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| Open AccessTruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol
Single cell genomic analysis needs DNA amplification with high fidelity and accuracy. Here, the authors devise a novel multiple displacement amplification method called TruePrime that is based in Thermus thermophilusPrimPol and Phi29 DNA polymerase, and demonstrate its utility and accuracy.
- Ángel J. Picher
- , Bettina Budeus
- & Armin Schneider
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Calibrating genomic and allelic coverage bias in single-cell sequencing
Artifacts caused by whole-genome amplification bias are a recurrent challenge in single-cell sequencing analysis. Here, the authors develop statistical models and demonstrate an efficient strategy for controlling amplification errors by a joint analysis of single cell genomes.
- Cheng-Zhong Zhang
- , Viktor A. Adalsteinsson
- & J. Christopher Love
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Article
| Open AccessWhole-genome sequence-based analysis of thyroid function
Levels of circulating thyrotropin and free thyroxine reflect thyroid function, however, their genetic underpinnings remain poorly understood. Taylor et al. take advantage of whole-genome sequence data from cohorts within the UK10K project to identify novel variants associated with these traits.
- Peter N. Taylor
- , Eleonora Porcu
- & Pingbo Zhang