Origin firing articles within Nature

Featured

• Article | 05 April 2023

  Establishment and function of chromatin organization at replication origins

  Genome-scale in vitro reconstitution of DNA replication through chromatin establishes a crucial role for the origin recognition complex in organizing nucleosome arrays that are crucial for the initiation of replication.

  • Erika Chacin
  • , Karl-Uwe Reusswig
  •  & Christoph F. Kurat

• Article
  15 June 2022 | Open Access

  Mechanism of replication origin melting nucleated by CMG helicase assembly

  Cryo-electron microscopy structures of the sequential assembly of the CMG replicative helicase on a chromatinized origin of replication provide insights into the mechanism through which DNA melting is initiated by ATP binding.

  • Jacob S. Lewis
  • , Marta H. Gross
  •  & Alessandro Costa

• Article | 04 May 2022

  CDC7-independent G1/S transition revealed by targeted protein degradation

  In addition to CDC7, the cell cycle kinase CDK1 has a pivotal role in the G1/S transition of cells, a finding that revises our current understanding of cell cycle progression.

  • Jan M. Suski
  • , Nalin Ratnayeke
  •  & Piotr Sicinski

• Article | 21 October 2020

  Equilibrium between nascent and parental MCM proteins protects replicating genomes

  Mother cells recycle parental MCMs and simultaneously synthesize nascent MCMs, both of which are inherited by daughter cells, in which the former are preferentially used to form active replisomes and the latter adjust the pace of replisome movement to minimize errors during DNA replication.

  • Hana Sedlackova
  • , Maj-Britt Rask
  •  & Jiri Lukas

• Letter | 28 February 2018

  The mechanism of eukaryotic CMG helicase activation

  In vitro experiments, using purified proteins and an assay that detects DNA unwinding, reveal the mechanism of activation of eukaryotic DNA replication.

  • Max E. Douglas
  • , Ferdos Abid Ali
  •  & John F. X. Diffley

• Letter | 08 June 2016

  The bacterial DnaA-trio replication origin element specifies single-stranded DNA initiator binding

  The bacterial chromosome replication origin contains an indispensable element composed of a repeating trinucleotide motif, termed the DnaA-trio, that stabilizes DnaA binding on single-stranded DNA.

  • Tomas T. Richardson
  • , Omar Harran
  •  & Heath Murray

• Article | 29 July 2015

  Structure of the eukaryotic MCM complex at 3.8 Å

  Cryo-electron microscopy is used to visualize the double hexamer of the eukaryotic minichromosome maintenance complex (MCM), which is assembled during the G1 phase of DNA replication; two interdigitated hexamers have a central channel that tightly fits a DNA duplex, and the orientation of the tilted single hexamers sheds light on many functional aspects, particularly in the initial origin DNA melting.

  • Ningning Li
  • , Yuanliang Zhai
  •  & Ning Gao

• Letter | 03 November 2013

  Accelerated growth in the absence of DNA replication origins

  When all origins of replication are deleted from the archaeon Haloferax volcanii, homologous recombination is used to initiate DNA replication and the growth rate is accelerated.

  • Michelle Hawkins
  • , Sunir Malla
  •  & Thorsten Allers

• Letter | 28 July 2013

  Avoiding chromosome pathology when replication forks collide

  The site of collision between two chromosome replication forks can be used to reinitiate replication independent of an active origin, with potentially pathogenic effects.

  • Christian J. Rudolph
  • , Amy L. Upton
  •  & Robert G. Lloyd