Electron microscopy articles within Nature

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  • Article |

    Cryo-electron microscopy and tomography structures of reconstituted and endogenous human mRNA ribonucleoprotein complexes bound to the transcription–export complex reveal how mRNAs are packaged and recognized for nuclear export.

    • Belén Pacheco-Fiallos
    • , Matthias K. Vorländer
    •  & Clemens Plaschka

  • Article
    | Open Access

    Cryo-electron microscopy analysis of the Deinococcus radiodurans ISDra2 TnpB in complex with its cognate ωRNA and target DNA provides insights into the mechanism of TnpB function and the evolution of CRISPR–Cas12 effectors.

    • Ryoya Nakagawa
    • , Hisato Hirano
    •  & Osamu Nureki

  • Article
    | Open Access

    The reaction coordinate of aminoacyl-tRNA movement is altered on the human ribosome and the process is an order of magnitude slower compared with bacteria due to eukaryote-specific structural elements in the human ribosome and in the elongation factor eEF1A.

    • Mikael Holm
    • , S. Kundhavai Natchiar
    •  & Scott C. Blanchard

  • Article
    | Open Access

    The authors report the cryogenic electron microscopy structures of human GSDMB in complex with Shigella IpaH7.8 and the GSDMB pore, shedding light on the molecular mechanisms of Shigella IpaH7.8 recognition and targeting of GSDMs and GSDMB pore formation.

    • Chengliang Wang
    • , Sonia Shivcharan
    •  & Jianbin Ruan

  • Article |

    The structural basis for the molecular mechanism of recognition of different IgM forms by the IgM receptor FcμR is determined using crystallography and cryo-electron microscopy.

    • Yaxin Li
    • , Hao Shen
    •  & Junyu Xiao

  • Article
    | Open Access

    A structure–function analysis of cystic fibrosis transmembrane conductance regulator shows its two nucleotide-binding domains dimerize before channel opening, and reveals a mechanism through which conformational changes in the channel regulate chloride conductance.

    • Jesper Levring
    • , Daniel S. Terry
    •  & Jue Chen

  • Article |

    Through the use of cryo-electron microscopy and molecular dynamics stimulations, mechanistic insight into the binding of an odorant to the human odorant receptor OR51E2 is provided.

    • Christian B. Billesbølle
    • , Claire A. de March
    •  & Aashish Manglik

  • Article
    | Open Access

    Structural and biochemical studies of the Mycobacterium smegmatis hydrogenase Huc provides insights into how [NiFe] hydrogenases oxidize trace amounts of atmospheric hydrogen and transfer the electrons liberated via quinone transport.

    • Rhys Grinter
    • , Ashleigh Kropp
    •  & Chris Greening

  • Article |

    The active-state structure of human DICER bound to pre-miRNA reveals the structural basis for the specificity of DICER in how it selects substrates in a sequence dependent manner, and sheds light on DICER-related diseases.

    • Young-Yoon Lee
    • , Hansol Lee
    •  & Soung-Hun Roh

  • Article
    | Open Access

    The authors report the cryo-EM structure of human A3G bound to HIV-1 Vif, and the hijacked cellular proteins that promote ubiquitin-mediated proteolysis, suggesting how Vif antagonizes A3G by intercepting it to prevent viral restriction.

    • Yen-Li Li
    • , Caroline A. Langley
    •  & John D. Gross

  • Article |

    Cryo-EM is used to visualize the SARS-CoV-2 RTC bound to each of the natural NTPs as well as remdesivir triphosphate (RDV-TP) in states poised for incorporation, explaining the interactions required for NTP recognition and RDV-TP selectivity.

    • Brandon F. Malone
    • , Jason K. Perry
    •  & Seth A. Darst

  • Article |

    A cryo-electron microscopy analysis of the Chlamydomonas reinhardtii TOC–TIC supercomplex reveals that Tic214 traverses the chloroplast inner membrane, the intermembrane space and the outer membrane, connecting the TOC complex with the TIC proteins.

    • Hao Liu
    • , Anjie Li
    •  & Zhenfeng Liu

  • Article
    | Open Access

    Structural studies of the ribosome-associated endoplasmic reticulum translocon complex based on cryo-electron tomography and molecular modelling reveal multiple intermediate states and interactions between the components of the complex and its cofactors.

    • Max Gemmer
    • , Marten L. Chaillet
    •  & Friedrich Förster

  • Article
    | Open Access

    Cryogenic-electron microscopy is used to determine the structure of TFEB as presented to mTORC1 for phosphorylation and an explanation is found for the strong dependence of TFEB phosphorylation on FLCN and the RagC GDP state.

    • Zhicheng Cui
    • , Gennaro Napolitano
    •  & James H. Hurley

  • Article |

    Mass spectrometry and structural studies demonstrate the specific changes in protein composition that accompany the transition of ribosomes in zebrafish and Xenopus eggs from a dormant to an active state during early embryogenesis.

    • Friederike Leesch
    • , Laura Lorenzo-Orts
    •  & Andrea Pauli

  • Article
    | Open Access

    Cryo-electron microscopy structures of Staphylococcus aureus BlaR1 reveal dynamic signalling states regulating broad spectrum β-lactam antibiotic resistance through cleavage of the transcriptional repressor BlaI and induced expression of the β-lactamase blaZ and the β-lactam-resistant cell-wall transpeptidase mecA.

    • J. Andrew N. Alexander
    • , Liam J. Worrall
    •  & Natalie C. J. Strynadka

  • Article
    | Open Access

    Structural analysis of Cas12a2, a CRISPR-associated nuclease that nonspecifically cleaves ssRNA, ssDNA and dsDNA, reveals a complete activation pathway involved in the abortive infection system protecting cells against invasion.

    • Jack P. K. Bravo
    • , Thomson Hallmark
    •  & David W. Taylor

  • Article |

    Structures of the human METTL1–WDR4 complex are revealed, providing molecular insights into substrate recognition, modification and catalytic regulation by the N7-methylguanosine methyltransferase complex.

    • Jiazhi Li
    • , Longfei Wang
    •  & Richard I. Gregory

  • Article |

    RibosomeST—a ribosome with a specialized nascent polypeptide exit tunnel—cotranslationally regulates the folding of a subset of male germ-cell-specific proteins that are essential for the formation of sperm.

    • Huiling Li
    • , Yangao Huo
    •  & Jiahao Sha

  • Article |

    Mutations in the sodium/iodide symporter (NIS) cause congenital hypothyroidism, and our results yield insights into how NIS selects, couples and translocates anions, thereby establishing a framework for understanding NIS function.

    • Silvia Ravera
    • , Juan Pablo Nicola
    •  & Nancy Carrasco

  • Article |

    We report cryogenic electron microscopy structures of disc-shaped active NLRP3 oligomers in complex with NEK7 and ASC, and propose that the role of NEK7 is to transform NLRP3 into the active NLRP3 inflammasome disc.

    • Le Xiao
    • , Venkat Giri Magupalli
    •  & Hao Wu

  • Article
    | Open Access

    Structural studies of the CRISPR-associated transposon comprising Cas12k, TnsC, TnsB and TniQ from Scytonema hofmannii using cryo-electron microscopy reveal insights into the architecture and mechanism of RNA-guided DNA transposition.

    • Jung-Un Park
    • , Amy Wei-Lun Tsai
    •  & Elizabeth H. Kellogg

  • Article
    | Open Access

    Structural insights into the poly-ADP-ribosyltransferase tankyrase reveal its filamentous architecture and illustrate how assembly controls catalytic and non-catalytic functions.

    • Nisha Pillay
    • , Laura Mariotti
    •  & Sebastian Guettler

  • Article
    | Open Access

    The cryo-EM structure of the yeast SEA complex suggests that SEACAT functions as a scaffold for binding TORC1 regulators.

    • Lucas Tafur
    • , Kerstin Hinterndorfer
    •  & Robbie Loewith

  • Article |

    Electrophysiological, structural and biochemical studies on the bestrophin-2 anion channel reveal asymmetric permeability to glutamate and show that it forms a cooperative machinery in complex with glutamine synthetase for glutamate release.

    • Aaron P. Owji
    • , Kuai Yu
    •  & Tingting Yang

  • Article
    | Open Access

    Cryo-electron microscopy structures of skeletal F-actin show solvent-driven rearrangements governing actin filament assembly and aging with potential application in design of drugs and small molecules for imaging and therapy.

    • Wout Oosterheert
    • , Björn U. Klink
    •  & Stefan Raunser

  • Article |

    Two cyclic dinucleotide molecules bind within the cavity of human SLC19A1 as a compact dual-molecule unit, whereas folate and antifolate bind to SLC19A1 as a monomer and occupy a distinct pocket of the cavity.

    • Qixiang Zhang
    • , Xuyuan Zhang
    •  & Pu Gao

  • Article |

    Biochemical and structural analysis of intermediates during multipass membrane protein biogenesis showed how an intramembrane chaperone guides nascent membrane proteins to a semi-enclosed lipid-filled cavity where they are inserted and folded correctly.

    • Luka Smalinskaitė
    • , Min Kyung Kim
    •  & Ramanujan S. Hegde

  • Article |

    Cryo-electron microscopy structures of the mouse class M immunoglobulin B cell receptor complex provide insight into B cell receptor subunit assembly, domain architecture and signalling mechanism.

    • Ying Dong
    • , Xiong Pi
    •  & Hao Wu

  • Article
    | Open Access

    Structural studies of the native transmembrane channel-like protein 1 (TMC-1) mechanosensory transduction channel complex of Caenorhabditis elegans reveal the subunit composition and the roles of protein–membrane interactions in the conversion of mechanical force to ion channel activity.

    • Hanbin Jeong
    • , Sarah Clark
    •  & Eric Gouaux

  • Article
    | Open Access

    The cryogenic-electron microscopy structure of the D. thermocuniculi IsrB protein in complex with its cognate ωRNA and a target DNA shows that the RNA-dominant IsrB effector complex shares a common scaffold with the protein-dominant Cas9 effector complex.

    • Seiichi Hirano
    • , Kalli Kappel
    •  & Feng Zhang

  • Article |

    The cryo-electron microscopy structure of NuA4 from Saccharomyces cerevisiae bound to the nucleosome illustrates how NuA4 is assembled and provides mechanistic insights into nucleosome recognition and transcription co-activation by a histone acetyltransferase.

    • Keke Qu
    • , Kangjing Chen
    •  & Zhucheng Chen

  • Article
    | Open Access

    Cryo-electron tomography is used to reveal the structural dynamics and functional diversity of translating ribosomes in Mycoplasma pneumoniae, providing insight into the translation elongation cycle inside cells and how it is reshaped by antibiotics.

    • Liang Xue
    • , Swantje Lenz
    •  & Julia Mahamid

  • Article |

    Structural studies of the Ebola virus polymerase complex provide insights into its function and demonstrate the structural basis of its inhibition by suramin.

    • Bin Yuan
    • , Qi Peng
    •  & Yi Shi

  • Article
    | Open Access

    Evolutionary conservation of plant receptor structure allowed for generation of new variants of wheat and barley nucleotide-binding leucine-rich repeat receptors (NLRs) that recognize AvrSr35 of the wheat stem rust pathogen, supporting proof of principle for structure-based engineering of NLRs for crop improvement.

    • Alexander Förderer
    • , Ertong Li
    •  & Jijie Chai

  • Article |

    A structural analysis focusing on plant immunity reveals how LRR-containing receptor-like proteins recognize pathogenic ligands and consequently become activated, with the data suggesting that these proteins target pathogens through two different mechanisms.

    • Yue Sun
    • , Yan Wang
    •  & Jijie Chai

  • Article |

    Cryo-electron microscopy studies of Escherichia coli complex I suggest a conserved mechanism of coupled proton transfers and electrostatic interactions that result in proton ejection from the complex exclusively at the distal NuoL subunit.

    • Vladyslav Kravchuk
    • , Olga Petrova
    •  & Leonid Sazanov

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