Reverse transcription polymerase chain reaction articles within Nature Communications

Featured

• Article
  03 February 2022 | Open Access

  A disease-linked lncRNA mutation in RNase MRP inhibits ribosome synthesis

  Mutations in the non-coding RNA RMRP cause primary immunodeficiency. Robertson et al show that a disease-associated mutation in RMRP impairs pre-ribosomal RNA processing and reduces ribosome abundance, establishing this disorder as a ribosomopathy.

  • Nic Robertson
  • , Vadim Shchepachev
  •  & David Tollervey

• Article
  01 October 2021 | Open Access

  Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA

  While the analysis of SARS-CoV-2 RNA in stool samples has led to important insights regarding the disease, quantification is currently challenging. Here the authors use patient samples to benchmark preservation, extraction and quantification methods to optimise detection of viral RNA.

  • Aravind Natarajan
  • , Alvin Han
  •  & Ami S. Bhatt

• Article
  20 July 2021 | Open Access

  High-depth spatial transcriptome analysis by photo-isolation chemistry

  Spatial analysis of RNAseq data is important. Here the authors report a method for transcriptome profiling combined with photo-isolation chemistry to allow determination of expression profiles specifically from photo-irradiated regions of interest which they use in mouse brains and embryonic tissues.

  • Mizuki Honda
  • , Shinya Oki
  •  & Yasuyuki Ohkawa

• Article
  19 June 2020 | Open Access

  Substrate specificity of the TRAMP nuclear surveillance complexes

  During nuclear surveillance in yeast and human cells, the RNA exosome functions together with the TRAMP complexes. Here, the authors defined the protein composition of the TRAMP complexes and identified specific RNA binding sites for the different TRAMP components.

  • Clémentine Delan-Forino
  • , Christos Spanos
  •  & David Tollervey

• Article
  10 June 2020 | Open Access

  A multi-omics analysis reveals the unfolded protein response regulon and stress-induced resistance to folate-based antimetabolites

  The unfolded protein response (UPR) is a stress response pathway implicated in numerous diseases and chemotherapy resistance. Here, the authors define the UPR regulon with a multi-omics strategy, uncovering changes to mitochondrial one-carbon metabolism and concomitant resistance to folate-based therapeutics.

  • Stefan Reich
  • , Chi D. L. Nguyen
  •  & Jan Medenbach

• Article
  16 June 2017 | Open Access

  Single-cell profiling reveals heterogeneity and functional patterning of GPCR expression in the vascular system

  GPCRs are key regulators of vascular functions. By analysing single-cell GPCRs expression in vascular smooth muscle and endothelial cells from healthy and diseased murine vessels, Kauret al. show that GPCR expression is highly heterogeneous in all cell types and that disease causes GPCR repertoire changes depending on cell type and vascular localization.

  • H. Kaur
  • , J. Carvalho
  •  & N. Wettschureck