Protein purification articles within Nature Communications

Featured

  • Article
    | Open Access

    Epitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super-resolution imaging and in vivo detection of proteins.

    • Hansjörg Götzke
    • , Markus Kilisch
    •  & Steffen Frey

  • Article
    | Open Access

    The properties of many transmembrane or aggregation-prone proteins make them difficult to recombinantly express. Here the authors use a modified N-terminal domain of a spider silk protein to express and purify several difficult to express proteins at levels considerably higher than with conventional tags.

    • Nina Kronqvist
    • , Médoune Sarr
    •  & Jan Johansson

  • Article
    | Open Access

    A large portion of the proteome carries out its cellular function as part of macromolecular complexes. Here the authors describe Virotrap, a novel lysis-free approach for the isolation and identification of biologically relevant protein-protein and small molecule-protein interactions.

    • Sven Eyckerman
    • , Kevin Titeca
    •  & Jan Tavernier

  • Article
    | Open Access

    RNA-binding proteins are involved in the posttranscriptional regulation of a large number of cellular processes and several recent studies have sought to describe the extent of the RNA-binding proteome. Here, Conrad et al. describe serIC, a stringent approach they apply towards defining the RNA-binding proteome of the mammalian nucleus.

    • Thomas Conrad
    • , Anne-Susann Albrecht
    •  & Ulf Andersson Ørom

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