Article
|
Open Access
Featured
-
-
Article
| Open AccessDynamic tracking and identification of tissue-specific secretory proteins in the circulation of live mice
The in vivo identification of proteins secreted from a specific cell type or tissue remains challenging. Here, the authors develop a proximity labeling-based method to selectively label secreted proteins and combine it with proteomics to identify liver secretory proteins in mouse plasma.
- Kwang-eun Kim
- , Isaac Park
- & Jae Myoung Suh
-
Article
| Open AccessClassification of mouse B cell types using surfaceome proteotype maps
Analysis of the cell surface proteome (surfaceome) is essential for cell classification but is technically challenging. Here the authors miniaturize and automate the Cell Surface Capture method to increase sensitivity, reproducibility and throughput, and use it to create population-specific surfaceome maps of developing mouse B cells.
- Marc van Oostrum
- , Maik Müller
- & Bernd Wollscheid
-
Article
| Open AccessAn enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins
Understanding the functions of protein glycosylation critically depends on methods to efficiently enrich glycoproteins from complex samples. Here, the authors develop a strategy using dendrimer-conjugated benzoboroxole to enhance glycopeptide enrichment, providing the basis for more comprehensive glycoprotein analyses.
- Haopeng Xiao
- , Weixuan Chen
- & Ronghu Wu
-
Article
| Open AccessHydrogen bond based smart polymer for highly selective and tunable capture of multiply phosphorylated peptides
Capture of low-abundance multiply phosphorylated peptides (MPPs) is difficult due to limitation of enrichment materials and their interactions with phosphates. Here the authors show, a smart polymer driven by specific but tunable hydrogen bonding interactions can differentially complex with MPPs, singly phosphorylated and non-modified peptides.
- Guangyan Qing
- , Qi Lu
- & Taolei Sun