Article
|
Open Access
Featured
-
-
Article |
RNA polymerase II associates with active genes during DNA replication
Protein complexes containing RNA polymerase II and immature RNA are associated with active genes immediately after replication, suggesting that transmission of active transcriptional states to daughter cells may not require any additional epigenetic bookmarks.
- Tyler K. Fenstermaker
- , Svetlana Petruk
- & Alexander Mazo
-
Article
| Open AccessReconstitution of a telomeric replicon organized by CST
The Polα–primase-associated CST complex organizes telomeric C-strand DNA synthesis, and, in combination with telomerase, it carries out complete replication of the single-stranded DNA overhang found at human telomeres.
- Arthur J. Zaug
- , Karen J. Goodrich
- & Thomas R. Cech
-
Article |
Structures of the human CST-Polα–primase complex bound to telomere templates
A structural analysis demonstrates how the single-stranded DNA-binding accessory protein complex CST physically organizes the human DNA polymerase-α–primase complex for efficient primer synthesis during telomere replication.
- Qixiang He
- , Xiuhua Lin
- & Ci Ji Lim
-
Article |
Structure of Tetrahymena telomerase-bound CST with polymerase α-primase
Cryo-electron microscopy structures of Tetrahymena thermophila telomerase-bound Ctc1–Stn1–Ten1 and DNA polymerase α–primase provide insights into the molecular mechanisms underlying telomere replication and maintenance.
- Yao He
- , He Song
- & Juli Feigon
-
Article |
Fast and efficient DNA replication with purified human proteins
A biochemical reconstitution of human replisomes that provides a system for future studies of DNA metabolic processes.
- Yasemin Baris
- , Martin R. G. Taylor
- & Joseph T. P. Yeeles
-
Article |
Molecular basis for the initiation of DNA primer synthesis
The molecular determinants for primer synthesis are identified within the catalytic domain of primase-polymerase enzymes, elucidating the mechanisms underlying initiation of primer synthesis.
- Arthur W. H. Li
- , Katerina Zabrady
- & Aidan J. Doherty
-
Article |
CDC7-independent G1/S transition revealed by targeted protein degradation
In addition to CDC7, the cell cycle kinase CDK1 has a pivotal role in the G1/S transition of cells, a finding that revises our current understanding of cell cycle progression.
- Jan M. Suski
- , Nalin Ratnayeke
- & Piotr Sicinski
-
Article |
Structures of telomerase at several steps of telomere repeat synthesis
Cryo-electron microscopy structures of Tetrahymena telomerase with telomeric DNA at several steps of nucleotide addition provide insights into the structural basis of telomere repeat synthesis.
- Yao He
- , Yaqiang Wang
- & Juli Feigon
-
Article |
Tracking break-induced replication shows that it stalls at roadblocks
A method of tracking break-induced replication reveals the details of this repair process and shows that it can be impaired by certain genomic elements and by transcription.
- Liping Liu
- , Zhenxin Yan
- & Anna Malkova
-
Article |
Equilibrium between nascent and parental MCM proteins protects replicating genomes
Mother cells recycle parental MCMs and simultaneously synthesize nascent MCMs, both of which are inherited by daughter cells, in which the former are preferentially used to form active replisomes and the latter adjust the pace of replisome movement to minimize errors during DNA replication.
- Hana Sedlackova
- , Maj-Britt Rask
- & Jiri Lukas
-
Article |
Fatty acid carbon is essential for dNTP synthesis in endothelial cells
This study identifies a crucial role for fatty acid oxidation (FAO) in endothelial cells during angiogenesis, and reveals that fatty-acid-derived carbons are used for the de novo synthesis of nucleotides, and hence FAO stimulates vessel sprouting by increasing endothelial cell proliferation.
- Sandra Schoors
- , Ulrike Bruning
- & Peter Carmeliet
-
Article |
Regulated eukaryotic DNA replication origin firing with purified proteins
It has long been a goal to reconstitute eukaryotic DNA replication; here a purified in vitro system from budding yeast containing 16 factors, themselves composed of 42 polypeptides, fulfils the staged process of origin-dependent initiation, including its regulation by kinases.
- Joseph T. P. Yeeles
- , Tom D. Deegan
- & John F. X. Diffley
-
Article |
Lagging-strand replication shapes the mutational landscape of the genome
The emRiboSeq sequencing method is used to track polymerase activity genome-wide in vivo; despite Okazaki fragment processing, DNA synthesized by error-prone polymerase-α (Pol-α) is retained in vivo and comprises ∼1.5% of the genome, establishing Pol-α as an important source of genomic variability and providing a mechanism for site-specific variation in nucleotide substitution rates.
- Martin A. M. Reijns
- , Harriet Kemp
- & Martin S. Taylor
-
Letter |
Pif1 helicase and Polδ promote recombination-coupled DNA synthesis via bubble migration
This paper demonstrates that Pif1 helicase works with polymerase d to promote DNA synthesis through a migrating D-loop, a mechanism used to copy tens of kilobases during repair of chromosome breaks by break-induced replication (BIR).
- Marenda A. Wilson
- , YoungHo Kwon
- & Grzegorz Ira
-
Letter |
Avoiding chromosome pathology when replication forks collide
The site of collision between two chromosome replication forks can be used to reinitiate replication independent of an active origin, with potentially pathogenic effects.
- Christian J. Rudolph
- , Amy L. Upton
- & Robert G. Lloyd
-
Article |
Intrinsic coupling of lagging-strand synthesis to chromatin assembly
Genome-wide deep sequencing of Okazaki fragments in S. cerevisae reveals a connection between lagging-strand synthesis and chromatin assembly.
- Duncan J. Smith
- & Iestyn Whitehouse