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By coupling multiplex iterative indirect immunofluorescence imaging with computer vision methods, researchers can detect at least 40 different proteins with subcellular resolution.
As microscopy methods for studying biology in living samples advance and demand for them grows, assessment of light damage caused by imaging becomes increasingly important.
By incorporating an image-classification task into an online video game, the Human Protein Atlas project unlocks the gaming community as a citizen science force.
The combination of single-particle electron microscopy and mass spectrometry shows potential for surveys of both the structure and the identity of protein complexes in the cell.