Article
|
Open Access
Featured
-
-
Article
| Open AccessAutomated optogenetic feedback control for precise and robust regulation of gene expression and cell growth
Optogenetics has emerged as a promising means to achieve gene expression control in bioprocess engineering, but current systems cannot respond to fluctuations in growth conditions. Here the authors overcome this limitation and develop an automated optogenetic feedback control system for precise and robust control of protein production in E. coli.
- Andreas Milias-Argeitis
- , Marc Rullan
- & Mustafa Khammash
-
Article
| Open AccessPhototriggered protein syntheses by using (7-diethylaminocoumarin-4-yl)methoxycarbonyl-caged aminoacyl tRNAs
Spatiotemporal regulation of protein synthesis would advance studies into the consequences of localised protein translation in cells and tissues. Here, Ohtsuki et al.improve on an earlier caged-tRNA design to provide caged aminoacyl-tRNAs that are rapidly uncaged by visible light.
- Takashi Ohtsuki
- , Shigeto Kanzaki
- & Kazunori Watanabe
-
Article
| Open AccessOptogenetic dissection of ictal propagation in the hippocampal–entorhinal cortex structures
The network mechanism supporting seizure spread in temporal lobe epilepsy (TLE) is only partially understood. Using optogenetic methods, Lu et al.identify a feed-forward propagation pathway of ictal discharges from the dentate gyrus/hilus to the medial entorhinal cortex in a mouse model of TLE.
- Yi Lu
- , Cheng Zhong
- & Liping Wang
-
Article
| Open AccessOptogenetic control of nuclear protein export
Light-inducible control of protein subcellular localization holds great promise for synthetic biology applications and insights into basic cell biology. Here the authors develop a genetically-encoded light-inducible nuclear export system and apply it to a synthetic repressor and p53 transcriptional activity.
- Dominik Niopek
- , Pierre Wehler
- & Barbara Di Ventura
-
Article
| Open AccessOptogenetic mutagenesis in Caenorhabditis elegans
Inducing random mutation of C. elegansDNA is a widely used technique to investigate gene and protein function. Here the authors introduce a method of optogenetic mutagenesis, driving the generation of reactive oxygen species, which avoids the use of toxic chemicals.
- Kentaro Noma
- & Yishi Jin
-
Article
| Open AccessNeuronal and behavioural modulations by pathway-selective optogenetic stimulation of the primate oculomotor system
Pathway-selective optogenetics enables the precise control of neural activity in targeted pathways connecting specific brain regions. Here the authors provide the first demonstration that pathway-selective optogenetics can modulate neural activity and behaviour in non-human primates.
- Ken-ichi Inoue
- , Masahiko Takada
- & Masayuki Matsumoto
-
Article
| Open AccessSonogenetics is a non-invasive approach to activating neurons in Caenorhabditis elegans
Common optogenetic approaches require surgical procedures to deliver light of specific wavelengths to the target cells. Here the authors demonstrate the use of low-pressure ultrasound as a non-invasive trigger to activate specific neurons in Caenorhabditis elegansand find that the mechanotransduction channel TRP-4 sensitizes cells to the ultrasound stimulus.
- Stuart Ibsen
- , Ada Tong
- & Sreekanth H. Chalasani
-
Article
| Open AccessOptogenetic manipulation of cGMP in cells and animals by the tightly light-regulated guanylyl-cyclase opsin CyclOp
Cyclic guanosine monophosphate (cGMP) is a cellular second messenger involved in many processes including regulation of neuronal excitability and vascular tone. Gao, Nagpalet al., employ a fungal rhodopsin to optogenetically control cGMP levels in multiple systems including C. eleganssensory neurons.
- Shiqiang Gao
- , Jatin Nagpal
- & Alexander Gottschalk
-
Article
| Open AccessLight generation of intracellular Ca2+ signals by a genetically encoded protein BACCS
Current tools for optogenetic control of intracellular calcium signals currently suffer from slow response time or low dynamic range. Here the authors develop blue light-activated Ca2+ channel switch (BACCS) that modulates the activity of Ca2+-sensitive Orai channels with high temporal resolution and large dynamic range.
- Tomohiro Ishii
- , Koji Sato
- & Takao Nakata
-
Article
| Open AccessOptogenetic control of contractile function in skeletal muscle
Nerve damage can lead to skeletal muscle paralysis. The authors show that localized photostimulation of skeletal muscle expressing the light-sensitive channel Channelrhodopsin-2 generates contraction in the absence of neural impulses and prove that this strategy can be used for optogenetic opening of explanted murine vocal cords.
- Tobias Bruegmann
- , Tobias van Bremen
- & Philipp Sasse
-
Article
| Open AccessAtomistic design of microbial opsin-based blue-shifted optogenetics tools
Retinal-bound opsins are widely used tools for optical control of neuronal activity in vivo, so called optogenetics. Here, using molecular simulations, biochemistry, electrophysiology and X-ray crystallography, the authors present new molecular design principles for the generation of blue-shifted variants of microbial rhodopsins.
- Hideaki E. Kato
- , Motoshi Kamiya
- & Osamu Nureki
-
Article |
Regulation of endogenous transmembrane receptors through optogenetic Cry2 clustering
Signaling through transmembrane receptors regulates diverse biological processes including cell proliferation, motility and differentiation. Here, the authors demonstrate the optogenetic control of endogenous transmembrane receptor activity through clustering using a new modular strategy.
- L. J. Bugaj
- , D. P. Spelke
- & D. V. Schaffer
-
Article |
Visually induced initiation of Drosophila innate courtship-like following pursuit is mediated by central excitatory state
Male Drosophilaexhibit a complex suite of courtship behaviours in response to a variety of sensory stimuli. Here, the authors demonstrate the involvement of specific, genetically defined clusters of central neurons in visually induced initiation and maintenance of courtship behaviours.
- Soh Kohatsu
- & Daisuke Yamamoto
-
Article |
Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins
Photoreceptor-based photoswitches have proved to be powerful tools for the specific control of protein activity in live cells. Here the authors describe Magnets, a new set of photoswitches based on the Vivid photoreceptor with enhanced hetero-dimerization specificity and variable activation kinetics.
- Fuun Kawano
- , Hideyuki Suzuki
- & Moritoshi Sato
-
Article
| Open AccessAntagonistic interplay between hypocretin and leptin in the lateral hypothalamus regulates stress responses
The hypothalamic-pituitary-adrenal axis coordinates behavioral and physiological responses to stress but the mechanisms are poorly understood. Here, the authors show that neurons that produce hypocretin/orexin in the lateral hypothalamic area regulate corticosterone release and a variety of behaviors related to the stress response.
- Patricia Bonnavion
- , Alexander C. Jackson
- & Luis de Lecea
-
Article |
Synthetic retinal analogues modify the spectral and kinetic characteristics of microbial rhodopsin optogenetic tools
Efforts to improve the performance of optogenetic tools for neuroscience research have mostly been focused on mutating the opsin backbones or mining-related algal genomes. Here the authors show that analogues of the chromophore, retinal, can be used for colour tuning of rhodopsins and altering their photocycle kinetics in several model organisms.
- N. AzimiHashemi
- , K. Erbguth
- & J. F. Liewald
-
Article
| Open AccessMind-controlled transgene expression by a wireless-powered optogenetic designer cell implant
Brain–machine interfaces offer the possibility of controlling prosthetic devices using changes in brain activity. Folcher et al.couple such a system wirelessly to an optogenetic implant in mice to control expression of a transgene, demonstrating its potential for mind-controlled drug delivery.
- Marc Folcher
- , Sabine Oesterle
- & Martin Fussenegger
-
Article
| Open AccessGraphene-based carbon-layered electrode array technology for neural imaging and optogenetic applications
Monitoring neuronal activity in the rodent in vivobrain is commonly done using micro-electrode arrays but these devices are not normally compatible with optical technologies. Here the authors design a transparent and flexible electrode array based on graphene that allows them to combine electrophysiological recordings with optogenetic and imaging experiments.
- Dong-Wook Park
- , Amelia A. Schendel
- & Justin C. Williams
-
Article |
An optimized optogenetic clustering tool for probing protein interaction and function
Protein–protein interactions are fundamental to nearly all molecular and cellular processes. Here Taslimi et al.describe a versatile new optogenetic module that can be used to visualize protein–protein interactions, as well as reversibly control them with light with spatiotemporal resolution.
- Amir Taslimi
- , Justin D. Vrana
- & Chandra L. Tucker
-
Article |
A comprehensive multiscale framework for simulating optogenetics in the heart
Optogenetics can be used to induce an electrical response in cardiac cells and could become an alternative to electrical cardiac pacing. Here, Boyleet al.present a modelling framework for cardiac optogenetics and explore how optogenetic stimulation might be used to alter cardiac function.
- Patrick M. Boyle
- , John C. Williams
- & Natalia A. Trayanova
-
Article |
Ultraviolet-B-mediated induction of protein–protein interactions in mammalian cells
Light-sensitive proteins are useful tools to control protein localization and gene expression, but are currently limited to excitation with red or blue light. Here Crefcoeur et al. present a novel optogenetic system to induce protein–protein interactions with ultraviolet-B light that does not require exogenous chromophores.
- Remco P. Crefcoeur
- , Ruohe Yin
- & Thanos D. Halazonetis
-
Article
| Open AccessOn-demand optogenetic control of spontaneous seizures in temporal lobe epilepsy
Temporal lobe epilepsy in adults does not always respond to treatment. Krook-Magnuson and colleagues use optogenetics to inhibit and activate excitatory and inhibitory neurons, respectively, in a mouse model of temporal lobe epilepsy, and find that they can stop seizures on a moment-to-moment basis.
- Esther Krook-Magnuson
- , Caren Armstrong
- & Ivan Soltesz
-
Article |
Nanodomain Ca2+ of Ca2+ channels detected by a tethered genetically encoded Ca2+ sensor
Calcium nanodomains arise from the cytoplasmic mouth of calcium channels but have not been directly visualized. In this study, the nanodomain of the CaV2.2 calcium channel is imaged using total internal reflection fluorescence microscopy and a genetically encoded calcium sensor attached to the carboxy terminus.
- Lai Hock Tay
- , Ivy E. Dick
- & David T. Yue