Abstract
Cryptosporidium infects gastrointestinal epithelium and is a leading cause of infectious diarrhea and diarrheal-related death in children worldwide. There are no vaccines and no fully effective therapy available for the infection. Type II and III interferon (IFN) responses are important determinants of susceptibility to infection but the role for type I IFN response remains obscure. Cryptosporidium parvum virus 1 (CSpV1) is a double-stranded RNA (dsRNA) virus harbored by Cryptosporidium spp. Here we show that intestinal epithelial conditional Ifnar1−/− mice (deficient in type I IFN receptor) are resistant to C. parvum infection. CSpV1-dsRNAs are delivered into host cells and trigger type I IFN response in infected cells. Whereas C. parvum infection attenuates epithelial response to IFN-γ, loss of type I IFN signaling or inhibition of CSpV1-dsRNA delivery can restore IFN-γ-mediated protective response. Our findings demonstrate that type I IFN signaling in intestinal epithelial cells is detrimental to intestinal anti-C. parvum defense and Cryptosporidium uses CSpV1 to activate type I IFN signaling to evade epithelial antiparasitic response.
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Introduction
Cryptosporidium, an obligate intracellular apicomplexan parasite that infects mammalian gastrointestinal epithelium, is one of the leading pathogens responsible for moderate-to-severe diarrhea in children, particularly under the age of two, and an important contributor to early childhood mortality1,2. Cryptosporidium is also an important opportunistic pathogen of immunocompromised patients and those undergoing immunosuppressive therapy3. There are no vaccines and no fully effective therapy available for the infection. Current understanding of Cryptosporidium biology and molecular mechanisms of parasite-host interactions is still obscure, hampering the development of effective therapeutic strategies4,5,6
Humans are infected by ingesting Cryptosporidium oocysts, mainly the C. parvum and C. hominis species3. After excystation in the gastrointestinal tract to release infective sporozoites, each sporozoite then attaches to the membrane at their tip of intestinal epithelial cells (mainly enterocytes) and forms an intracellular but extra-cytoplasmic parasitophorous vacuole at the brush border3,5. Within the intestine, the infection is limited to epithelial cells and thus, intestinal epithelial cells provide the first line of defense and play a critical role in the initiation, regulation, and resolution of both innate and adaptive immune reactions6.
The interferon (IFN) family can generally be classified into three main types: type I (e.g., IFN-α and IFN-β), type II (IFN-γ), and type III (IFN-λ family)7. Type I IFNs bind to the conserved receptor IFNAR1/2 to induce transcription of type I IFN-stimulated genes8. Although type III IFNs bind to the IFNLR1/IL-10RB receptor, they induce highly similar transcriptional responses as type I IFNs7. While originally considered to be redundant to type I IFNs, type III IFNs have now been shown to play a unique role in protecting mucosal surfaces against pathogen challenges9. Intestinal epithelial cells can produce various type I and III IFNs and act on themselves in an autocrine manner9. IFN-γ binds to the heterodimeric receptor of IFNGR1 and IFNGR2 to induce transcription of IFN-γ-stimulated genes7.
Intestinal epithelial cells themselves do not produce IFN-γ but it is well established that IFN-γ produced from immune cells residing within the epithelium is essential to intestinal innate defense to control Cryptosporidium infection10,11. Recent studies demonstrated that Cryptosporidium can induce a profound type III IFN response in intestinal epithelial cells which is required for early intestinal anti-cryptosporidial defense12,13. Cryptosporidium infection also induces type I IFN response in the intestinal epithelium14,15,16 but there remains controversy about the role for type I IFNs in intestinal anti-Cryptosporidium defense. Induced levels of various type I IFN, such as IFN-β1 and IFN-α, are usually very mild in cultured intestinal epithelial cells following Cryptosporidium infection13. Although pre-treatment of epithelial cells with IFN-α before exposure to C. parvum could modestly inhibit parasite invasion of host cells in vitro14, type I IFN signaling does not protect C. parvum infection in IFN-γ knockout (KO) immunocompromised mice17. A recent study by Gibson et al. demonstrated that BL6 mice lacking the type I IFN receptor, Ifnar1−/−, consistently resulted in a significantly reduced parasite shedding following C. parvum oocysts oral administration when compared to wild-type mice12, providing strong evidence that type I IFN signaling functions differently from type II/III IFN signaling in host anti-Cryptosporidium defense. In addition, to counteract IFN-γ-mediated defense responses, C. parvum has evolved strategies to dysregulate IFN-γ signaling in infected host cells5,18,19 and the underlying mechanism remains obscure.
Cryptosporidium parvum virus 1 (CSpV1), a member of the family Partitiviridae, genus Cryspovirus, is a double-stranded RNA (dsRNA) virus of C. parvum and many other Cryptosporidium spp20. CSpV1 virus is non-enveloped with a genome comprised of two distinct dsRNAs, designated as CSpV1-dsRdRp (1836 bp) and CSpV1-dsCA (1510 bp)21. CSpV1-dsRNAs are not 5’-capped and unlikely to be 3’-polyadenylylated20,22. Herein, we report that conditional intestinal epithelial Ifnar1 KO neonatal mice are resistant to C. parvum infection. Moreover, C. parvum infection triggers type I IFN response in intestinal epithelium and attenuates IFN-γ-mediated intestinal epithelial antiparasitic defense through delivery of CSpV1-dsRNAs. Our data indicate a detrimental effect of type I IFN signaling in intestinal epithelial cells on intestinal anti-C. parvum defense and reveal an important strategy of immune evasion by the parasite.
Results
Resistance to C. parvum infection in vivo in conditional intestinal epithelial Ifnar1 KO mice
We cross-bred the Ifnar1fl/fl mice [B6(Cg)-Ifnar1tm1.1Ees/J, Jackson Lab] with the Villin.Cre transgenic [B6.Cg-Tg(Vil1-cre)1000Gum/J, Jackson Lab] and generated conditional intestinal epithelial Ifnar1 KO mice (Villin.Ifnar1−/− mice) (Supplementary Fig. 1a, b). Villin.Ifnar1−/− mice grow normally and the Villin.Ifnar1−/− neonates showed similar ileal morphological features as the Ifnar1fl/fl littermates, including HE staining (Fig. 1a), epithelial expression of the epithelial cellular adhesion molecule (EpCAM) and villin, and the presence of proliferating cells (PCNA-positive) and lysozyme-positive Paneth cells in the crypts by immunostaining (Supplementary Fig. 1b, c).
Neonates (5-day old, male and female animals were randomly used) of the cross-bred mice were infected by oral administration of C. parvum oocysts23,24. Ileal tissues (4 cm of small intestine from the ileocecal junction) were collected at 48 h and 72 h post infection (p.i.) either for morphologic observation or for isolation of ileal epithelium. Parasite burden was measured using real-time quantitative PCR (RT-qPCR) with primers specific for cp18S (Fig. 1b) and cpHsp70 (Supplementary Fig. 1d) and by counting after immunofluorescent staining (Fig. 1b, c)25,26. A significantly lower infection burden was detected in the ilea from the Villin.Ifnar1−/− mice than the Ifnar1fl/fl control (Fig. 1a-c and Supplementary Fig. 1b–d). A decrease in ileal villus heights, accompanied with increased cell proliferation (PCNA-positive) and expansion of the crypt regions, was observed in both infected Villin.Ifnar1−/− and Ifnar1fl/fl mice compared with non-infected animals (Fig. 1c). Epithelial labeling of EpCAM (Fig. 1c) and villin, and crypt distribution of Paneth cells (Supplementary Fig. 1c) were similar in infected animals of both genotypes.
We then measured C. parvum infection burden in cultured IEC4.1 cells (transformed but non-tumorigenic intestinal epithelial cells from neonatal mice)27 and stable IEC4.1 cells deficient in Ifnar1 (IEC4.1-Ifnar1−/− cells) (Supplementary Fig. 2). Parasite burden at 24 h after exposure to C. parvum (a time point reflecting activating of the cellular defenses protecting against infection)16, as well as at 2 h after exposure to parasite (a time point reflecting the initial attachment and invasion of the parasite into host cells)16,28, was similar between IEC4.1 and IEC4.1-Ifnar1−/− cells (Fig. 1d). Thus, conditional intestinal epithelial Ifnar1−/− mice develop resistance to C. parvum intestinal infection (Fig. 1b). However, in vitro infection employing Ifnar1 KO intestinal epithelial cells showed no changes in C. parvum attachment/invasion or epithelial cell antiparasitic defense.
Alterations in gene expression profiles, including various types of IFNs and IFN-stimulated genes, in the intestinal epithelium or cell cultures following C. parvum infection
We first measured the expression levels of selected type I, II, and III IFN genes in the neonatal intestinal epithelium and in IEC4.1 cells following infection. The RNA levels of several type I IFNs (Ifna13, Ifna7, and Ifnb1), as well as type II Ifng and type III Ifnl2/3, were significantly increased in the intestinal epithelium at 48 h p.i. (Fig. 2a). The RNA levels of many type I IFN genes, including Ifna13, Ifna7, Ifna1/5/6, Ifna1/2/5, Ifna4, Ifnab, Ifna12, and Ifnb1, were significantly increased in IEC4.1 cells 24 h post-infection (Fig. 2b). However, Ifng RNA was not detected in either infected or non-infected IEC4.1 cells and no significant increase of Ifnl2/3 at the RNA level was detected in IEC4.1 cells at 24 h after infection (Fig. 2b). The protein level of IFN-β1 was measured and confirmed to be increased in the supernatants of infected IEC4.1 cultures (24 h and 48 h p.i.) (Fig. 2b). The protein levels of IFN-γ and IFN-λ2/3 in non-infected and infected IEC4.1 cells were both below the detectable limit of the ELISA kit (DY485 and DY1789D from the R & D Systems).
We performed the RNA-Seq analysis of the ileal epithelium from infected Villin.Ifnar1−/− and Ifnar1fl/fl mice (48 h and 72 h p.i.). The complete list of genes differentially expressed between uninfected and infected Ifnar1fl/fl or Villin.Ifnar1−/− mice were listed in Supplementary Data 1. For the Ifnar1fl/fl mice, a total of 2120 and 3,665 protein-coding genes showed a significantly different expression level (adjusted p < 0.05 with fold change either >1.5 upregulated or <0.5 downregulated) between uninfected and infected neonates at 48 h and 72 h p.i., respectively (Supplementary Data 1). For the Villin.Ifnar1−/− mice, expression levels of 592 and 1142 genes were significantly different (p < 0.05) between uninfected and infected animals (at 48 h and 72 h p.i., respectively) (Supplementary Data 1). Comparison of differentially expressed genes between the two infected genotypes revealed that a majority of genes whose expression levels were altered in infected Ifnar1fl/fl mice (78.25% and 71.54% of total genes with an altered expression level at 48 h and 72 h p.i., respectively) showed no changes in Villin.Ifnar1−/− neonates at the same timepoint after infection (Fig. 2c), suggesting a strong type I IFN-associated gene transcription in the intestinal epithelium of the wild-type Ifnar1fl/fl neonates following infection. Gene set enrichment analysis (GSEA) of the gene expression profile in the infected Ifnar1fl/fl mice revealed various biological pathways associated with infection, including upregulation of genes for the type I IFN production and cellular response to type I IFNs, IFN-γ-mediated signaling and cytokine-mediated signaling pathways (Fig. 2d), consistent with findings from previous studies13,15,29. Induction of the type I/III IFN-stimulated genes was generally suppressed in the ilea from infected Villin.Ifnar1−/− mice (Supplementary Data 1), particularly at 48 h p.i. (Fig. 2d–f). Specifically, GSEA revealed a significant decrease in cellular response to type I IFNs in the infected Villin.Ifnar1−/− mice compared with the infected wild-type Ifnar1fl/fl neonates. In contrast, the expression levels of many IFN-γ-stimulated genes were comparable between infected Villin.Ifnar1−/− and Ifnar1fl/fl animals (Fig. 2d–f). Interestingly, upregulation of 18 genes, including Adora2b, Mttp, Arhgap26, Btnl6, Erbb2, Lipe, and Vmp1, was further significantly enhanced in the infected ilea of Villin.Ifnar1−/− mice (48 h p.i.), compared with infected Ifnar1fl/fl littermates (Fig. 2e, f). This panel of genes were represented as the “miscellaneous (misc.) upregulated genes”. Nevertheless, the expression levels of most other inflammatory genes or genes that code proteins for cell proliferation and apoptosis were comparable between infected Villin.Ifnar1−/− and Ifnar1fl/fl animals (Fig. 2d and Supplementary Data 1), which may account for the similar morphological features and intestinal damage observed between infected Villin.Ifnar1−/− and Ifnar1fl/fl groups (Fig. 1a, c).
RNA-Seq analysis revealed significant alterations in gene expression levels in IEC4.1 and IEC4.1-Ifnar1−/− cells at 24 h p.i. (Supplementary Fig. 3a and Supplementary Data 2). GSEA revealed several biological pathways associated with infection in infected IEC4.1 cells, including increase of type I IFN production and cellular response to type I IFNs and cytokine-mediated signaling pathway (Supplementary Fig. 3b). However, no significant increase of signaling associated with cellular response to IFN-γ was observed in infected IEC4.1 cells (Supplementary Fig. 3b). Comparison of differentially expressed genes between infected IEC4.1 and IEC4.1-Ifnar1−/− cells show that a majority of genes whose expression levels were altered in infected IEC4.1 cells (73.01% of total genes with an altered expression level at 24 h p.i.) showed no changes in the infected IEC4.1-Ifnar1−/− cells (Fig. 2g), suggesting a strong type I IFN-associated gene transcription in IEC4.1 cells following infection in vitro. Indeed, GSEA revealed that signaling pathways associated with cellular response to type I IFNs was blocked in infected IEC4.1-Ifnar1−/− cells (Fig. 2h). Upregulation of type I/III IFN-stimulated genes induced by infection was generally suppressed in the infected IEC4.1-Ifnar1−/− cells (Supplementary Fig. 3c). In addition, no significant induction of these “misc. upregulated genes” was observed in IEC4.1 and IEC4.1-Ifnar1−/− cells following infection (Supplementary Fig. 3c). Expression levels of selected genes associated with various types of IFNs in in vitro infected cell cultures or intestinal epithelium from in vivo C. parvum infection were further validated at the RNA level using RT-qPCR (for Ifnb1, Ifi44, Iigp1, Ifng, Ido1, Ifn1/2/5, Oas1g, and Usp18; Supplementary Fig. 3d, e) and at the protein level by Western blot (for Isg15 and Usp18, Supplementary Fig. 3f). The above data demonstrate differential gene expression profiles between infected intestinal epithelium from Villin.Ifnar1−/− and Ifnar1fl/fl neonates or infected IEC4.1 and IEC4.1-Ifnar1−/− cells, suggesting a strong type I IFN response in intestinal epithelial cells following Cryptosporidium infection.
Delivery of CSpV1-dsRNAs into infected host cells following C. parvum infection
Molecules of parasite origin can selectively be delivered into host cells following C. parvum infection30,31,32. The genome of CSpV1 comprises two distinct dsRNAs, CSpV1-dsRdRp and CSpV1-dsCA (Supplementary Fig. 4a). Using RT-qPCR, we detected a high level of CSpV1-dsRdRp and CSpV1-dsCA in the cytoplasmic fraction of infected IEC4.1 cells (Fig. 3a). In contrast, several parasite RNA transcripts abundantly expressed in C. parvum during the early infection stage33,34 were not detected in the cytoplasmic fraction from infected cells (Fig. 3a). Using in situ hybridization, we confirmed the cytoplasmic presence of CSpV1-dsRdRp in infected IEC4.1 cells (Fig. 3b). Notably, neither CSpV1-dsRdRp nor CSpV1-dsCA was detected in cells after exposure to heat-killed parasites (Supplementary Fig. 4b), indicating that CSpV1-dsRNA delivery is not due to nonspecific endocytosis.
J2 is a monoclonal antibody developed to recognize global viral dsRNAs35. Using the J2 antibody for immunofluorescent staining, we observed positive signal in the cytoplasm of infected IEC4.1 cells or cells after transfection of CSpV1-dsRNAs (Fig. 3c). Such positive labeling was not observed in the uninfected cells or cells transfected with a non-specific scrambled double-stranded siRNA (Control RNA) (Fig. 3c). Dot blotting also detected in vitro-transcribed CSpV1-dsRNAs (Fig. 3d) and revealed the presence of viral dsRNAs in the lysate of C. parvum sporozoites (Fig. 3e) and the cytoplasmic fraction of infected IEC4.1 cultures or cells transfected with CSpV1-dsRNAs (Fig. 3f). An RNA immunoprecipitation (RIP) assay was performed using the J2 antibody and the presence of CSpV1-dsRdRp and CSpV1-dsCA was detected in the immunoprecipitates from infected cells or cells transfected with in vitro transcribed CSpV1-dsRdRp or CSpV1-dsCA (Fig. 3g). Selected RNAs of IEC4.1 cells origin (Gapdh and Actin) and parasite origin (Cgd7_FLc_0990 and Cgd7_FLc-1000)32 were not detected in the immunoprecipitates using J2 (Supplementary Fig. 4c), confirming the specificity of J2 antibody.
Host delivery of CSpV1-dsRNAs triggers activation of type I IFN signaling in infected cells
We first examined type I IFN response in IEC4.1 cells following transfection with in vitro transcribed CSpV1-dsCA or CSpV1-dsRdRp alone. Upregulation of type I IFN and type I IFN-stimulated genes was observed in cells transfected with either CSpV1-dsRdRp or CSpV1-dsCA alone (Fig. 4a). Transfection of the same amount of single-stranded RNA of CSpV1-RdRp or CSpV1-CA (either the forward or reverse) caused only a minor induction of type I IFN-stimulated genes (Fig. 4b), less than one-tenth of the levels compared with CSpV1-dsRdRp or CSpV1-dsCA.
We then performed RNA-Seq analysis in IEC4.1 cells after transfection of CSpV1-dsRdRp, CSpV1-dsCA, or in combination for 24 h and compared their gene expression profiles with that in infected IEC4.1 cells (24 h p.i.). Significant alterations in the gene expression profiles were observed in cells transfected with CSpV1-dsRdRp, CSpV1-dsCA, or together, compared with cells transfected with a non-specific scrambled double-stranded siRNA (Control RNA) (Fig. 4c, d). A comparison of genome-wide transcriptomics indicated that genes whose expression levels altered in cells transfected with either CSpV1-dsRdRp, or CSpV1-dsCA, or together overlaid partially with that in cells following C. parvum infection (Fig. 4c, d). Specifically, similar to cells following infection (24 h p.i.), expression levels of many type I IFN-stimulated genes were increased in cells after transfection of either CSpV1-dsRdRp or CSpV1-dsCA alone or in combination for 24 h (Fig. 4e). A complete list of genes differentially expressed in cells transfected with Control RNA, CSpV1-dsRdRp, CSpV1-dsCA, or together was listed in Supplementary Data 3. Moreover, we developed an approach to interfere with CSpV1-RNA delivery through transfection of host cells with the siRNA combinations (siPOOLs) designed to target CSpV1-dsRNAs, because transfection of C. parvum parasite with any plasmid remains a technical challenge if not impossible3,36. Compared with infected cells treated with the siRNA Control (siControl), IEC4.1 cells transfected with siPOOLs to CSpV1-dsRNAs following infection showed a decreased level of CSpV1-RdRp level (Fig. 4f) with a similar infection burden (Fig. 4g) but a significant inhibition on C. parvum-induced Ifnb1 expression (Fig. 4h).
CSpV1-dsRNAs induce type I IFN response in infected cells through activating the Pkr and Rig-I/Mavs/Sting signal pathways
Viral dsRNAs are recognized by intracellular dsRNA sensors to trigger type I IFN gene transcription, such as the interferon-inducible protein kinase R (PKR), retinoic acid-inducible gene I (RIG-I), and the melanoma differentiation-associated gene 5 (MDA5)—mitochondrial antiviral signaling protein (MAVS)—stimulator of IFN genes (STING) pathways, and Toll-like receptor 3 (TLR3)37. We used the CRISPR/Cas9 approach to generate stable IEC4.1 cells deficient in Ddx58 (encoding Rig-I), Ifih1 (encoding Mda5), Mavs, or Eif2ak2 (encoding Pkr) (Fig. 5a). We took the siRNA approach to knockdown Tlr3 in IEC4.1 cells (Fig. 5b). Knockout of Ddx58, Mavs, or Eif2ak2 in IEC4.1 cells completely blocked C. parvum- and CSpV1-dsRNA-induced upregulation of Ifnb1 (Fig. 5c). Knockout of Ifih1 or siRNA knockdown of Tlr3 failed to block C. parvum- and CSpV1-dsRNA-induced Ifnb1 expression (Fig. 5c). Moreover, inhibition of Sting signaling by the inhibitor C-17838 or Pkr signaling by C-1639 partially blocked C. parvum- and CSpV1-dsRNA-induced Ifnb1 expression (Fig. 5d). Using the crosslinking RNA-binding protein immunoprecipitation (RIP) assay, we detected a physical association between CSpV1-dsRNAs with Pkr, Rig-I, or Mavs in cells transfected with CSpV1-dsRNAs (Fig. 5e) or cells following C. parvum infection for 24 h (Fig. 5f). Of note, although knockout of Ifih1 failed to block C. parvum- and CSpV1-dsRNA-induced Ifnb1 expression (Fig. 5c), we still detected a physical association between CSpV1-dsRNAs with Mda5 in infected cells or cells transfected with CSpV1-dsRNAs (Fig. 5e, f). These findings suggest that C. parvum infection triggers host type I IFN response through CSpV1-dsRNA-mediated activation of Pkr and Rig-I/Mavs/Sting signaling (Fig. 5g).
Suppression of cellular response to IFN-γ stimulation with the involvement of type I IFN signaling in infected host cells
Pretreatment of IEC4.1 cells with IFN-γ promoted host defense to C. parvum infection (Fig. 6a). Consistent with previous reports24,40, we detected an impaired cellular response to IFN-γ in cells after C. parvum infection. Compared with that in uninfected cells, a decrease in IFN-γ-induced expression of Ido1 was observed in IEC4.1 cells first exposed to C. parvum for 24 h followed by IFN-γ stimulation for additional 2 h (Fig. 6b). Such a decreased IFN-γ response was not observed in infected Ifnar1−/− IEC4.1 cells (Fig. 6b). Knockdown of CSpV1-dsRNA delivery in infected IEC4.1 cells, through host cell transfection of the specific siRNA combinations (siPOOLs), restored the induction of Ido1 gene expression in host cells following C. parvum infection (Fig. 6c). RNA-Seq analysis revealed no significant changes in the expression levels of key components associated with the IFN-γ signal pathway, including downstream JAK/STAT signaling between infected IEC4.1 and Ifnar1−/− IEC4.1 cells, as well as ileal epithelium from infected Ifnar1fl/fl and Villin.Ifnar1−/− neonates (Fig. 6d). A similar protein content of the IFN-γ receptor Ifngr1 subunit was detected by Western in IEC4.1 and IEC4.1-Ifnar1−/− cells (Fig. 6e); ileal epithelium of Ifnar1fl/fl and Villin.Ifnar1−/− neonates showed a similar protein content (Fig. 6e) and a similar staining pattern for Ifngr1 (Fig. 6f). Decreased expression levels of several other selected IFN-γ-stimulated genes, including Igtp, Irf8, and Ifi47, were further observed in IEC4.1 cells first exposed to C. parvum for 24 h followed by IFN-γ stimulation for additional 2 h (Supplementary Fig. 5a). Suppression of expression of Igtp and Irf8 (but not Ifi47) was observed in IEC4.1 cells first treated with IFN-α for 8 h followed by IFN-γ stimulation for additional 2 h in the absence of IFN-α (Supplementary Fig. 5b). Together, these data suggest that suppression of cellular response to IFN-γ stimulation with the involvement of type I IFN signaling in infected cells but such a suppressive effect appears not due to the direct effect of type I IFN signaling on the expression of key components of the IFN-γ signaling cascade in host cells.
Effects of anti-viral reagents and anti-parasite drugs on C. parvum infection of intestinal epithelium
Consistent with the results from previous studies41, a small but significant decrease in the infection burden was detected in cultured human intestinal epithelial HCT-8 cells treated with the antiviral reagents, Indinavir or Ribavirin (Fig. 7a). Cells were first exposed to C. parvum infection for 4 h (for parasite attachment and invasion)16,28 and then exposed to Indinavir or Ribavirin for additional 20 h followed by measurement of infection burden. Antiparasitic reagent paromomycin was used as the positive control41. A further decrease in infection burden was observed in cells treated with a combination of paromomycin with Indinavir or Ribavirin (Fig. 7a). Using frozen intestinal tissues from an existing human tissue bank (University of Nebraska Medical Center), we successfully developed 3D enteroids and 2D monolayers for C. parvum ex vivo infection (Fig. 7b, c). Induction of type I and III IFNs and Type I/III IFN-stimulated genes was detected in human 2D monolayers following C. parvum infection, further confirming the activation of type I and III IFN signaling in infected human intestinal epithelial cells (Fig. 7d). A significant decrease in the infection burden was detected in the human 2D monolayers treated with the antiviral reagents, Indinavir or Ribavirin (Fig. 7e). A further decrease in infection burden was observed in the monolayers treated with a combination of paromomycin with Indinavir or Ribavirin (Fig. 7e). Antiparasitic and antiviral drugs were administered to C57BL/6 neonates of 5 days old at 2 h after oral gavage of C. parvum oocysts, followed by a daily administration of the drugs for 3 days. Decreased infection burden was detected in the animals received paromomycin or Ribavirin, but not Indinavir (Fig. 7f, g). A further decrease in infection burden was observed in the intestinal epithelial tissues from animals received a combination of paromomycin and Ribavirin (Fig. 7f, g).
Discussion
Our data demonstrate that Cryptosporidium infection induces all type IFN responses in the intestinal epithelium in neonatal mice, including production of three types of IFNs and upregulation of many type I/III IFN- and IFN-γ-stimulated genes. We also detected increased expression of several type I IFNs in the intestinal epithelial cell cultures infected in vitro. More importantly, a majority of genes whose expression levels were altered in infected Ifnar1fl/fl mice showed no changes in the infected conditional intestinal epithelial cell Villin-Ifnar1−/− neonates, further supporting a strong type I IFN-mediated gene transcription in the infected intestinal epithelium. Type I and III IFNs bind to different receptors but activate a similar intracellular signaling cascade and induce highly similar transcriptional responses7,8. Suppression of type I/III-stimulated gene upregulation in intestinal epithelium isolated from the infected Villin-Ifnar1−/− mice also suggests that type I IFN-stimulated gene transcription in intestinal epithelial cells could not fully be complemented by type III IFNs. Thus, Cryptosporidium infection can induce type I IFN produce from intestinal epithelial cells and activation of type I IFN signaling in intestinal epithelial cells contributes significantly to the transcriptomic alterations in the infected intestinal epithelium, including transactivation of many type I/III-stimulated genes, at least during the early infection stage in neonatal mice.
Current understanding of the role for type I IFN signaling in intestinal anti-Cryptosporidium defense remains obscure. Previous studies demonstrated that pre-treatment of epithelial cells with IFN-α before exposure to C. parvum could modestly inhibit parasite invasion of host cells in vitro14. Type I IFN response reduced oocyst shedding and symptoms but not intestinal parasite burden following C. parvum infection in IFN-γ KO mice17. Gibson et al. recently reported that Cryptosporidium shedding was significantly reduced in the Ifnar1−/− mice compared to wild-type animal12. Whether this resistance to infection is due to loss of type I IFN signaling in intestinal epithelial cells or in other cell types such as T cells is unclear. Our findings support that intestinal epithelial conditional Ifnar1−/− mice are resistant to C. parvum infection, indicating a detrimental effect of type I IFN signaling in intestinal epithelial cells on intestinal anti-C. parvum defense. Therefore, although all three types of IFN signaling are activated in the neonatal intestinal epithelium following infection, each type of IFNs displays distinct roles in intestinal immunity to Cryptosporidium infection. Whereas type II and III IFNs show a profound protective role in control the infection, type I IFN signaling in the intestinal epithelial cells displays a pro-parasitic effect potentially through suppression of IFN-γ-mediated epithelial cell anti-Cryptosporidium defense.
Our observation of resistance to C. parvum intestinal infection in conditional intestinal epithelial Ifnar1−/− neonatal mice, but not in vitro infection in cultured cells lacking Ifnar1, suggests that type I IFN signaling may modulate intestinal anti-Cryptosporidium defense induced by determinants from other cell types during infection in vivo. One possible mechanism could be the potential impact of type I IFN response on type III IFN-mediated epithelial defense. Of note, no obvious induction of Ifnl2/3 genes and produce of IFN-λ2/3 proteins were observed in the IEC4.1 cells cultures following infection in vitro. While originally considered to be redundant to type I IFNs, type III IFNs have now been shown to play a unique role in protecting mucosal surfaces against pathogen challenges7. Our findings provide additional evidence that type I and III IFNs have distinct functions in intestinal epithelial cells, even though they stimulate a similar intracellular signaling cascade and an indistinguishable gene expression profile in the cells9. Indeed, type III IFNs show a profound protective role in control Cryptosporidium infection as mice lacking the type III receptor, Ifnlr1, showed an increase of parasite shedding following infection12. Future investigations on Cryptosporidium infection using conditional intestinal epithelial Ifnlr KO animals should clarify these possibilities.
Previous studies have demonstrated a dysregulation of IFN-γ-mediated signaling pathway in intestinal epithelial cells by Cryptosporidium parvum infection5,18,19. Our data support that activation of type I IFN signaling may be involved in C. parvum-induced dysregulation of IFN-γ response in intestinal epithelial cells. Whereas parasite attachment and host cell invasion were similar in cultured IEC4.1 and IEC4.1-Ifnar1−/− cells, we detected an impaired response to IFN-γ stimulation in infected IEC4.1 cells but not infected IEC4.1-Ifnar1−/− cells. Similarly, pre-treatment of cells with type I IFNs (IFN-α) also attenuated the cellular response to IFN-γ stimulation. Thus, we favor the hypothesis that activation of type I IFN signaling following C. parvum infection may cause dysregulation of IFN-γ-mediated cell response in intestinal epithelial cells, resulting in detrimental effect on intestinal anti-C. parvum defense. It is well-documented that the production of type I IFNs could serve as a double-edged sword: type I IFNs provide early resistance against acute viral infections but are detrimental to the host during certain bacterial infections and chronic viral infections8,42. Moreover, these “miscellaneous upregulated genes” whose expression levels were further enhanced in infected Villin.Ifnar1−/− mice may also contribute to the resistance to C. parvum infection in the Villin.Ifnar1−/− neonatal mice. Whether proteins coding by these misc. upregulated genes can directly regulate intestinal epithelial cell anti-C. parvum defense, how the type I IFN signaling may modulate their expression in the intestine following infection, and whether the microbiota composition may play a role are important questions to be addressed.
Another key finding of this study is host delivery of CSpV1-dsRNAs and its association with type I IFN signaling activation in epithelial cells following C. parvum infection. Due to lack of the machinery for mammalian cell entry, CSpV1 is transmitted only by intracellular routes20 and thus, it is unlikely that CSpV1 virus can directly infect intestinal epithelial cells. The predominant cytoplasmic localization of CSpV1-dsRNAs in infected cells, not in cells exposed to heat-treat parasites, indicates that direct parasite-host interaction is required for CSpV1-dsRNA delivery. CSpV1-dsRNAs in C. parvum are mainly localized to the sporozoite apical organelle region21. At the initial stage of host cell internalization, C. parvum sporozoites attach to the epithelial cell membrane of at their apical tip28,30. Subsequent discharge of apical organelle rhoptry and microneme contents into the host cell, including several parasite proteins such as ROP1 (cgd3_1770) and ROP3 (cgd3_1710), may contribute to parasite entry and parasitophorous vacuole formation30,43,44. Therefore, it is plausible that CSpV1-dsRNAs or CSpV1 virus particles are delivered into host cells in concert with parasite apical organelle discharge. Consequently, delivery of CSpV1-dsRNAs activates type I IFN signaling in infected cells through activating the Pkr and Rig-I/Mavs/Sting signal pathways. A comparable induction of type I IFN gene transcription was detected in cells transfected with CSpV1-dsRdRp or CSpV1-dsCA fragment, suggesting that CSpV1-dsRNAs trigger host type I IFN gene transcription in a sequence-independent manner. Of note, C. parvum infection triggers host type I IFN response through CSpV1-dsRNA-mediated activation of Pkr and Rig-I/Mavs/Sting signaling, in a TLR3-independent manner. TLR3 recognition accounts for intestinal type III produce12 but is not required for type I IFN response in infected intestinal epithelial cells, suggesting distinct mechanisms to trigger type I and III signaling in host cells following Cryptosporidium infection.
CSpV1 is the only virus identified so far in various Cryptosporidium spp20. and whether CSpV1 has an impact on parasite pathogenesis is unclear. The dsRNA viruses of some other parasites can affect key aspects of parasite biology, including their virulence45,46,47. The presence of Leishmania RNA virus 1 (LRV1) and Trichomonas vaginalis virus is strongly linked to parasite pathogenesis in human infections by Leishmania guyanensis and Trichomonas vaginalis48,49. LRV1 within the Leishmania parasite exacerbates the pathogenesis of human leishmaniasis48,49, and the presence of the virus correlates with drug treatment failures48. Importantly, the fecundity of C. parvum appears to be correlated with its intracellular CSpV1 levels50. We speculate that high levels of type I IFN signaling in the intestinal epithelium induced by CSpV1-dsRNAs following C. parvum infection triggers the host’s antiviral response preferentially over a strong antiparasitic response, which in turn benefits parasite survival. Accordingly, CSpV1 could be a new therapeutic target for cryptosporidiosis. A modest effect of antiretroviral protease inhibitors, including indinavir which targets a HIV protease in HIV virus51, on the development of cryptosporidiosis was previously reported41,52,53. We observed that ribavirin, antiviral reagent that interferes with the synthesis of viral mRNA54, can also promote the inhibitory effect of antiparasitic drugs on C. parvum infection of intestinal epithelium. Although the underlying mechanisms are still unclear, our finding on the role of CSpV1 virus in attenuating host anti-parasitic defense may provide a mechanistical clue for the inhibitory effects of antiviral reagents on Cryptosporidium infection. Interestingly, the only FDA-approved anti-C. parvum drug nitazoxanide also displays some effects in the treatment of chronic hepatitis B and C 55,56.
To conclude, our findings in this study indicate a detrimental effect of type I IFN signaling in intestinal epithelial cells on intestinal anti-C. parvum defense and reveal an important strategy of immune evasion by the parasite through the delivery of CSpV1-dsRNAs. Better understanding the functional impact of CSpV1-mediated type I IFN responses on mucosal anti-C. parvum immunity would provide mechanistic insights into host-C. parvum interactions, relevant to future development of novel therapeutic strategies.
Methods
Ethics statement
All research studies involving the use of animals were reviewed and approved by the Institutional Animal Care and Use Committees of the Creighton University School of Medicine at Omaha, Nebraska and were carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals. The 3D enteroids are derived from human intestinal tissues from an existing human tissue bank (IRB protocol #714-18; University of Nebraska Medical Center, NE, USA).
C. parvum, cell lines, and human intestinal epithelial monolayers
C. parvum oocysts were harvested from calves inoculated with the Iowa strain originally obtained from Dr. Harley Moon at the National Animal Disease Center (Ames, IA, USA) and purchased from a commercial source (Bunch Grass Farms, Deary, ID, USA). For in vitro, ex vivo, and in vivo infection, oocysts were treated with 1% sodium hypochlorite (Spectrum Chemical, catalogue no, S1669) on ice for 20 min and washed twice with 1x phosphate-buffered saline (PBS, IBI Scientific, catalogue no. IB70165) followed by washing twice with DMEM/F12 Medium (ATCC, catalogue no. 30-2006).
The neonatal murine intestinal epithelial cell line (IEC4.1) was a kind gift from Dr. Pingchang Yang (McMaster University, Hamilton, Canada). The human intestinal adenocarcinoma HCT-8 cells were obtained from ATCC (CCL-244) and cultured in RPMI-1640 Medium (ATCC, catalogue no. 30-2001) supplied with 10% FBS (Gibco, catalogue no. 10082147) and 100 U/ml penicillin-streptomycin (Gibco, catalogue no. 15140-122). The human 2D intestinal epithelial monolayers were generated from 3D enteroids derived from human intestinal tissues from an existing human tissue bank (IRB protocol #714-18; University of Nebraska Medical Center, NE, USA). Intestinal villus/crypt components were isolated and cultured using an approach modified from our previous studies in mice57. Briefly, human biopsies were washed once with ice-cold Dulbecco’s phosphate buffered saline without Ca2+ and Mg2+ (DPBS, Gibco, catalogue no. 14190144) and twice with ice-cold chelation buffer [1x DPBS, 2% sorbitol (Fisher Scientific, catalogue no. BP439-500), 1% sucrose (Fisher Scientific, catalogue no. BP220-1), 1% bovine serum albumin (BSA, Thermo Scientific, catalogue no. 37525)]. Washed biopsies were immersed in freshly prepared ice-cold chelation buffer with 2 mM EDTA (Sigma-Aldrich, catalogue no. E9884), shaken gently for 30 min at 4 °C on a Pulsing Vortex Mixer (Fisher Scientific, catalogue no. 02-215-422) and then vigorously until it became mostly opaque with dislodged crypt and villus particles. The crypt pellets were collected after quick centrifugation for 30 s and washed in ice-cold chelation buffer for three times. The pellets were then resuspended in ice-cold basement membrane matrix comprising complete human minigut 3D medium and Matrigel Membrane Matrix (Fisher Scientific, catalogue no. CB-40234) in 1: 1 ratio v/v, and plated in 37 °C pre-warmed 35 mm dishes (30 μL mix per spot, 12 spots) or 60 mm dishes (30 spots). The basement membrane matrix was overlaid with complete human minigut 3D medium after incubation in 37 °C, 5% CO2 for 20 min. Complete human minigut 3D medium was prepared in a base solution of Wnt-3A conditioned medium and human minigut basic medium in 1: 1 ratio v/v with 1 μg/ml hR-spondin1 (Peprotech, catalogue no. 120-38), 100 ng/ml hNoggin (Biotech, catalogue no. 6057-NG), 50 ng/ml hEGF (Sigma-Aldrich, catalogue no. E9644), 500 nM A-83-01 (Tocris Bioscience, catalogue no. 2939), 10 nM [Leu]15-Gastrin1 (Sigma-Aldrich, catalogue no. G9145), 10 nM Nicotinamide (Sigma-Aldrich, catalogue no. N0636), and 1 mM N-Acetylcysteine (Sigma-Aldrich, catalogue no. A9165). Wnt-3A conditioned medium was generated in house with L-Wnt3a cell line (ATCC, catalog no. CRL-2647) following the manufacturer’s instructions. Human minigut basic medium comprises 1x Advanced DMEM/F12 Medium (Gibco, catalogue no. 12634-010), 1x glutamine (Gibco, catalogue no. 35050-061), 100 U/ml penicillin-streptomycin, 1x N2 supplement (Gibco, catalogue no. 17502-048), 1x B27 supplement (Gibco, catalogue no. 17504-044), and 1% BSA. All above steps were carried out in buffers kept on ice. Complete human minigut 3D culture medium was changed every other day. For enteroid propagation, complete human minigut 3D medium should be freshly prepared. Monolayer cultures were derived from 3D enteroids as previously described16,58.
Generation of conditional intestinal epithelial Ifnar1 −/− mice
The conditional intestinal epithelial type I IFN receptor Ifnar1 KO mice (Villin.Ifnar1−/− mice) were generated from the cross-breeding of the Ifnar1fl/fl mice (JAX, catalogue no. 028256, which carry a floxed allele with loxP sites flanking exon 3 of the type-I-IFN-α/β receptor gene) and the Vil1-cre mice (JAX, catalogue no. 004586, which contain the villin1 promotor directing expression of mouse Cre recombinase in villus and crypt epithelial cells of the intestine). Mice were housed and bred in the animal care facility under a 12/12 h light/dark cycle, 20 °C ambient temperature with humidity around 50%. Both breeding pairs of both strains were purchased from the Jackson Laboratory (Bar Harbor, ME) and cross-bred according to the manufacturer’s guide. The genotypes of littermates were identified using mouse tail DNA for PCR using primers designed from the Jackson Laboratory: wild type (loxP 395 bp and Cre+, M1; loxP 325 bp and Cre−, M2), heterozygous (loxP 395/325 bp and Cre+, M3) and homozygous (loxP 325 bp and Cre+, M4). Lack of Ifnar1 exon3 expression in the intestinal epithelium of Villin.Ifnar1−/− mice was further confirmed by RT-qPCR (Supplementary Fig. 1a).
Reagents, infection models, antiparasitic and antiviral agents, and infection assays
C-178 was purchased from ProbeChem (catalogue no. PC-35310) and C-16 from Sigma-Aldrich (catalogue no. 527451). Models of intestinal cryptosporidiosis using intestinal epithelial cell lines and 2D epithelial monolayers were employed as previously described16,57,58. Infection was done in culture medium (DMEM/F12 with 100 U/ml penicillin-streptomycin) containing viable C. parvum oocysts (oocysts with host cells in a 1:4 ratio). A well-developed infection model of cryptosporidiosis in neonatal mice was used for in vivo experiments59,60. Briefly, neonates (5 days old, both male and female animals were randomly used) were received C. parvum oocysts by oral gavage (106 oocysts per animal). Mice receiving 1× PBS by oral gavage were used as control. For animals treated with antiparasitic and antiviral reagents, first treatment was administered 2 h after C. parvum infection, followed by daily for 3 days. Animals received antiparasitic agent (paromomycin, Sigma-Aldrich, catalogue no. P9297, 25 mg/kg body weight/day, P.O.) or antiviral agents (ribavirin, Sigma-Aldrich, catalogue no. R9644, 40 mg/kg body weight/day, P.O., or indinavir, Sigma-Aldrich, catalogue no. SML0189, 24 mg/kg body weight/day, I. P.) or a combination of antiparasitic and antiviral agents (paromomycin, 25 mg/kg body weight/day, with ribavirin, 40 mg/kg body weight/day; or paromomycin, 25 mg/kg body weight/day, with indinavir, 25 mg/kg body weight/day). At various time points after Cryptosporidium or vehicle/drug administration, ileum intestinal tissues (4 cm of small intestine tissue from the ileocecal junction) were collected from the animals (minimum of 6 mice from each group). Biochemical analysis, RT-qPCR, immunofluorescent staining, and histology were performed as previously reported57,58. For in vitro and ex vivo infection, cell cultures or 2D monolayers were exposed to C. parvum infection for 4 h and culture medium was changed to remove free parasite. Paromomycin (500 µg/ml), indinavir (200 µg/ml), ribavirin (20 µg/ml) or a combination of antiparasitic and antiviral agents was then added to the medium and cultured for additional 20 h (a total 24 h p.i.) followed by infection burden measurement. Infection burden was quantified by measuring levels of C. parvum 18 s (cp18s) or Hsp70 (cpHsp70) gene13. Immunofluorescent staining of C. parvum was carried out using the rabbit antiserum against C. parvum membrane proteins as previously described61,62. For parasite counting, the number of intracellular parasites in each intestinal section following immunofluorescent staining of C. parvum was determined from 20 randomly chosen fields at 60X total magnification and presented as average parasite number per 60X field. Other antibodies used for immunofluorescent staining include anti-PCNA antibody (Cell Signaling, catalogue no. 13110, 1:1000), anti-EpCAM antibody (Abcam, catalogue no. ab71916, 1:200), anti-Lysozyme antibody (Abcam, catalogue no. ab108508, 1:500), anti-Villin antibody (Santa Cruz, catalogue no. sc-58897, 1:200), anti-mouse-Cruz fluor 488-conjugated (Santa Cruz, catalogue no. sc-516176, 1:200), anti-rabbit-Cruz fluor 488-conjugated (Santa Cruz, catalogue no. sc-516248, 1:200), anti-bovine Alexa fluor 594-conjugated (Jackson Immuno Research lab, catalogue no. 101-585-003, 1:250).
RT-qPCR
For quantitative analysis of RNA expression, comparative real-time PCR was performed as previously reported57,58, using the iTaq™ Universal SYBR® Green Supermix (Bio-rad, Hercules, CA, USA, catalogue no. 1725124). Briefly, total RNA was isolated using TRIzol™ Reagent (ThermoFisher Scientific, catalogue no. 15596018) and possible remaining DNA was removed with the DNA-free™ DNA Removal Kit (ThermoFisher Scientific, catalogue no. AM1906). The amount of RNA was measured by quantitative, strand-specific RT-PCR using the iCycler iQ Real-time detection system (Bio-Rad CFX Manager v3.1) with 25 ng of template cDNA from reverse transcription for each RNA gene of interest. The expression level of each RNA was calculated using the ΔΔCt method and normalized to Gapdh. All sequences of PCR primers are listed in Supplementary Data 4.
siRNAs, CRISPR/Cas9 and stable KO IEC4.1 cells
Custom-designed RNA oligos against CSpV1-dsRNAs and scrambled siRNA-control were synthesized by Integrated DNA Technologies (Coralville, Iowa) and siRNA to Tlr3 was from Santa Cruz (sc-40259). Sequences of siRNAs are listed in Supplementary Data 4. siRNAs were transfected into cells with Lipofectamine™ RNAiMAX Transfection Reagent according to the manufacturer’s protocol (ThermoFisher Scientific, catalogue no. 13778150). Stable IEC4.1 cell lines with deficiency in Ifnar1, Ddx58, Eif2ak2, Mavs or Ifih1 genes were generated through transfection of cells with the CRISPR/Cas9 KO and the HDR plasmid, respectively, according to the manufacturer’s instruction and as previously described16. Briefly, the gene-specific CRISPR/Cas9 KO plasmids and its corresponding HDR plasmids were co-transfected into the cells using the UltraCruz® Transfection Reagent (Santa Cruz, catalogue no. sc-395739) and plasmid transfection medium (Santa Cruz, catalogue no. sc-108062), followed by selection with puromycin (5 µg/ml, Santa Cruz, catalogue no. sc-108071). The IFN-α/βRα CRISPR Plasmids(m) (sc-421047), Rig-I CRISPR Plasmids(m) (sc-432915), PKR CRISPR Plasmids(m) (sc-422410), Mavs CRISPR Plasmids(m) (sc-432790), Mda5 CRISPR Plasmids(m) (sc-427977) and their corresponding HDR plasmids were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). The primers used to screen the CRISPR/Cas9 KO cell lines are listed in Supplementary Data 4.
Cytoplasmic extracts, Western blot, and dot blot
Cells were grown to 80% confluence, treated, and collected at designed time points. Cells were washed with 1× PBS and detached with 0.05 M EDTA in 1× PBS. Detached cells were pelleted by centrifugation at 100 × g for 3 min. Cytoplasmic extracts were prepared using the standard approach32. Briefly, pelleted cells were resuspended in lysis buffer [10 mM Tris-HCl (Fisher Scientific, catalogue no. BP-152-1), pH 7.4, 10 mM NaCl (Fisher Scientific, catalogue no. S271-3), 3 mM MgCl2 (MP Biomedicals, Irvine, CA, catalogue no. 191421), 100 U/ml RNase inhibitor (Thermo Fisher Scientific, catalogue no. AM2696)] and incubated on ice for 10 min. Cell lysates were centrifuged at 100 g for 2 min at 4 °C and the supernatants saved as cytoplasmic extract.
Western blot and dot blot were performed as reported in our previous study57,58. Briefly, cell lysates were collected and SDS-PAGE gel electrophoresis was used to separate denatured proteins on 10% TGS gels. Proteins were transferred to Immun-Blot® PVDF Membrane (Bio-Rad, catalogue no. 1620177). The membranes were blocked with Blotting Grade Blocker Non Fat Dry Milk (Bio-Rad, catalogue no. 1706404XTU) for 1 h and then incubated with the primary antibody. After washing with 1× Tris Buffered Saline with 0.1% Tween® 20 for three times (10 min each), secondary antibody was applied. For dot blot, RNA was spotted on the Amersham Hybond-N+ membrane (Avantor, catalogue no. 95038-336) followed by UV crosslinking and antibody incubation. Membranes were washed and developed using Clarity Western ECL Substrate (Bio-Rad, catalogue no. 1705061) following the manufacturer’s instructions. Blot imaging was obtained and analyzed using the AlphaEase FC Software v6.0.2. The following antibodies were used for blotting: anti-Isg15 (Santa Cruz, catalogue no. sc-166755, 1:100), anti-Usp18 (Biomatik, catalogue no. CAC08089, 1:500), anti-Ifngr1 (Thermo Fisher Scientific, catalogue no. MA5-35147, 1:500), anti-Mda5 (Abcam, catalogue no. Ab79055, 1:50), anti-Rig-I (Proteintech, catalogue no. 20566-1-AP, 1:500), anti-Mavs (Proteintech, catalogue no. 14341-1-AP, 1:2000), anti-Pkr (Santa Cruz, catalogue no. sc-6282 AC, 1:200), anti-viral dsRNAs (J2) (Millipore, catalogue no. MABE1134, 1:60), anti-Gapdh (Santa Cruz, catalogue no. sc-32233, 1:1000), anti-rabbit-HRP-conjugated secondary antibody (Santa Cruz, catalogue no. sc-2357, 1:5000), anti-mouse-HRP-conjugated secondary antibody (Santa Cruz, catalogue no. sc-516102, 1:5000), and anti-viral dsRNA (J2) (Millipore, catalogue no. MABE1134, 1:60).
RNA-Seq and bioinformatics analysis
Total RNAs were extracted using Rneasy Mini Kit (QIAGEN, catalogue no. 74104). Transcriptome sequencing and result processing were performed by the BGI Americas Corporation (Cambridge, MA) with DNBSEQ™ sequencing technology platforms as previously reported40. The quality of RNA was evaluated with a 2100 Bioanalyzer (Agilent Technologies, USA) and Samples classified as good RNA quality had a similar RNA pattern at visual inspection in the Bioanalyzer, an RNA Integrity Number (RIN) > 7 and a concentration >20 ng/ul. Total RNA was fragmented into short fragments and mRNA was enriched using oligo (dT) magnetic beads, followed by cDNA synthesis. Double-stranded cDNA was purified and enriched by PCR amplification, after which the library products were sequenced using DNBSEQ-500 platform with read length of PE100, averagely generated about 4.94 Gb bases per sample. Low quality reads (more than 20% of the bases qualities are lower than 10) and reads with adaptors and reads with unknown bases (N bases more than 5%) were filtered to get the clean reads using the internal software SOAPnuke (version: v1.5.2, parameters: -I 15-q0.5-n0.1) in the DNBSEQ-500 platform. After filtering, the clean reads were stored in FASTQ format and read alignments were performed by HISAT2 (version: v2.0.4; parameters: -phred64-sensitive-no-discordant-no-mixed-I 1-X 1000) and Bowtie2 (version: v2.2.5) referring to the Mus_musculus genome (GCF_000001635.26_GRCm38.p6) from NCBI database. Raw RNA-seq reads were then filtered out the unreliable sites to remove low-quality reads using the GATK program for each sample. Final gene reads were normalized to RPKM (reads per kilobase of transcript, per Million mapped reads) as relative gene expression levels.
Differential expression analysis was performed by the BGI, using the Dr. TOM data system. Altered (upregulated or downregulated) expression of genes was expressed as log2FC or log10FC, which represents log-transformed fold change (log2FC = log2[B] − log2[A] or log10FC = log10[B] − log10[A], while A and B represent values of gene expression from different treatment conditions). Statistical significance was determined based on two-tailed Wald test followed by Benjamini–Hochberg procedure for multiple comparison correction63. The Venny 2.1 software was used to identify genes common to the different treated groups64. Gene set enrichment analysis (GSEA) was run using pre-ranked mode for all genes by R package “clusterProfiler” based on log2FC derived from the differential expression analysis by weighted Kolmogorov–Smirnov test and p value was adjusted by Benjamini–Hochberg method65. Visualization of GSEA results were achieved by R package “ggplot2”66. Volcano plot was generated using R package “ggplot2” with log2FC and adjusted p value derived from differential expression analysis. Heatmaps were generated using R package “ComplexHeatmap”67. Expression z-score was calculated based on the relative gene expression levels68.
Synthesis of CSpV1-dsRNAs and transfection
C. parvum sporozoite total RNA was isolated using TRIzol (Invitrogen, catalogue no. 15596-026). CSpV1-dsRNAs were reverse-transcribed to cDNAs using MMLV-RT (Thermo Fisher Scientific, catalogue no. 28025-013) with sporozoite total RNA as template and specific primers for CSpV1dsRNA1_RdRpFull_R1 (5′-GGCCGATCATAGCTACTCC-3′), CSpV1dsRNA2_CAFull_R1 (5′-GGCCGATCATAGCTACTTTCATAAG-3′). Amplification of full length CSpV1-RdRp and CSpV1-CA was performed using the Platinum™ Taq DNA Polymerase High Fidelity system (Thermo Fisher Scientific, catalogue no. 11304011) with reverse-transcribed cDNA templates and specific primers CSpV1dsRNA1_RdRpFull_F1 (5′-GGAAAGAAGCATAGCTCAATTCTC-3′), CSpV1dsRNA1_RdRpFull_R1 (5′-GGCCGATCATAGCTACTCC-3′), CSpV1dsRNA2_CAFull_F1 (5′-GGAAAAGAGGAACAGCAACTC-3′), CSpV1dsRNA2_CAFull_R1 (5′-GGCCGATCATAGCTACTTTCATAAG-3′). Amplified products were sent to Genewiz (South Plainfield, NJ) for Sanger Sequencing. Plasmid constructs for CSpV1-dsRdRp in vitro transcription was generated by cloning PCR-amplified full length CSpV1-RdRp into the pSPT18 vector (Sigma-Aldrich, catalogue no. 10999644001), into the HindIII and KpnI sites, in the orientation for SP6 transcription. PCR-amplified full length CSpV1-CA was A-tailed with Taq DNA Polymerase (NEB, catalogue no. M0267) and cloned into the pTargeT™ vector using the pTargeT™ Mammalian Expression Vector System (Promega, catalogue no. A1410). Single-strand CSpV1-RNAs were synthesized using SP6/T7 Transcription Kit (Sigma-Aldrich, catalogue no. 10999644001) with the pSPT18-CSpV1-RdRp and pTargeT™-CSpV1-CA plasmid constructs as templates. Sense and anti-sense CSpV1-ssRNAs were annealed to CSpV1-dsRdRp and CSpV1-dsCA respectively by 95 ˚C incubation for 5 min and gradual cooling down over 2 h. CSpV1-ssRNAs and CSpV1-dsRNAs were transfected to IEC4.1 cells with Lipofectamine 2000 (Thermo Fisher Scientific, catalogue no. 11668019) for 24 h. A non-specific scrambled dsRNA was transfected at the same time in control group. For specific details of the specific primers and RNA sequences, see the Supplementary Data 4.
Fluorescent in situ hybridization
DIG-labeled antisense CSpV1-dsRNAs were synthesized employing SP6/T7 Transcription Kit (Sigma-Aldrich, catalogue no. 10999644001) as described above. Cells were incubated and treated on Nunc™ Lab-Tek™ II Chamber Slide™ System (Thermo Scientific, catalogue no. 154534). The cells were fixed in 4% paraformaldehyde (Thermo Fisher Scientific, catalogue no. 28908) with 5% acetic acid (Fisher Scientific, catalogue no. BP2401-212) and 0.9% NaCl for 30 min, and washed twice with diethylpyrocarbonate (DEPC, Sigma-Aldrich, catalogue no. 06756) treated 1× PBS. Fixed cells were permeabilized in 0.1% Triton X-100/PBS (Bio-rad, catalogue no. 1610407) with 100 U/ml RNase inhibitor for 10 min and washed three times with 1× DEPC-PBS. Hybridization occurred in DIG Easy Hyb Granules (Sigma-Aldrich, catalogue no. 11 796 895 001) according to manufacturer’s guide. Blocking of hybridization was performed using Blocking Reagent (Sigma-Aldrich, catalogue no. 11 096 176 001). Subsequent immunofluorescence staining was performed with Anti-Digoxigenin-Fluorescein (Sigma-Aldrich, catalogue no. 11 207 741 910) in a concentration of 1:200 in 0.5% BSA/PBS for 1 h. The slides were washed twice with 1x DEPC-PBS and then mounted using Antifade Mounting Medium with DAPI (Vector, catalogue no. H-1200).
RIP assay
The formaldehyde crosslinking RIP was performed as described16. Briefly, cells were treated with 0.3% Formaldehyde (Fisher Scientific, catalogue no. BP531-500) at 37 °C for 10 min. Crosslinking reactions were quenched by incubation with 0.25 M glycine (IBI Scientific, catalogue no. IB70194) at room temperature for 5 min. Cells were harvested by centrifugation at 800 × g for 4 min at 4 °C and washed twice with ice-cold 1× PBS. Cell pellets were resuspended in whole cell extract buffer [20 mM HEPES pH 7.4 (Teknova, catalogue no. H1030), 0.2 M NaCl, 0.5% Triton X-100, 10% glycerol (Sigma-Aldrich, catalogue no. G7757), 1 mM EDTA, 1 mM EGTA (Sigma-Aldrich, catalogue no. E3889), 1 mM DTT (Fisher Scientific, catalogue no. BP1725), cocktail protease inhibitor (Sigma-Aldrich, catalogue no. 11697498001) and 100 U/ml RNase inhibitor]. The crosslinked complexes in cell lysates were solubilized by three rounds of sonication, 20 s each, in a Microson XL2007 ultrasonic homogenizer with a microprobe at an amplitude setting of 7 (output, 8–9 W). Insoluble materials were removed by microcentrifugation at 16,100 × g for 20 min at 4 °C. Lysates were precleaned with 1× PBS washed Magna CHIP Protein A + G Magnetic Beads (Sigma-Aldrich, catalogue no. 16-663) and isotype IgG. The precleaned lysate was then diluted with whole cell extract buffer in 1:1 ratio v/v, mixed with the specific antibody- or isotype IgG-coated beads, and incubated with rotation at 4 °C overnight, followed by two washes with whole cell extract buffer. The collected immunoprecipitated RNP complexes and input were digested in RNA PK Buffer pH 7.0 [100 mM NaCl, 10 mM Tris-HCl pH 7.0,1 mM EDTA, 0.5% SDS (Fisher Scientific, catalogue no. BP166500) and 100 U/ml RNase inhibitor] with 100 µg/ml Proteinase K (Thermo Scientific, catalogue no. E0491)] and incubated at 50 °C for 45 min with end-to-end shaking. Formaldehyde cross-links were reversed by incubation at 65 °C with end-to-end shaking for 4 h. RNA was extracted using the TRIzol™ Reagent and treated with DNA-free™ DNA Removal Kit. The presence of RNA was measured by RT-qPCR. Gene-specific PCR primer pairs are listed in Supplementary Data 4. The following antibodies were used: anti-Mda5 (ThermoFisher Scientific, catalogue no. 21775-1-AP,4 µg/ml), anti-Rig-I (Proteintech, catalogue no. 20566-1-AP, 4 µg/ml), anti-Mavs (Proteintech, catalogue no. 14341-1-AP, 4 µg/ml), anti-Pkr (Proteintech, catalogue no. 18244-1-AP, 4 µg/ml), anti-viral dsRNAs (J2) (Millipore, catalogue no. MABE1134, 4 µg/ml), anti-Mouse-IgG (Cell Signaling, catalogue no. 5415 S), anti-Rabbit-IgG (Cell Signaling, catalogue no. 3900S, 4 µg/ml).
ELISA
IEC4.1 cells were grown on 24 well plates to 80% confluency and were infected with C. parvum oocysts. At the indicated timepoint post infection, supernatants were collected and selected IFN protein levels were measured by mouse IFN-beta DuoSet ELISA (DY8234, R&D Systems), mouse IL28B/IFN-lambda 2/3 DuoSet ELISA (DY1789B, R&D Systems), and mouse IFN-gamma DuoSet ELISA (DY485, R&D Systems) according to the manufacturer’s instructions. Briefly, 96-well microplate was coated with Capture Antibody and incubated overnight at room temperature after sealing. Samples or standards were added to the processed microplate and incubated at room temperature for 2 h after sealing. After washing, the detection antibody was added to the microplate and incubated at room temperature for 2 h. Streptavidin-HRP was added and incubated at room temperature for 20 min. After washing, the plate was incubated with the Substrate Solution at room temperature for 20 min. Stop solution was then added and a microplate reader was used to determine the optical density of each well immediately.
Statistical and reproducibility
Statistical analysis was performed using GraphPad Prism 5 (GraphPad Software). All values are given as mean ± S.D. Means of groups were from at least three independent experiments or biological replicates. When two treatments were being compared the two-tailed unpaired Student’s t test was used; when more than two groups were being compared one-way or two-way ANOVA followed by Tukey’s HSD test were used. p values <0.05 were considered statistically significant. All the presented data and images have been independently repeated at least three times.
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Data availability
The RNA-seq data used in this study are deposited in the Gene Expression Omnibus (GEO) database repository under the accession numbers GSE147720, GSE164279, and GSE164316. Source data are provided with this paper.
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Acknowledgements
We thank Dr. Pingchang Yang (McMaster University, Hamilton, Canada) for sharing the IEC4.1 cells, Dr. Guan Zhu (Texas A&M University, College Station, TX) for providing the rabbit antiserum against C. parvum membrane proteins, and Dr. Dong-Er Zhang (Department of Pathology, University of California San Diego, La Jolla, California) and Dr. Jenny P. Ting (Department of Genetics, Center for Translational Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina) for their helpful comments on the study. This work was supported by funding from the National Institutes of Health (AI116323, AI136877, AI141325, and AI156370) to X-M.C. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center for Research Resources or the National Institutes of Health.
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X.-M.C. conceived, designed, and supervised the study with the help from M.B. on the CSpV1 virology and viral RNA transfection. S.D. performed most of the experiments and data analysis. W.H., A.-Y.G., M.L., Y.W., Z.X., and X.-T.Z. performed some experiments, helped with the experimental design and performance, analyzed the data, and provided analytical ideas. A.S.H. developed and provided the intestinal tissues from the human tissue bank and helped with data analyses. A.N. helped with the bioinformatics analysis. M.J., P.C.S., K.M.D., and J.K.S. provided critical comments and contributed to study design. S.D. and X.-M.C. wrote the manuscript with input from all authors.
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Deng, S., He, W., Gong, AY. et al. Cryptosporidium uses CSpV1 to activate host type I interferon and attenuate antiparasitic defenses. Nat Commun 14, 1456 (2023). https://doi.org/10.1038/s41467-023-37129-0
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DOI: https://doi.org/10.1038/s41467-023-37129-0
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