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Rapid and accurate identification of protein phosphorylation sites is achieved by a peptide library screen combining the advantages of chemistry in solution and analysis on solid support.
The newly introduced Agilent 5100 Automated Lab-on-a-Chip Platform (ALP) offers a high-throughput system to overcome the limitations of slab gel analysis in protein and DNA sizing and quantitation. Agilent, well established in the classical analytical business providing gas and liquid chromatography as well as mass spectrometry instrumentation, has now moved further into the life sciences with its microarray and microfluidic 'Lab-on-a-Chip' solutions.
Southern transfer and hybridization1 is used to study how genes are organized within genomes by mapping restriction sites in and around segments of genomic DNA. This protocol describes the first stages of Southern blotting: digestion of genomic DNA with restriction enzymes, separation of the resulting fragments by gel electrophoresis, and capillary transfer of the denatured fragments to a membrane2.