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Binary expression systems such as the Gal4-UAS or Q-systems are useful tools for genetic manipulation in Drosophila. Here, improved reagents for the Q-system are described.
The fluorescent proteins mEos4a and mEos4b maintain their fluorescence and photoconversion after fixation with osmium. This property enables applications such as correlative super-resolution and electron microscopy.
A comprehensive allelic series for a protein of interest with a single mutation per cDNA template can be generated using a combination of microarray-based DNA synthesis and overlap-extension mutagenesis.