Model prokaryotes articles within Nature Communications

Featured

• Article
  14 February 2024 | Open Access

  Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography

  Bacterial endospores are among the most resilient forms of life. Here, authors reveal ultrastructural details of the spore chromosome and the multiprotein, multilayered extracellular coat, shedding light on mechanisms contributing to spore resistance.

  • Elda Bauda
  • , Benoit Gallet
  •  & Cecile Morlot

• Article
  26 March 2021 | Open Access

  The Burkholderia pseudomallei intracellular ‘TRANSITome’

  Prokaryotic cell transcriptomics has been limited to mixed or sub-population dynamics and individual cells within heterogeneous populations. Here the authors develop a ‘TRANSITomic’ approach to profile transcriptomes of single Burkholderia pseudomallei cells as they transit through host cell infection.

  • Yun Heacock-Kang
  • , Ian A. McMillan
  •  & Tung T. Hoang

• Article
  11 December 2020 | Open Access

  Machine learning uncovers independently regulated modules in the Bacillus subtilis transcriptome

  The systems-level regulatory structure underlying gene expression in bacteria can be inferred using machine learning algorithms. Here we show this structure for Bacillus subtilis, present five hypotheses gleaned from it, and analyse the process of sporulation from its perspective.

  • Kevin Rychel
  • , Anand V. Sastry
  •  & Bernhard O. Palsson

• Article
  20 March 2020 | Open Access

  Single-molecule live-cell imaging visualizes parallel pathways of prokaryotic nucleotide excision repair

  In Escherichia coli, the UvrAB damage sensor recognizes helix-distorting lesions by itself or via Mfd bound to stalled RNA polymerase. Here authors use single-molecule fluorescence imaging to quantify the kinetic signatures of interactions of UvrA with Mfd and UvrB in live cells.

  • Harshad Ghodke
  • , Han Ngoc Ho
  •  & Antoine M. van Oijen