Featured
-
-
Article
| Open AccessInositol pyrophosphates activate the vacuolar transport chaperone complex in yeast by disrupting a homotypic SPX domain interaction
Pipercevic et al resolve how inositol molecules activate the VTC protein complex. The VTC complex stores phosphate in yeast and is controlled by SPX domains. The inositol molecules break an interaction between SPX domains to activate the complex.
- Joka Pipercevic
- , Bastian Kohl
- & Sebastian Hiller
-
Article
| Open AccessLarge-scale identification of genes involved in septal pore plugging in multicellular fungi
Multicellular fungi have septal pores that allow cytoplasmic exchange between adjacent cells; cell wounding and other stress conditions induce septal pore closure. Here, Mamun et al. determine the subcellular localization of hundreds of uncharacterized proteins in a multicellular fungus, identifying 62 proteins associated with the septum. Of these, 23 proteins are involved in septal pore plugging upon hyphal wounding.
- Md. Abdulla Al Mamun
- , Wei Cao
- & Jun-ichi Maruyama
-
Article
| Open AccessStructure and activation mechanism of the hexameric plasma membrane H+-ATPase
The plasma membrane H+ -ATPase is responsible for maintenance of the plasma membrane potential, which provides energy for the transport of nutrients, and the plasma membrane H+ -ATPase in S. cerevisiae (Pma1) is a P3A-type ATPase that assembles and functions as a hexamer. Here, the authors present the cryo-EM structures of autoinhibited and activated native Pma1 hexamers purified with endogenous lipids and they propose a mechanism for proton pumping across the membrane by this family of H+ -ATPases.
- Peng Zhao
- , Chaoran Zhao
- & Lin Bai
-
Article
| Open AccessInterplay of two transcription factors for recruitment of the chromatin remodeling complex modulates fungal nitrosative stress response
Plant and animal tissues produce nitric oxide and reactive nitrogen species that induce nitrosative stress in pathogens. Here, Jian et al. identify two transcriptional regulators in the phytopathogen Fusarium graminearum that control the nitrosative stress response by modulating the recruitment of a chromatin-remodelling complex at the promoters of the response genes.
- Yunqing Jian
- , Zunyong Liu
- & Zhonghua Ma
-
Article
| Open AccessFRQ-CK1 interaction determines the period of circadian rhythms in Neurospora
Circadian clocks control daily rhythms of molecular and physiological activities. Here, the authors show that the interaction between proteins FRQ and CK1, rather than FRQ stability, is a major rate-limiting step in circadian period determination in the model fungus Neurospora.
- Xiao Liu
- , Ahai Chen
- & Yi Liu
-
Article
| Open AccessA phosphorylated transcription factor regulates sterol biosynthesis in Fusarium graminearum
The fungus Fusarium graminearum is a pathogen of cereal crops. Here, Liu et al. identify a transcription factor that regulates sterol biosynthesis and virulence in this organism.
- Zunyong Liu
- , Yunqing Jian
- & Yanni Yin
-
Article
| Open AccessThe Candida albicans transcription factor Cas5 couples stress responses, drug resistance and cell cycle regulation
Cas5 is a transcriptional regulator of responses to cell wall stress in the fungal pathogen Candida albicans. Here, Xie et al. show that Cas5 also modulates cell cycle dynamics and responses to antifungal drugs.
- Jinglin L. Xie
- , Longguang Qin
- & Leah E. Cowen
-
Article
| Open AccessCell populations can use aneuploidy to survive telomerase insufficiency
The loss of telomeres is a catastrophic event and eukaryotes have evolved multiple strategies to overcome this. Here the authors show that Saccharomyces cerevisiaecan generate aneuploid survivors that upregulate telomerase to overcome telomere loss.
- Caroline Millet
- , Darya Ausiannikava
- & Svetlana Makovets
-
Article
| Open AccessLong non-coding RNA-mediated transcriptional interference of a permease gene confers drug tolerance in fission yeast
The presence of long non-coding RNAs (lncRNAs) is pervasive across genomes, yet few lncRNAs have clearly established mechanisms of action. Here the authors demonstrate that the fission yeast lncRNA nc-tgp1 regulates expression of the drug tolerance gene tgp1+ via+transcriptional interference.
- Ryan Ard
- , Pin Tong
- & Robin C. Allshire
-
Article |
Single yeast cells vary in transcription activity not in delay time after a metabolic shift
Individual cells respond differently to environmental stressors. Here, Schwabe et al.expose yeast cells to sulphur stress and show that small variations in response time combined with a high transient variability in transcript number contribute to stochasticity in response to this stress.
- Anne Schwabe
- & Frank J. Bruggeman
-
Article
| Open AccessA global non-coding RNA system modulates fission yeast protein levels in response to stress
Non-coding RNAs are widely expressed, yet their functions remain poorly understood. Here, Leong et al. identify a set of antisense RNAs elevated during the yeast stress response that directly correlate with reduced protein levels, indicating a general regulatory effect of antisense expression.
- Hui Sun Leong
- , Keren Dawson
- & Crispin J. Miller
-
Article |
Photoautotrophic hydrogen production by eukaryotic microalgae under aerobic conditions
Eukaryotic algae and cyanobacteria can produce hydrogen in the presence of little or no oxygen. Here, the authors show that two microalgal strains are capable of producing hydrogen under aerobic conditions, and provide new insights into the natural evolution of oxygen-tolerant hydrogenase.
- Jae-Hoon Hwang
- , Hyun-Chul Kim
- & Byong-Hun Jeon
-
Article |
Characterization of salt-adapted secreted lignocellulolytic enzymes from the mangrove fungus Pestalotiopsis sp.
Fungi associated with the roots of mangroves are thought to turn-over significant quantities of organic matter including lignocellulose. Arfi et al. investigate the lignocellolytic potential of a fungal species isolated from mangrove roots and the adaptation of its enzymatic repertoire to a high salinity environment.
- Yonathan Arfi
- , Didier Chevret
- & Eric Record
-
Article |
Organellar mechanosensitive channels in fission yeast regulate the hypo-osmotic shock response
Mechanosensitive channels are required to sense cell swelling in response to osmotic shock. Nakayamaet al.report that Msy1 and Msy2 are the fission yeast homologues of the bacterial mechanosensitive channel MscS, and are required for regulating intracellular calcium in response to cell swelling.
- Yoshitaka Nakayama
- , Kenjiro Yoshimura
- & Hidetoshi Iida