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Single-molecule fluorescence experiments with microsecond time resolution are made possible using a photoprotection cocktail that reduces dye blinking and bleaching with a combination of dissolved oxygen, a triplet quencher and a free-radical scavenger.
By individually replacing 16 yeast genes encoding ABC transporters by GFP, mating and selecting for strains with accumulated mutations the authors create a Green Monster, a strain with deletions in all 16 genes.