Membrane proteins articles within Nature

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  • Article
    | Open Access

    Structural analysis of the human choline and ethanolamine transporters FLVCR1 and FLVCR2 clarifies the mechanisms of transport, the conformational dynamics of these proteins and the disease-associated mutations that interfere with these processes.

    • Keiken Ri
    • , Tsai-Hsuan Weng
    •  & Schara Safarian

  • Article |

    Monoamines and neurotoxicants share a binding pocket in VMAT1 featuring polar sites for specificity and a wrist-and-fist shape for versatility, and monoamine enrichment in storage vesicles arises from dominant import via favoured lumenal-open transition of VMAT1 and protonation-precluded binding during its cytoplasmic-open transition.

    • Jin Ye
    • , Huaping Chen
    •  & Weikai Li

  • Article |

    Time-resolved cryo-EM is used to capture structural transitions during G-protein activation stimulated by a G-protein-coupled receptor.

    • Makaía M. Papasergi-Scott
    • , Guillermo Pérez-Hernández
    •  & Georgios Skiniotis

  • Article
    | Open Access

    The authors introduce a single-molecule DNA-barcoding method, resolution enhancement by sequential imaging, that improves the resolution of fluorescence microscopy down to the Ångström scale using off-the-shelf fluorescence microscopy hardware and reagents.

    • Susanne C. M. Reinhardt
    • , Luciano A. Masullo
    •  & Ralf Jungmann

  • Article |

    Combining genome-wide CRISPR screens with massively parallel analyses of human and random DNA sequences reveal a unified mechanism for the surveillance and evolution of translation products from annotated noncoding DNA.

    • Jordan S. Kesner
    • , Ziheng Chen
    •  & Xuebing Wu

  • Article
    | Open Access

    A structure–function analysis of cystic fibrosis transmembrane conductance regulator shows its two nucleotide-binding domains dimerize before channel opening, and reveals a mechanism through which conformational changes in the channel regulate chloride conductance.

    • Jesper Levring
    • , Daniel S. Terry
    •  & Jue Chen

  • Article |

    A cryo-electron microscopy analysis of the Chlamydomonas reinhardtii TOC–TIC supercomplex reveals that Tic214 traverses the chloroplast inner membrane, the intermembrane space and the outer membrane, connecting the TOC complex with the TIC proteins.

    • Hao Liu
    • , Anjie Li
    •  & Zhenfeng Liu

  • Article |

    Two cyclic dinucleotide molecules bind within the cavity of human SLC19A1 as a compact dual-molecule unit, whereas folate and antifolate bind to SLC19A1 as a monomer and occupy a distinct pocket of the cavity.

    • Qixiang Zhang
    • , Xuyuan Zhang
    •  & Pu Gao

  • Article
    | Open Access

    Structures of the Arabidopsis thaliana auxin exporter PIN1 in the apo state, bound to the natural auxin or bound to an inhibitor provide insights into the polar auxin transport mechanisms mediated by PIN family transporters.

    • Zhisen Yang
    • , Jing Xia
    •  & Linfeng Sun

  • Article |

    Structural determination of GAT1 using cryo-electron microscopy provides insights into the biology and pharmacology of this GABA transporter.

    • Zenia Motiwala
    • , Nanda Gowtham Aduri
    •  & Cornelius Gati

  • Article |

    Cryo-electron microscopy structures of the bacterial O-antigen ligase WaaL, combined with genetics, biochemistry and molecular dynamics simulations, provide insight into the mechanism by which WaaL catalyses the biosynthesis of lipopolysaccharide.

    • Khuram U. Ashraf
    • , Rie Nygaard
    •  & Filippo Mancia

  • Article |

    Cryo-EM structures of human calcium-sensing receptor reveal intrinsic asymmetry in the receptor homodimer upon activation that is stabilized by calcimimetic drugs adopting distinct poses in the two protomers, priming one protomer for G-protein coupling.

    • Yang Gao
    • , Michael J. Robertson
    •  & Georgios Skiniotis

  • Article
    | Open Access

    A cryo-electron microscopy structure of the inner membrane complex of the ESX-5 type VII secretion system of Mycobacterium tuberculosis reveals an important role of interactions with MycP5 protease for complex integrity.

    • Catalin M. Bunduc
    • , Dirk Fahrenkamp
    •  & Thomas C. Marlovits

  • Article |

    Cryo-electron microscopy structures of non-structural protein 1 (nsP1) of chikungunya virus reveal the mechanisms that underpin the association of viral replication machinery with virus-induced membranous organelles within host cells.

    • Rhian Jones
    • , Gabriel Bragagnolo
    •  & Juan Reguera

  • Article |

    An approach for the design of protein pores is demonstrated by the computational design and subsequent experimental expression of both an ion-selective and a large transmembrane pore.

    • Chunfu Xu
    • , Peilong Lu
    •  & David Baker

  • Article |

    Cryo-electron microscopy structures of heterodimeric and homodimeric full-length GABAB receptors, combined with cellular signalling assays, shed light on the mechanisms that underpin signal transduction mediated by these receptors.

    • Makaía M. Papasergi-Scott
    • , Michael J. Robertson
    •  & Georgios Skiniotis

  • Article |

    Cryo-electron microscopy structures of a folding intermediate on the BAM complex of Escherichia coli reveal how interactions between the BamA catalyst and substrate permit stable association during folding, followed by rapid turnover.

    • David Tomasek
    • , Shaun Rawson
    •  & Daniel Kahne

  • Article |

    Analyses reveal a previously undescribed transmembrane protein fold in the endoplasmic reticulum-based glucosyltransferase ALG6 and provide a structural basis for understanding the glucose transfer mechanism.

    • Joël S. Bloch
    • , Giorgio Pesciullesi
    •  & Kaspar P. Locher

  • Letter |

    Crystal structures of a five-protein complex comprising the inner-membrane components of the bacterial lipopolysaccharide transporter provide insight into the mechanism of extraction of lipopolysaccharide from the inner membrane and its transport to the outer membrane.

    • Tristan W. Owens
    • , Rebecca J. Taylor
    •  & Daniel Kahne

  • Article |

    Crystal structures of the gastric proton pump in complex with two inhibitory drugs reveal the mechanism that generates the steep acidic gradient across the membranes of parietal cells.

    • Kazuhiro Abe
    • , Katsumasa Irie
    •  & Yoshinori Fujiyoshi

  • Letter |

    The crystal structure of the bacterial potassium import complex KdpFABC shows how ATP hydrolysis is coupled to potassium transport to maintain cellular homeostasis under low potassium conditions.

    • Ching-Shin Huang
    • , Bjørn Panyella Pedersen
    •  & David L. Stokes

  • Article |

    The structure of human ABCG2 bound to an inhibitory antibody using cryo-electron microscopy, representing the first high-resolution structural data of a human multidrug transporter.

    • Nicholas M. I. Taylor
    • , Ioannis Manolaridis
    •  & Kaspar P. Locher

  • Article |

    High-resolution structures of the thermostabilized human excitatory amino acid transporter EAAT1, alone or in association with its substrate or small molecule inhibitors, reveal architectural features of human SLC1 transporters and an allosteric mechanism of inhibition.

    • Juan C. Canul-Tec
    • , Reda Assal
    •  & Nicolas Reyes

  • Review Article |

    This Review discusses current knowledge of the structure, function and interactions of the metabotropic glutamate and GABAB receptors and the potential to target receptor subunits for future therapeutic intervention in neurological and mental health disorders.

    • Jean-Philippe Pin
    •  & Bernhard Bettler

  • Article |

    Respirasomes are supercomplexes of mitochondrial electron transport chain complexes that are responsible for cellular respiration and energy production; cryo-electron microscopy structures of mammalian (sheep) respirasomes are presented.

    • James A. Letts
    • , Karol Fiedorczuk
    •  & Leonid A. Sazanov

  • Letter |

    The X-ray structure of the drug/metabolite transporter (DMT) protein YddG from Starkeya novella reveals a new membrane transport topology, with ten transmembrane segments in an outward-facing state and two pseudo-symmetric inverted structural repeats.

    • Hirotoshi Tsuchiya
    • , Shintaro Doki
    •  & Osamu Nureki

  • Article |

    X-ray crystal structures of the human serotonin transporter (SERT) bound to the antidepressants (S)-citalopram or paroxetine show that the antidepressants lock the protein in an outward-open conformation, and directly block serotonin from entering its binding site; the structures define the mechanism of antidepressant action in SERT and pave the way for future drug design.

    • Jonathan A. Coleman
    • , Evan M. Green
    •  & Eric Gouaux

  • Letter |

    The X-ray crystal structures of the human σ1 receptor bound to two different ligands are reported, revealing the overall architecture, oligomerization state, and molecular basis for ligand recognition by this protein.

    • Hayden R. Schmidt
    • , Sanduo Zheng
    •  & Andrew C. Kruse

  • Article |

    A high-resolution electron cryo-microscopy structure of the zebrafish α1 glycine receptor bound to agonists or antagonists reveals the conformational changes that take place when the channel transitions from closed to open state.

    • Juan Du
    • , Wei Lü
    •  & Eric Gouaux

  • Article |

    Gram-positive bacteria use peptidase-containing ATP-binding cassette transporters (PCATs) to export quorum-sensing and antimicrobial polypeptides; here, the X-ray crystal structures of PCAT1 from Clostridium thermocellum in the absence and presence of ATP are reported.

    • David Yin-wei Lin
    • , Shuo Huang
    •  & Jue Chen

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