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A nonleaky and efficient DiCre-based conditional knockout system enables the study of essential genes in Toxoplasma gondii, revealing that the actin-myosin motor is not required for host invasion by the parasite.
Tagging of single transcripts with two fluorescent markers can be used to study many aspects of gene expression, including intrinsic noise in transcription or polymerase dynamics at a single gene.
The 3′ region extraction and deep sequencing (3′READS) method accurately identifies cleavage and polyadenylation sites, avoiding common artifacts and detecting sites in A-rich contexts. It was used to greatly expand the number of characterized sites in the mouse genome, including those in long noncoding RNAs.
An algorithm to predict protein-protein interactions using structural information yields results comparable in accuracy to those of high-throughput experiments.
Animations of biological processes are superb tools for science outreach and communication and can be useful in research too. But we need better ways to tell which parts of an animation are based on data.