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Protein dynamics can be studied in single living cells by time-resolved fluorescent imaging of the unfolding of a fluorescence resonance energy transfer (FRET) probe—labeled protein as fast temperature jumps are applied.
A Rosetta full-atom framework, called fragment assembly of RNA with full-atom refinement (FARFAR), allows the de novo structure prediction and design of noncanonical RNA motifs with near-atomic resolution.
Chemically inducible dimerization probes selectively target proteins to the surface of specific organelles or tether organelles to each other, thus allowing precise spatiotemporal analysis of signaling events.
By subdividing a charge-coupled device (CCD) array into subgroups using a digital micromirror device and offsetting exposure times, temporal pixel multiplexing allows simultaneous high-speed and high-resolution imaging using a single CCD. This imaging modality allows 250 Hz microscopic imaging of fast cellular responses with a 10-Hz 1.3 megapixel camera
Simple minicircle vectors carrying four reprogramming factors induce pluripotency in adult human adipose stem cells and in neonatal fibroblasts without integration into the genome.
The fates of cultured neural progenitor cells can be predicted by algorithmic information theory-based computational analysis of time-lapse images of the cells.
A Cre-loxP–based technique allows triggering of heritable coexpression of a fluorescent marker along with any desired transgene, providing a versatile tool for clonal analysis of gene function in the zebrafish.
The use of membrane-tethered toxins to selectively block ion channel function in vivo is demonstrated. The approach is applied to blockade of voltage-gated calcium channels for inhibition of neurotransmission in the mouse.