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Swept, confocally aligned planar excitation imaging is a fast light-sheet microscopy technique that can be applied to live samples such as behaving animals.
Blinking and photobleaching of fluorophores cause challenges in a whole range of imaging experiments. Here are some ways researchers are approaching fluorophore photostability.
The reliability and reproducibility of science are under scrutiny. However, a major cause of this lack of repeatability is not being considered: the wide sample-to-sample variability in the P value. We explain why P is fickle to discourage the ill-informed practice of interpreting analyses based predominantly on this statistic.