Featured
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Periodic inhibition of Erk activity drives sequential somite segmentation
The zebrafish segmentation clock drives sequential segmentation of somites by periodically lowering double-phosphorylated Erk and therefore projecting its oscillation on the double-phosphorylated Erk gradient.
- M. Fethullah Simsek
- , Angad Singh Chandel
- & Ertuğrul M. Özbudak
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Single-cell transcriptomic characterization of a gastrulating human embryo
The single-cell transcriptional profile of a human embryo between 16 and 19 days after fertilization reveals parallels and differences in gastrulation in humans as compared with mouse and non-human primate models.
- Richard C. V. Tyser
- , Elmir Mahammadov
- & Shankar Srinivas
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Single-cell and spatial transcriptomics reveal somitogenesis in gastruloids
Single-cell RNA sequencing and spatial transcriptomics reveal that the somitogenesis clock is active in mouse gastruloids, which can be induced to generate somites with the correct rostral–caudal patterning.
- Susanne C. van den Brink
- , Anna Alemany
- & Alexander van Oudenaarden
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In vitro characterization of the human segmentation clock
Human presomitic mesoderm cells derived in vitro demonstrate oscillations of the segmentation clock, thus providing a window into an otherwise inaccessible stage of human development.
- Margarete Diaz-Cuadros
- , Daniel E. Wagner
- & Olivier Pourquié
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Article |
Coupling delay controls synchronized oscillation in the segmentation clock
Monitoring cells of the mouse presomitic mesoderm using the Achilles reporter fused to HES7 sheds light on the mechanisms that underpin synchronous oscillations in the expression of clock genes between neighbouring cells.
- Kumiko Yoshioka-Kobayashi
- , Marina Matsumiya
- & Ryoichiro Kageyama
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Letter |
Scaling of embryonic patterning based on phase-gradient encoding
An ex vivo primary culture assay is developed that recapitulates mouse embryonic mesodermal patterning and segment formation; using this approach, it is shown that oscillating gene activity is central to maintain stable proportions during development.
- Volker M. Lauschke
- , Charisios D. Tsiairis
- & Alexander Aulehla
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Letter |
Pericytes regulate the blood–brain barrier
The blood–brain barrier (BBB) is made up of vascular endothelial cells and was thought to have formed postnatally from astrocytes. Two independent studies demonstrate that this barrier forms during embryogenesis, with pericyte/endothelial cell interactions being critical to regulate the BBB during development. A better understanding of the relationship among pericytes, neuroendothelial cells and astrocytes in BBB function will contribute to our understanding of BBB breakdown during central nervous system injury and disease.
- Annika Armulik
- , Guillem Genové
- & Christer Betsholtz
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Letter |
Rere controls retinoic acid signalling and somite bilateral symmetry
The vertebrate body plan shows marked bilateral symmetry, although this can be disrupted in conditions such as scoliosis. Here, a mutation in Rere is found that leads to the formation of asymmetrical somites in mouse embryos; furthermore, Rere is shown to control retinoic acid signalling, which is required to maintain somite symmetry by interacting with Fgf8. The results provide insight into how bilateral symmetry is maintained.
- Gonçalo C. Vilhais-Neto
- , Mitsuji Maruhashi
- & Olivier Pourquié