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Parallel, genome-wide methylation and transcriptional profiling from a single cell (scM&T-seq) enables the discovery of associations between transcriptional and epigenetic variation.
The free EMOPEC web tool enables precise tuning of bacterial protein expression on the basis of small changes to the Shine-Dalgarno sequence; oligos can be generated to engineer expression of any Escherichia coli gene.
Stable integration of genes that facilitate the incorporation of unnatural amino acids into the amber stop codon in genes of interest allows targeted integration of acetyl-lysine into histone H3.3 and the investigation of its effects in mouse embryonic stem cells.