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A concentric-flow microfluidic electrokinetic sample injector enables efficient delivery of microcrystals in their mother liquor for serial femtosecond X-ray crystallography with minimal sample consumption.
Addition of a glycan precursor to cells results in the synthesis and secretion of a large variety of O-glycans that can be purified and biochemically analyzed to profile the cellular O-glycome.
NeuroGPS-Tree can reconstruct individual neurons from dense populations of fluorescently labeled neurons using computational strategies inspired by the strategies humans use.
Pooling barcoded 3C libraries and simultaneously capturing interactions at many loci of interest generates reproducible cis- and trans-interaction maps at high resolution from low amounts of input material. This allows for the comparison of interactions in different cell types using common software designed for differential analysis of sequence count data, rather than requiring software specifically designed for 3C experiments.
Parmbsc1, a new force field for DNA simulations, was broadly tested on nearly 100 DNA systems and overcame simulation artifacts that affected previous force fields.
The Fixed and Recovered Intact Single-cell RNA (FRISCR) method enables robust RNA extraction and sequencing from fixed, stained and sorted single cells and allows unprecedented profiling of rare cell types, including two subpopulations of radial glial cells in the developing human cortex.
Combining a reversibly photoswitchable bacteriophytochrome with photoacoustic imaging allows for exceptionally sensitive tomographic imaging deep in living mice, as well as super-resolution photoacoustic microscopy.
Handling and quantitative image analysis of layered tissues is greatly simplified by cartography with the Image Surface Analysis Environment (ImSAnE), as demonstrated on a variety of specimens, including a beating heart.
A fusion of RNA-binding proteins (RBPs) to a poly(U) polymerase allows the tagging of endogenous RNAs bound by the RBPs with a U-tail that can be used to identify the bound RNA by sequencing. RNA tagging is suited to discover RNA-protein networks in vivo.
A method for profiling changes in membrane protein thermal stability upon ligand binding using mass spectrometry identifies cellular membrane protein targets of small molecules.