Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
Methods are reported for the combination of fluorescence nanoscopy using either stimulated emission depletion microscopy (STED) or photoactivated localization microscopy (PALM) with electron microscopy, to achieve correlative imaging in which the super-resolved fluorescence signal is placed in the context of cellular ultrastructure.
Tracking the displacement of fluorescent beads surrounding a cell embedded in a hydrogel matrix allows quantitative measurement of the three-dimensional traction forces exerted by the cell.
A soft X-ray microscope design using partially incoherent light and a sample holder that can be tilted permits three-dimensional ultrastructural imaging of cryopreserved adherent mammalian cells without chemical fixation.
Defined surfaces displaying heparin-binding peptides support long-term culture of multiple human embryonic and induced pluripotent stem cell lines in defined media.
A statistical approach to quantitatively derive single and double mutant fitness from colony-based growth assays is described. The resultant SGA score permits assessment of yeast fitness and genetic interactions on the genome scale.
The mixture of isoforms model (MISO) assesses the confidence in estimates of the abundance of spliced exons or isoforms from paired-end RNA-seq data and detects their differential expression.
High-throughput sequencing of RNA fragments generated from a single-strand RNA-specific nuclease followed by novel computational analysis yields structural insights into noncoding RNA at the transcriptome level.
Protein modules that dimerize rapidly upon exposure to light are reported. They permit light-induced control of dimerization of fused protein targets and can be manipulated with two-photon illumination for experiments in thick samples and in vivo.
Readily synthesized maltose–neopentyl glycol (MNG) amphiphiles are useful reagents for stabilizing, extracting and crystallizing a variety of integral membrane proteins and have favorable properties relative to conventional detergents.
The community of scientists should celebrate the Nobel Prize, even if awards bestowed on one discipline are associated with another discipline. A new prize might help.