.

A variety of liquid-handling methods are available for labs large and small. Selecting an approach is not just a matter of budget.

Recent technical advances have enabled RNA sequencing (RNA-seq) in single cells. Exploratory studies have already led to insights into the dynamics of differentiation, cellular responses to stimulation and the stochastic nature of transcription. We are entering an era of single-cell transcriptomics that holds promise to substantially impact biology and medicine.

Single-cell genome and transcriptome sequencing methods are generating a fresh wave of biological insights into development, cancer and neuroscience. Kelly Rae Chi reports.

Long-read sequencing uncovers transcript features missed by short-read methods.

An imaging strategy based on telecommunications technology dramatically accelerates fluorescence microscopy.

A genetically encoded sensor of ATP:ADP ratio reveals the energy status of living mammalian cells.

Researchers define conditions in which human stem cells can be maintained in the ground state of pluripotency.