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Article
| Open Access
Wound infiltrating adipocytes are not myofibroblasts
Adipocyte plasticity during injury repair is debated. Here, the authors find that injury causes temporary adipocyte migration, but without lineage conversion to myofibroblasts. Distinct migration patterns, transcriptomics and ECM depositions indicate that adipocytes, unlike fibroblasts, do not contribute to fibrotic scars.
- Shruthi Kalgudde Gopal
- , Ruoxuan Dai
- & Yuval Rinkevich
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Article
| Open Access
Computational conjugate adaptive optics microscopy for longitudinal through-skull imaging of cortical myelin
The authors realize longitudinal deep-brain imaging through an intact mouse skull by constructing a high-speed reflection matrix microscope at 1.3 µm wavelength and developing a computational conjugate adaptive optics algorithm eliminating skull aberrations.
- Yongwoo Kwon
- , Jin Hee Hong
- & Wonshik Choi
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| Open Access
Traject3d allows label-free identification of distinct co-occurring phenotypes within 3D culture by live imaging
There are currently a lack of tools to detect heterogeneity in 3D cultures. Here the authors report Traject3d as a framework to identify heterogeneous states in 3D culture and to understand how these give rise to distinct phenotypes using label-free multi-day time-lapse imaging.
- Eva C. Freckmann
- , Emma Sandilands
- & David M. Bryant
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| Open Access
Nuclear speed and cycle length co-vary with local density during syncytial blastoderm formation in a cricket
Early in insect embryo development, many nuclei share one large cell, travel varied paths and self-organize into a single layer. Donoughe et al. illuminate this process with live-imaging, modeling, and experimental changes to the embryo’s shape.
- Seth Donoughe
- , Jordan Hoffmann
- & Cassandra G. Extavour
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Article
| Open Access
Non-canonical Wnt signaling promotes directed migration of intestinal stem cells to sites of injury
Stem cell migration is critical during adult tissue regeneration. Here, the authors demonstrate that enteroendocrine cells coordinate stem cell migration towards sites of injury in the Drosophila intestine by activating non-canonical Wnt signaling.
- Daniel Jun-Kit Hu
- , Jina Yun
- & Heinrich Jasper
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Article
| Open Access
Circa-SCOPE: high-throughput live single-cell imaging method for analysis of circadian clock resetting
Phase Transition Curves (PTCs) describe phase shifts of circadian oscillations due to a stimulus and they are important for studying circadian clock resetting. Here, the authors present a method for high-throughput reconstruction of PTCs using fluorescent live imaging and single-cell analysis.
- Gal Manella
- , Dan Aizik
- & Gad Asher
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Article
| Open Access
Multifocal imaging for precise, label-free tracking of fast biological processes in 3D
Multifocal imaging suffers from a number of limitations. Here the authors report an open-source 3D reconstruction algorithm to enable label-free tracking of spherical and filamentous structures which they use to characterise fluid flow and flagellar beating of human and sea urchin sperm.
- Jan N. Hansen
- , An Gong
- & Luis Alvarez
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Article
| Open Access
4D analysis of malaria parasite invasion offers insights into erythrocyte membrane remodeling and parasitophorous vacuole formation
Here, Geoghegan, Evelyn et al. provide a lattice light-sheet microscopy based 4D imaging pipeline to quantitatively investigate Plasmodium spp. invasion and show that the nascent parasitophorous vacuole is predominantly formed from host’s erythrocyte membrane and undergoes continuous remodeling throughout invasion.
- Niall D. Geoghegan
- , Cindy Evelyn
- & Kelly L. Rogers
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Article
| Open Access
Melanoma subpopulations that rapidly escape MAPK pathway inhibition incur DNA damage and rely on stress signalling
BRAF inhibitors are used to treat late-stage melanoma patients harbouring BRAF mutations. Here the authors track the responses of single melanoma cells to BRAF inhibitors and show that a subset of cells rapidly escapes drug via non-genetic mechanisms and incurs DNA damage.
- Chen Yang
- , Chengzhe Tian
- & Sabrina L. Spencer
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Article
| Open Access
Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin
Extensive scars develop in deep wounds as opposed to superficial wounds but it is unclear why. Here, the authors use live imaging of physiologic wounds and scars formed ex vivo to show that fascia fibroblasts upregulate N-cadherin allowing coordinated cell migration that drives extensive scar formation of deep wounds.
- Dongsheng Jiang
- , Simon Christ
- & Yuval Rinkevich
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Article
| Open Access
Direct observation of independently moving replisomes in Escherichia coli
How chromosome replication and segregation is organised in E. coli is a matter of debate. Here the authors visualise the bacterial chromosome and the replisomes during DNA replication, providing support for a previously suggested train track model.
- Aleksandre Japaridze
- , Christos Gogou
- & Cees Dekker
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Article
| Open Access
Adaptive prospective optical gating enables day-long 3D time-lapse imaging of the beating embryonic zebrafish heart
Imaging heart development is challenging due to constant tissue movement and changing physical landmarks. Here the authors present an algorithm capable of maintaining phase-locked imaging throughout a 24 hour timespan, enabling long term timelapse imaging studies of zebrafish heart development, repair and regeneration.
- Jonathan M. Taylor
- , Carl J. Nelson
- & Martin A. Denvir
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Article
| Open Access
Live imaging screen reveals that TYRO3 and GAK ensure accurate spindle positioning in human cells
Correct spindle positioning is critical for cell division but the full set of proteins regulating this is unclear. Here, with a live imaging siRNA-based screen in human cells, the authors identify 16 candidates required for this process, 11 of which were previously unassociated with such a function, including TYRO3 and GAK.
- Benita Wolf
- , Coralie Busso
- & Pierre Gönczy
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Article
| Open Access
Long-term optical brain imaging in live adult fruit flies
Time-lapse imaging studies of more than a day in the fly brain have been infeasible until now. Here the authors present a laser microsurgery approach to create a permanent window in the fly cuticle to enable time-lapse imaging of neural architecture and dynamics for up to 10–50 days.
- Cheng Huang
- , Jessica R. Maxey
- & Mark J. Schnitzer
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| Open Access
Functional organization of cytoplasmic inclusion bodies in cells infected by respiratory syncytial virus
Respiratory syncytial virus (RSV) induces formation of inclusion bodies (IBs) sheltering viral RNA synthesis. Here, Rincheval et al. identify highly dynamic IB-associated granules (IBAGs) that accumulate newly synthetized viral mRNA and the viral M2-1 protein but exclude viral genomic RNA and RNA polymerase complexes.
- Vincent Rincheval
- , Mickael Lelek
- & Marie-Anne Rameix-Welti
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Article
| Open Access
Spatiotemporal dynamics of lesion-induced axonal sprouting and its relation to functional architecture of the cerebellum
Neurodegenerative lesions induce sprouting from surviving axons, but the patterns of re-innervation of these collaterals in relation to existing functional networks remains unclear. Here the authors performed long term in vivoimaging in mice, of sprouts from cerebellar climbing fibers after a lesion, and describe the patterns of connectivity relative to functionally active zones.
- Matasha Dhar
- , Joshua M. Brenner
- & Hiroshi Nishiyama
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Article
| Open Access
Long-term time-lapse microscopy of C. elegans post-embryonic development
Long-term time-lapse imaging of motile developing organisms at the single cell level is challenging. Here the authors culture free-moving C. eleganslarvae in hydrogel chambers containing a food source, and use fast image acquisition to follow developmental processes.
- Nicola Gritti
- , Simone Kienle
- & Jeroen Sebastiaan van Zon
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Article
| Open Access
Dynamic blastomere behaviour reflects human embryo ploidy by the four-cell stage
Abnormal human embryo development is implicated in the embryo arrest observed during in vitrofertilization. Chavez and colleagues perform time-lapse imaging on human embryos and find that chromosomally abnormal embryos exhibit diverse cell cycle parameters that may contribute to arrest.
- Shawn L. Chavez
- , Kevin E. Loewke
- & Renee A. Reijo Pera
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Subcellular in vivo time-lapse imaging and optical manipulation of Caenorhabditis elegans in standard multiwell plates
The nematodeCaenorhabditis elegans is a widely used model organism for visualizing development, ageing and other phenomena, but high-throughput imaging requires immobilization of the animal. Rohde and Yanik report a rapid immobilization and imaging method for the high-throughput screening of C. elegans.
- Christopher B. Rohde
- & Mehmet Fatih Yanik
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Development and host cell modifications of Plasmodium falciparum blood stages in four dimensions
ThePlasmodium falciparum parasite that causes malaria has a complex life cycle in human erythrocytes. Using time-lapse three-dimensional imaging, the authors show the intraerythrocytic stages of the parasite and provide new insight into the export of P. falciparumproteins to Maurer's clefts.
- Christof Grüring
- , Arlett Heiber
- & Tobias Spielmann
Spatial and functional arrangement of Ebola virus polymerase inside phase-separated viral factories