Featured
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| Open AccessProtein kinase A controls the hexosamine pathway by tuning the feedback inhibition of GFAT-1
The glutamine fructose-6-phosphate amidotransferase 1 (GFAT-1) is the rate-limiting enzyme in the hexosamine pathway producing uridine 5’-diphospho-N-acetyl-D-glucosamine (UDP-GlcNAc), an essential glycosylation precursor. Here, the authors dissect the mechanisms of GFAT-1 regulation by protein kinase A (PKA)-mediated phosphorylation.
- Sabine Ruegenberg
- , Felix A. M. C. Mayr
- & Martin S. Denzel
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Article
| Open AccessMutagenesis screen uncovers lifespan extension through integrated stress response inhibition without reduced mRNA translation
Aging is associated with declining protein homeostasis. Here, using a chemical mutagenesis screen for lifespan extension in C. elegans, the authors report that inhibition of the integrated stress response enhances longevity and protein homeostasis in a manner dependent on kin-35, without reducing protein synthesis.
- Maxime J. Derisbourg
- , Laura E. Wester
- & Martin S. Denzel
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Article
| Open AccessCortical structural differences in major depressive disorder correlate with cell type-specific transcriptional signatures
The correlation between brain structural changes in major depressive disorder (MDD) and gene expression is unclear. Here, the authors explore the correlation between cell type-specific gene expression changes and cortical structural difference in individuals with major depressive disorder.
- Jiao Li
- , Jakob Seidlitz
- & Wei Liao
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Article
| Open AccessIntegration of Alzheimer’s disease genetics and myeloid genomics identifies disease risk regulatory elements and genes
This study integrates Alzheimer’s disease (AD) GWAS data with myeloid cell genomics, and reports that myeloid active enhancers are most burdened by AD risk alleles. The authors also nominate candidate causal regulatory elements, variants and genes that likely modulate the risk for AD.
- Gloriia Novikova
- , Manav Kapoor
- & Alison M. Goate
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Article
| Open AccessGlobal discovery of lupus genetic risk variant allelic enhancer activity
Thousands of genetic variants have been associated with lupus, but causal variants and mechanisms are unknown. Here, the authors combine a massively parallel reporter assay with genome-wide ChIP experiments to identify risk variants with allelic enhancer activity mediated through transcription factor binding.
- Xiaoming Lu
- , Xiaoting Chen
- & Leah C. Kottyan
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Article
| Open AccessProtein context shapes the specificity of SH3 domain-mediated interactions in vivo
The SRC Homology 3 (SH3) domains mediate protein–protein interactions (PPIs). Here, the authors assess the SH3-mediated PPIs in yeast, and show that the identity of the protein itself and the position of the SH3 both affect the interaction specificity and thus the PPI-dependent cellular functions.
- Ugo Dionne
- , Émilie Bourgault
- & Christian R. Landry
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Article
| Open AccessChromatin information content landscapes inform transcription factor and DNA interactions
Interaction between transcription factors and chromatin is fundamental for genome organization and regulation. Here, the authors use information theory to measure signatures of organized chromatin resulting from transcription factor-chromatin interactions, termed chromatin information enrichment, and find that variations in the information encoded in chromatin architecture reflects functional biological variation.
- Ricardo D’Oliveira Albanus
- , Yasuhiro Kyono
- & Stephen C. J. Parker
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Article
| Open AccessA molecular quantitative trait locus map for osteoarthritis
Understanding the molecular effects of disease variants in relevant tissues is essential to understanding and treating disease. Here, the authors discover expression and protein quantitative trait loci in cartilage and synovium from 115 osteoarthritis patients to pinpoint genes of action and potential drug treatments.
- Julia Steinberg
- , Lorraine Southam
- & Eleftheria Zeggini
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Article
| Open AccessDeterminants of genome-wide distribution and evolution of uORFs in eukaryotes
Upstream open reading frames (uORFs) have widespread regulatory functions in eukaryotes, their genomic distribution and evolution is understudied. Here, the authors characterise ~17 million putative uORFs across 478 eukaryotic species, showing how evolution has shaped uORF contents and distribution.
- Hong Zhang
- , Yirong Wang
- & Jian Lu
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Article
| Open AccessLETR1 is a lymphatic endothelial-specific lncRNA governing cell proliferation and migration through KLF4 and SEMA3C
Long noncoding RNAs regulate tissue-specific gene expression. Here the authors profile lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs) and show a role of LEC-specific lncRNA, LETR1, in cell proliferation and migration.
- Luca Ducoli
- , Saumya Agrawal
- & Michael Detmar
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Article
| Open AccessImpaired eIF5A function causes a Mendelian disorder that is partially rescued in model systems by spermidine
eIF5A is critical for protein synthesis but has not yet been associated with congenital human disease. Here, the authors show that EIF5A variants cause a Mendelian disorder via reduced eIF5A-ribosome interactions and this phenotype is partially corrected by spermidine supplementation in yeast and zebrafish.
- Víctor Faundes
- , Martin D. Jennings
- & Siddharth Banka
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Article
| Open AccessA fast and efficient colocalization algorithm for identifying shared genetic risk factors across multiple traits
Statistical colocalisation is a method to identify causal genes and shared genetic aetiology across traits. Here, the authors describe HyPrColoc, an efficient Bayesian divisive clustering algorithm which integrates summary statistics from genome-wide association studies to detect clusters of colocalised traits from large numbers of traits.
- Christopher N. Foley
- , James R. Staley
- & Joanna M. M. Howson
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Article
| Open AccessConserved regulatory logic at accessible and inaccessible chromatin during the acute inflammatory response in mammals
Genetic elements that control inflammatory gene expression are not fully elucidated. Here the authors conduct a multi-species analysis of chromatin landscape and NF-κB binding in response to the proinflammatory cytokine TNFα, finding that conserved NF-κB bound regions are linked to enhancer activity and disease.
- Azad Alizada
- , Nadiya Khyzha
- & Michael D. Wilson
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Article
| Open AccessWhite pupae phenotype of tephritids is caused by parallel mutations of a MFS transporter
The white pupae (wp) phenotype has been used for decades to selectively remove females of tephritid species in genetic sexing, but the determining gene is unknown. Here, the authors show that wp phenotype is produced by parallel mutations in a Major Facilitator Superfamily domain containing gene across multiple species.
- Christopher M. Ward
- , Roswitha A. Aumann
- & Marc F. Schetelig
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Article
| Open AccessTwo novel venom proteins underlie divergent parasitic strategies between a generalist and a specialist parasite
Parasitism is a widespread evolutionary strategy. A study that spans functional and evolutionary genomics identifies the molecular basis and history underlying two genes that have mediated divergent parasitic strategies (specialist vs generalist) between two sister species of parasitoid wasp.
- Jianhua Huang
- , Jiani Chen
- & Shuai Zhan
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| Open AccessFunctional studies of GWAS variants are gaining momentum
Rapidly advancing genomic technologies and cross-disciplinary partnerships are accelerating the biological and clinical interpretation of genome-wide association studies, with some therapies developed based on these findings already being tested in clinical trials. The next decade promises further progress in understanding the function of genetic variants.
- Florence Lichou
- & Gosia Trynka
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Article
| Open AccessImproving the informativeness of Mendelian disease-derived pathogenicity scores for common disease
Pathogenicity scores are instrumental in prioritizing variants for Mendelian disease, yet their application to common disease is largely unexplored. Here, the authors assess the utility of pathogenicity scores for 41 complex traits and develop a framework to improve their informativeness for common disease.
- Samuel S. Kim
- , Kushal K. Dey
- & Alkes L. Price
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Article
| Open AccessConstruction of a complete set of Neisseria meningitidis mutants and its use for the phenotypic profiling of this human pathogen
The bacterium Neisseria meningitidis causes life-threatening meningitis and sepsis. Here, Muir et al. construct a complete collection of defined mutants in protein-coding genes of this organism, which they use to identify its essential genome and to shed light on the functions of multiple genes.
- Alastair Muir
- , Ishwori Gurung
- & Vladimir Pelicic
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Article
| Open AccessNuclear gene proximity and protein interactions shape transcript covariations in mammalian single cells
Gene expression covariation can be studied by single-cell RNA sequencing. Here the authors analyze intrinsically covarying gene pairs by eliminating the confounding effects in single-cell experiments and observe covariation of proximal genes and miRNA-induced covariation of target mRNAs.
- Marcel Tarbier
- , Sebastian D. Mackowiak
- & Marc R. Friedländer
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Article
| Open AccessThe structural variation landscape in 492 Atlantic salmon genomes
This study presents and validates a novel approach to reliably identify structural variations (SVs) in non-model genomes using whole genome sequencing, which was used to detect 15,483 SVs in 492 Atlantic salmon, shedding light on their roles in genome evolution and the genetic architecture of domestication.
- Alicia C. Bertolotti
- , Ryan M. Layer
- & Daniel J. Macqueen
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Article
| Open AccessEvaluating the informativeness of deep learning annotations for human complex diseases
Deep learning models have shown great promise in predicting regulatory effects from DNA sequence. Here the authors evaluate sequence-based epigenomic deep learning models and conclude that these models are not yet ready to inform our knowledge of human disease.
- Kushal K. Dey
- , Bryce van de Geijn
- & Alkes L. Price
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Article
| Open AccessHaploinsufficiency of RREB1 causes a Noonan-like RASopathy via epigenetic reprogramming of RAS-MAPK pathway genes
Mutations in RAS-MAPK pathway genes are implicated in Noonan-spectrum, yet up to 20% of cases have unknown cause. Here, the authors identify RREB1 underlying a 6p microdeletion RASopathy-like syndrome and show that RREB1, SIN3A and KDM1A form a transcriptional repressive complex to control methylation of MAPK pathway genes.
- Oliver A. Kent
- , Manipa Saha
- & Robert Rottapel
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Article
| Open AccessAnalysis of chromatin organization and gene expression in T cells identifies functional genes for rheumatoid arthritis
Although genome-wide association studies have identified genetic variation contributing to disease risk, assigning causal genes is challenging. Here, the authors generate ATAC-seq, Hi-C, Capture Hi-C and RNA-seq data in stimulated CD4+ T cells to identify functional enhancers and demonstrate interactions of expression quantitative trait loci with target genes in rheumatoid arthritis.
- Jing Yang
- , Amanda McGovern
- & Stephen Eyre
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Article
| Open AccessThe genomic landscape of Mongolian hepatocellular carcinoma
Mongolia has the highest incidence of—and mortality from—hepatocellular carcinoma (HCC) in the world. Here, the authors examine the genomic and transcriptomic landscape of Mongolian HCC, uncover novel driver mutations, and suggest distinct disease etiologies.
- Julián Candia
- , Enkhjargal Bayarsaikhan
- & Xin Wei Wang
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Article
| Open AccessDefining the ATPome reveals cross-optimization of metabolic pathways
Energy metabolism and ATP levels are controlled by an interlocking network of pathways. Here, the authors apply a genome-wide CRISPR screen to define genes that increase or decrease ATP levels to define the “ATPome”, a map of pathways that contribute to cellular ATP regulation.
- Neal K. Bennett
- , Mai K. Nguyen
- & Ken Nakamura
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Article
| Open AccessMultiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action
Large-scale screens of chemical and genetic vulnerabilities in cancer are typically limited to simple readouts of cell viability. Here, the authors develop a method for profiling post-perturbation transcriptional responses across large pools of cancer cell lines, enabling deep characterization of shared and context-specific responses.
- James M. McFarland
- , Brenton R. Paolella
- & Aviad Tsherniak
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Article
| Open AccessCRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs
Off-target editing remains a concern for therapeutic applications of CRISPR-Cas9. Here the authors present CRISPR GUARD, which uses very short non-cleaving gRNAs to prevent editing at off-target sites.
- Matthew A. Coelho
- , Etienne De Braekeleer
- & Benjamin J. M. Taylor
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Article
| Open AccessTesting and controlling for horizontal pleiotropy with probabilistic Mendelian randomization in transcriptome-wide association studies
Transcriptome-wide association studies integrate GWAS and transcriptome data to examine the molecular mechanisms underlying disease etiology. Here the authors present PMR-Egger, a powerful TWAS method based on probabilistic Mendelian Randomization.
- Zhongshang Yuan
- , Huanhuan Zhu
- & Xiang Zhou
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Article
| Open AccessA rare variant of African ancestry activates 8q24 lncRNA hub by modulating cancer associated enhancer
Genetic variants on chromosome 8q24 are associated with prostate cancer risk in men of African ancestry. Here the authors show that one of these variants, rs72725854 alters the enhancer activity in its region, which upon androgen stimulation, activates multiple oncogenic lncRNAs and c-myc.
- Kaivalya Walavalkar
- , Bharath Saravanan
- & Dimple Notani
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Article
| Open AccessHigh-performance CRISPR-Cas12a genome editing for combinatorial genetic screening
Reliable, multiplexed gene editing to uncover synergies between targets remains challenging. Here, the authors engineer AsCas12a and the crRNA to improve double knockout for synthetic sick/lethal interaction genetic screening.
- Rodrigo A. Gier
- , Krista A. Budinich
- & Junwei Shi
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Article
| Open AccessMapping effector genes at lupus GWAS loci using promoter Capture-C in follicular helper T cells
T cells are a major cell type involved in systemic lupus erythematosus (SLE). Here, the authors use promoter capture-C and ATAC-seq in human follicular T helper cells to identify SLE genes distant from GWAS loci (via 3D interaction) and validate the function of key regulatory elements and genes in vitro.
- Chun Su
- , Matthew E. Johnson
- & Andrew D. Wells
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Article
| Open AccessGermline de novo mutation rates on exons versus introns in humans
Evidence that somatic mutation rates in introns exceed those in exons challenges the molecular evolution tenet that mutation rate and sequence function are independent. Here, authors analyze germline de novo mutations and reveal no evidence for mutation rate differences between exons and introns.
- Miguel Rodriguez-Galindo
- , Sònia Casillas
- & Antonio Barbadilla
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Article
| Open AccessNOTCH1 activation compensates BRCA1 deficiency and promotes triple-negative breast cancer formation
BRCA1 mutation carriers have higher chances of developing triple-negative breast cancer (TNBC). Here, the authors use the Sleeping Beauty mutagenesis system in Brca1 deficient mice and identify 169 putative driver genes, of which NOTCH1 accelerates TNBC formation through promoting epithelial-mesenchymal transition and cell cycle progression.
- Kai Miao
- , Josh Haipeng Lei
- & Chu-Xia Deng
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Article
| Open AccessRSPO3 impacts body fat distribution and regulates adipose cell biology in vitro
Genetic variants at the RSPO3 locus are associated with waist-to-hip ratio (adjusted for BMI). Here, Loh et al. describe two independent RSPO3 signals that associate with body fat distribution, perform fine-mapping and explore the function of RSPO3 in human adipocyte biology and body fat distribution in zebrafish
- Nellie Y. Loh
- , James E. N. Minchin
- & Constantinos Christodoulides
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Article
| Open AccessA genome-wide gain-of-function screen identifies CDKN2C as a HBV host factor
Here the authors perform a gain-of-function screen and identify CDKN2C as a host factor for HBV replication, inducing cell cycle arrest and expression of HBV transcription enhancers. CDKN2C expression correlates with disease progression suggesting a potential role in HBV-induced liver disease.
- Carla Eller
- , Laura Heydmann
- & Thomas F. Baumert
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Article
| Open AccessGenomic adaptations to aquatic and aerial life in mayflies and the origin of insect wings
Genomic studies of paleopteran insects, such as mayflies, are needed to reconstruct early insect evolution. Here, Almudi and colleagues present the genome of the mayfly Cloeon dipterum and use transcriptomics to characterize its adaptations to distinct habitats and the origin of insect wings.
- Isabel Almudi
- , Joel Vizueta
- & Fernando Casares
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Article
| Open AccessEvolutionary conserved NSL complex/BRD4 axis controls transcription activation via histone acetylation
The MOF acetyltransferase-containing Non-Specific Lethal (NSL) complex is a broad transcription regulator and haploinsufficiency of its KANSL1 subunit results in the Koolen-de Vries syndrome in humans. Here, the authors identify the BET protein BRD4 as evolutionary conserved co-factor of the NSL complex and provide evidence that NSL-deposited histone acetylation induces BRD4 recruitment for transcription of constitutively active genes.
- Aline Gaub
- , Bilal N. Sheikh
- & Asifa Akhtar
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Article
| Open AccessFAM13A affects body fat distribution and adipocyte function
Genetic variants in the FAM13A locus have been associated with anthropometric and glycemic traits. Here, using fine-mapping, in vitro knockdown studies in pre-adipocytes and in vivo knockout in mice, the authors show that FAM13A is involved in regulating fat distribution and metabolic traits.
- Mohsen Fathzadeh
- , Jiehan Li
- & Joshua W. Knowles
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Article
| Open AccessAbundance of conserved CRISPR-Cas9 target sites within the highly polymorphic genomes of Anopheles and Aedes mosquitoes
Genetic variation in natural populations could represent gene drive resistant alleles, preventing successful application for population management. Here the authors survey 1280 genomes from three mosquito species and concludes natural variation will not be detrimental to deploying gene drive technology.
- Hanno Schmidt
- , Travis C. Collier
- & Gregory C. Lanzaro
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Article
| Open AccessNucleosome positioning stability is a modulator of germline mutation rate variation across the human genome
Nucleosome organization has been suggested to affect local mutation rates in the genome. Here, the authors analyse data on >300,000 human de novo mutations and high-resolution nucleosome maps and provide evidence that nucleosome positioning stability modulates germline mutation rate variation across the human genome.
- Cai Li
- & Nicholas M. Luscombe
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Article
| Open AccessPrioritizing disease and trait causal variants at the TNFAIP3 locus using functional and genomic features
While genome-wide association studies have yielded thousands of trait-associated loci, identifying causal variants remains challenging. Here, the authors perform seven genomics assays in various cell types to prioritize genetic variants in the TNFAIP3 locus, and report high-priority variants within disease-associated haplotypes.
- John P. Ray
- , Carl G. de Boer
- & Nir Hacohen
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Article
| Open AccessGene clustering and copy number variation in alkaloid metabolic pathways of opium poppy
The opium poppy has been a source of painkilling drugs synthesized by the benzylisoquinoline alkaloid pathway. Here, the authors report an improved genome assembly and reveal gene clustering and copy number variation in alkaloid metabolic pathways.
- Qiushi Li
- , Sukanya Ramasamy
- & Sam Yeaman
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Article
| Open AccessGenome-wide association and multi-omic analyses reveal ACTN2 as a gene linked to heart failure
Heart failure has a heterogeneous etiology and the genetic underpinnings are not well understood. Here, Arvanitis et al. perform GWAS meta-analysis including 10,976 heart failure cases and 437,573 controls, identify new loci near ABO and ACTN2 and show that deletion of a ACTN2 enhancer leads to reduced ACTN2 expression in differentiating cardiomyocytes.
- Marios Arvanitis
- , Emmanouil Tampakakis
- & Alexis Battle
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Article
| Open AccessIn situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila
In Drosophila, transcription is thought to be required for TAD formation, while the role of architectural proteins remains controversial. Here, the authors find that deletion of domain boundaries at the fly Notch locus results in TAD fusion and long-range topological defects, loss of architectural protein and RNA Pol II chromatin binding, and transcription defects.
- Rodrigo G. Arzate-Mejía
- , Angel Josué Cerecedo-Castillo
- & Félix Recillas-Targa
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Article
| Open AccessSingle-cell RNA-sequencing of differentiating iPS cells reveals dynamic genetic effects on gene expression
Studying the genetic effects on early stages of human development is challenging due to a scarcity of biological material. Here, the authors utilise induced pluripotent stem cells from 125 donors to track gene expression changes and expression quantitative trait loci at single cell resolution during in vitro endoderm differentiation.
- Anna S. E. Cuomo
- , Daniel D. Seaton
- & Oliver Stegle
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Article
| Open AccessDe novo emergence of adaptive membrane proteins from thymine-rich genomic sequences
There is increasing evidence that protein-coding genes can emerge de novo from noncoding genomic regions. Vakirlis et al. propose that sequences encoding transmembrane polypeptides can emerge de novo in thymine-rich genomic regions and provide organisms with fitness benefits.
- Nikolaos Vakirlis
- , Omer Acar
- & Anne-Ruxandra Carvunis
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Article
| Open AccessGenetic screens in isogenic mammalian cell lines without single cell cloning
Isogenic pairs of cell lines are powerful tools but time-consuming to generate. Here the authors conduct genome-wide genetic interactions screens of ‘anchor’ genes with SaCas9 and SpCas9.
- Peter C. DeWeirdt
- , Annabel K. Sangree
- & John G. Doench
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Article
| Open AccessA cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion
Pre-malignant cells harbouring oncogenic mutations can populate and spread throughout a tissue. Here, using a rainbow mouse system, the authors explore how clonal expansion in the mouse intestine might explain high levels of intra-tumoural heterogeneity observed in the disease.
- Peter G. Boone
- , Lauren K. Rochelle
- & Joshua C. Snyder
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Article
| Open AccessFull-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus
It is challenging to characterize diverse transcript isoforms by short-read sequencing. Here the authors report full-length transcriptomes in rat hippocampus by hybrid-sequencing, predict isoform-specific translational status, and reconstruct open reading frames validated by mass spectrometry.
- Xi Wang
- , Xintian You
- & Wei Chen