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A method for phase contrast imaging of unstained thick tissue samples is presented. It is based on oblique back-illumination and can image at depths of several tens of microns.
The resolution achievable with single-molecule–based super-resolution fluorescence imaging is increased via a fluorophore caging procedure that uses a reducing agent to convert dyes to a long-lived dark state from which they can be activated with UV light and emit high numbers of photons.
Silica-coated silver nanocube suspensions provide an easy, rapid and label-free way to quantify protein binding to supported lipid bilayers by localized plasmon resonance measurement with a standard laboratory UV spectrophotometer.
A method for staining and embedding the entire mouse brain for electron microscopy is reported. The method results in uniform myelin staining and will allow reconstructions of myelinated long-range axons using serial block-face electron microscopy.
Modifications to an Orbitrap-based mass spectrometer enable analysis of large protein complexes in native-like states by mass spectrometry with very high sensitivity and mass resolution.
Cells are dosed with magnetic nanoparticles and patterned onto micromagnetic substrates, enabling the application of controlled and variable mechanical force to tens of thousands of cells.
The probe selection for imputation (PSI) approach accurately imputes global gene expression profiles from a small subset of probes that it chooses based on a training set of full profiles, allowing many more combinatorial experiments to be performed given the same resources.
A unique multiple cloning site (MCS) and defined genetic components without MCS restriction sites allow for the rapid construction and iterative tuning of synthetic genetic circuits.
The Strand-seq method independently sequences each parental strand of template DNA from single proliferating cells. It can be used to detect sister chromatid exchange and other chromosomal abnormalities at high resolution and to correct contig misorientations in genome assemblies, with potential for strand-inheritance and haplotyping studies.
A synthetic peptide library in conjunction with liquid chromatography–tandem mass spectrometry identifies the specificities of endo- and exopeptidases without requiring enrichment of substrates or products.
A genetic tool that converts bacterial regulators of translational initiation into regulators of transcriptional elongation is described. This adaptor is used to engineer several transcriptional attenuators and activators that can be predictably assembled into higher-order gene regulatory functions.
Parallel reaction monitoring (PRM)-based targeted mass spectrometry is comparable in performance to selected reaction monitoring (SRM) but requires much less investment in assay development for targeted proteomics applications.