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An efficient system for the reversion and modification of mouse gene trap alleles is presented. It is applicable to available collections of gene trap embryonic stem cell lines.
Selected reaction monitoring (SRM) is a powerful mass spectrometry technology to reliably detect selected protein targets, even those at very low abundance, but requires tedious assay development for each protein of interest. High-throughput SRM assay development is now possible by using crude synthetic peptide libraries without purification to represent each protein target.
A polarity-sensitive annexin-based biosensor called pSIVA becomes strongly fluorescent only after reversibly binding to the plasma membrane. pSIVA allows live-cell imaging of the apoptotic process in degenerating neurons in vitro and in vivo.
Researchers observe that cells of the post-implantation mouse epiblast can revert to an embryonic stem cell–like state without the addition of exogenous genes.
Neuroscience methods are undergoing a dramatic change owing to improvements in optical probes, but standardized evaluation procedures would aid probe development and uptake.
Two methods enable the drawing of genome-wide chromatin interaction maps: one looks at protein-independent folding principles, the other at protein-mediated functional interactions.