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| Open AccessMultiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision
Single molecule force measurements have shed light on dynamic biological events, but rare events escape notice owing to low throughput of the methods. Here, the authors combine an array of magnetic tweezers with lateral flow to increase throughput 100-fold, and detect rare DNA breaks induced by gyrase.
- Rohit Agarwal
- & Karl E. Duderstadt
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Article
| Open AccessModulating mechanical stability of heterodimerization between engineered orthogonal helical domains
Mechanically stable specific heterodimerization formed with reversible bonds are used as a molecular anchorage in single-molecule force spectroscopy studies with unique mechanical properties. Here authors develop a variety of heterodimerization molecular systems with a range of mechanical stability from a set of recently engineered helix-heterotetramers.
- Miao Yu
- , Zhihai Zhao
- & Jie Yan
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Article
| Open AccessDirected manipulation of membrane proteins by fluorescent magnetic nanoparticles
Membrane proteins are embedded in the lipid bilayer of the plasma membrane and their function in this context is often linked to their specific location and dynamics within the membrane. Here authors report the use of fluorescent magnetic nanoparticles to track membrane molecules and to manipulate their movement and pull membrane components laterally through the membrane with femtonewton-range forces.
- Jia Hui Li
- , Paula Santos-Otte
- & Helge Ewers
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Article
| Open AccessDynamic and non-contact 3D sample rotation for microscopy
Sample orientation is crucial to ensure optimal image quality in light microscopy. Here the authors enable multi-axis orientation of fixed mouse embryos and shrimp, and live zebrafish embryos and larvae by introducing magnetic beads and rotating the sample with a magnetic field in a microscope.
- Frederic Berndt
- , Gopi Shah
- & Jan Huisken
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Article
| Open AccessFocused clamping of a single neuronal SNARE complex by complexin under high mechanical tension
The SNARE complex enables the fusion of synaptic vesicles with presynaptic membrane via a zippering process that is modulated by the protein complexin, though the precise mechanism remains unclear. Here, the authors used magnetic tweezers to show how complexin prepares a SNARE complex for fusion under mechanical tension.
- Min Ju Shon
- , Haesoo Kim
- & Tae-Young Yoon
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Camera-based three-dimensional real-time particle tracking at kHz rates and Ångström accuracy
Particle tracking with ultra-high resolution in optical and magnetic tweezers has so far relied on laser detection through photodiodes. Here, Huhle et al. demonstrate three-dimensional particle tracking with Ångström accuracy and real-time GPU-accelerated data processing at kHz rates using camera-based imaging.
- Alexander Huhle
- , Daniel Klaue
- & Ralf Seidel
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Article
| Open AccessFreely orbiting magnetic tweezers to directly monitor changes in the twist of nucleic acids
Rotational motion and torsional strain affects DNA replication, transcription and repair. Lipfertet al. have developed a new technique that uses freely orbiting magnetic tweezers to measure equilibrium fluctuations and determine the twist of tethered nucleic acid molecules.
- Jan Lipfert
- , Matthew Wiggin
- & Nynke H. Dekker