Oligonucleotide probes articles within Nature Communications

Featured

• Article
  22 May 2023 | Open Access

  A smart pathogen detector engineered from intracellular hydrogelation of DNA-decorated macrophages

  Macrophages have the inherent capacity to recognise pathogenic microorganisms, highlighting their potential to be used in biosensing applications. Herein, the authors report a method to transform normally fragile macrophages into robust gelated cell particles for efficient bacterial capture and detection.

  • Yueyue Gui
  • , Yujing Zeng
  •  & Chao Li

• Article
  05 November 2022 | Open Access

  FRET-FISH probes chromatin compaction at individual genomic loci in single cells

  Chromatin compaction affects many nuclear processes yet compaction levels at individual genomic loci have been notoriously difficult to assess. Here, Ana Mota and co-authors from the Bienko-Crosetto Lab present FRET-FISH for probing chromatin compaction at selected loci in single cells.

  • Ana Mota
  • , Szymon Berezicki
  •  & Magda Bienko

• Article
  05 February 2021 | Open Access

  Rapid electrochemical detection of coronavirus SARS-CoV-2

  Currently the most common method of COVID-19 diagnosis is by qRT-PCR which is slow and requires expensive instrumentation. Here the authors report an electrochemical biosensor based on isothermal rolling circle amplification for rapid detection of SARS-CoV-2 in clinical samples.

  • Thanyarat Chaibun
  • , Jiratchaya Puenpa
  •  & Benchaporn Lertanantawong

• Article
  01 December 2020 | Open Access

  Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood

  Reliable plasmonic biosensors with high throughput and ease of use are highly sought after. Here, the authors report a plasmon-enhanced fluorescence antibody-aptamer biosensor based on a gold nanoparticle array, and demonstrate its use for effective specific detection of a malaria marker, at femtomolar level, in whole blood.

  • Antonio Minopoli
  • , Bartolomeo Della Ventura
  •  & Raffaele Velotta

• Article
  09 April 2019 | Open Access

  iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture

  DNA FISH allows for the visual analysis of chromosomal organisation in individual cells. Here the authors present iFISH, an open-source repository of ready-to-use DNA FISH probes along with tools for probe design.

  • Eleni Gelali
  • , Gabriele Girelli
  •  & Magda Bienko

• Article
  20 February 2019 | Open Access

  Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader

  Assessment of luteinising hormone pulsatility is important in the diagnosis of reproductive disorders. Here the authors develop a DNA aptamer-based electrochemical analysis integrated into a robotic platform for high-throughput and sensitive analysis.

  • Shaolin Liang
  • , Andrew B. Kinghorn
  •  & Julian A. Tanner

• Article
  14 March 2017 | Open Access

  Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome

  Analysis of virus replication on a single-cell level is often hampered by a lack of specific or sensitive enough reagents. Here, Douamet al. use RNA-flow technique to track (+) and (−) strand RNA of yellow fever virus in hematopoietic cells in mouse models and identify virus-host interactions that affect disease outcome.

  • Florian Douam
  • , Gabriela Hrebikova
  •  & Alexander Ploss

• Article
  16 November 2015 | Open Access

  Scalable amplification of strand subsets from chip-synthesized oligonucleotide libraries

  Synthetic oligonucleotides are the main cost factor for studies in DNA nanotechnology. Here, the authors present a selective oligonucleotide amplification method, based on three rounds of rolling-circle amplification, that produces nanomole amounts of single-stranded oligonucleotides per millilitre reaction.

  • Thorsten L. Schmidt
  • , Brian J. Beliveau
  •  & William M. Shih