Featured
-
-
Letter |
Genomic profiling of DNA methyltransferases reveals a role for DNMT3B in genic methylation
Genome-wide localization and activity analysis of the de novo DNA methyltransferases DNMT3A and DNMT3B in mouse embryonic stem cells identifies overlapping and individual targeting preferences to the genome, including a role for DNMT3B in gene body methylation.
- Tuncay Baubec
- , Daniele F. Colombo
- & Dirk Schübeler
-
Letter |
Cysteine methylation disrupts ubiquitin-chain sensing in NF-κB activation
A conserved protein from enteropathogenic Escherichia coli, NleE, inhibits innate immune defence against infection by disrupting the NF-κB signalling pathway through methylation of ubiquitin-chain sensing proteins.
- Li Zhang
- , Xiaojun Ding
- & Feng Shao
-
Letter |
MMSET regulates histone H4K20 methylation and 53BP1 accumulation at DNA damage sites
Recruitment of 53BP1 to double-strand DNA breaks is an important step in the cellular response to DNA damage. Here, the histone methyltransferase MMSET is shown to be responsible for localized increases in a histone modification that is involved in recruiting 53BP1. The mechanism of MMSET recruitment to DNA damage sites is also investigated.
- Huadong Pei
- , Lindsey Zhang
- & Zhenkun Lou
-
Letter |
A methyl transferase links the circadian clock to the regulation of alternative splicing
Various biological processes are entrained by the day–night cycle to occur at a specific time of day. One way the circadian system exerts these effects is through post-transcriptional regulation. These authors show that a protein that transfers methyl groups onto several spliceosome subunits, PRMT5, is regulated by the light–dark cycle. Methylation of these subunits affects alternative splicing of some genes, thus making them subject to circadian control.
- Sabrina E. Sanchez
- , Ezequiel Petrillo
- & Marcelo J. Yanovsky
-
Letter |
NRMT is an α-N-methyltransferase that methylates RCC1 and retinoblastoma protein
α-N-methylation is an unusual post-translational modification in which the amino-terminal residues of proteins are methylated. One example is the Ran guanine nucleotide-exchange factor, RCC1, which requires methylation for its association with chromatin. These authors describe the first α-N-methyltransferase, named N-terminal RCC1 methyltransferase (NRMT). They identify the NRMT recognition sequence and several new methylation targets, and demonstrate the importance of α-N-methylation for normal bipolar spindle formation and chromosome segregation.
- Christine E. Schaner Tooley
- , Janusz J. Petkowski
- & Ian G. Macara