DNA probes articles within Nature Communications

Featured

• Article
  23 March 2020 | Open Access

  Size-selective molecular recognition based on a confined DNA molecular sieve using cavity-tunable framework nucleic acids

  Size-selective discrimination is an issue in biosensing. Here, the authors report on a size selective DNA nanocage which excludes agents based on size and protects the probes against degradation, and demonstrate the discrimination between mature and precursor miRNA.

  • Xiaoyi Fu
  • , Guoliang Ke
  •  & Weihong Tan

• Article
  13 February 2018 | Open Access

  Detecting RNA base methylations in single cells by in situ hybridization

  Methylated RNA bases influence many life processes, but current detection methods lack the ability to detect individual methylations in single cells. Here, the authors use fluorescence hybridization probes sensitive to methylation to detect specific epitranscriptomic modifications at the single-cell level.

  • Rohan T. Ranasinghe
  • , Martin R. Challand
  •  & David Klenerman

• Article
  18 June 2015 | Open Access

  The impact of DNA intercalators on DNA and DNA-processing enzymes elucidated through force-dependent binding kinetics

  DNA intercalators, a type of fluorescent probes widely used to visualize DNA, can perturb DNA structure and stability. Here, the authors show how DNA-binding affinity can be tuned using DNA tension, ionic strength and dye species, and how this can be used to minimize DNA structural perturbations.

  • Andreas S. Biebricher
  • , Iddo Heller
  •  & Gijs J. L. Wuite