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| Open AccessMyeloid cells interact with a subset of thyrocytes to promote their migration and follicle formation through NF-κB
The mechanism underlying thyroid follicle formation is not well understood. Here, the authors show that a subgroup of NF-κB-activated thyrocytes, through interactions with myeloid cells, exhibit increased migration capacity to generate new follicles.
- Yang Rui-Meng
- , Song Shi-Yang
- & Song Huai-Dong
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Article
| Open AccessTwo Rac1 pools integrate the direction and coordination of collective cell migration
Previous studies suggested a chemokine receptor governed gradient of Rac1 activity is essential for collective guidance of Drosophila border cells. Here, Zhou et al. report that two distinct Rac1 pools at protrusions and cables, not Rac1 activity gradient, integrate the direction and coordination for collective guidance.
- Sijia Zhou
- , Peng Li
- & Xiaobo Wang
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Article
| Open AccessPTEN inhibits AMPK to control collective migration
Pten is a tumour suppressor gene that is associated with highly invasive cancers such as glioblastoma. Here the authors show that PTEN loss results in increased migratory behaviour, which can be countered by targeting AMPK activity.
- Florent Peglion
- , Lavinia Capuana
- & Sandrine Etienne-Manneville
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| Open AccessTopography-induced large-scale antiparallel collective migration in vascular endothelium
The physical environment dictates the emergence of specific patterns of collective cell migration. Here, authors show that unconfined endothelial monolayers on microgroove substrates exhibit an original pattern of antiparallel cell streams.
- Claire Leclech
- , David Gonzalez-Rodriguez
- & Abdul I. Barakat
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Article
| Open AccessPulmonary fibrosis distal airway epithelia are dynamically and structurally dysfunctional
Environmental and genetic risk factors affect the distal airway epithelium in idiopatic pulmonary fibrosis (IPF) but the role of the epithelium in IPF remains unclear. Here the authors show that pathologic activation of the ERBB-YAP axis induces dynamic and structural dysfunction in the distal airway epithelium eliciting a pro-fibrotic phenotype in mesenchymal cells.
- Ian T. Stancil
- , Jacob E. Michalski
- & David A. Schwartz
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Article
| Open AccessA junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin trafficking for endothelial migration and angiogenesis
Communication between endothelial leader and follower cells during collective cell migration is crucial for vascular development. Here, the authors show that PACSIN2 guides collective cell migration and angiogenesis by recruiting a protein trafficking complex to asymmetric cell-cell junctions, controlling local junction plasticity.
- Tsveta S. Malinova
- , Ana Angulo-Urarte
- & Stephan Huveneers
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Article
| Open AccessAn ARF GTPase module promoting invasion and metastasis through regulating phosphoinositide metabolism
The signalling pathways underpinning cell growth and invasion use overlapping components, yet how mutually exclusive responses occur is unclear. Here, the authors show that alternate isoforms of the ARF GTPase exchange factor IQSEC1 direct phosphoinositide metabolism to control this switch.
- Marisa Nacke
- , Emma Sandilands
- & David M. Bryant
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Article
| Open AccessIn primary airway epithelial cells, the unjamming transition is distinct from the epithelial-to-mesenchymal transition
During repair, development, or cancer metastasis, epithelial cells can become migratory through partial or full epithelial to mesenchymal transition (EMT). Here, the authors report that differentiated epithelial collectives may undergo cooperative and collective migration without evidence of partial EMT through an unjamming transition (UJT).
- Jennifer A. Mitchel
- , Amit Das
- & Jin-Ah Park
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Article
| Open AccessEpithelial invagination by a vertical telescoping cell movement in mammalian salivary glands and teeth
Mechanisms that regulate epithelial bending mainly link to cell shape changes, for example, the formation of wedge shaped cells. Here, the authors identify a different cell behaviour in the salivary glands and teeth where initial invagination arises by a coordinated vertical cell movement.
- Jingjing Li
- , Andrew D. Economou
- & Jeremy B. A. Green
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Article
| Open AccessSubpopulation targeting of pyruvate dehydrogenase and GLUT1 decouples metabolic heterogeneity during collective cancer cell invasion
The presence of phenotypic heterogeneity in collectively invading cells suggests cooperation amongst distinct subtypes of cells to promote invasion and metastasis. Here, the authors use chemical biology tools and report metabolic heterogeneity within the lung cancer collective invasion pack.
- R. Commander
- , C. Wei
- & A. I. Marcus
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Article
| Open AccessMisshapen coordinates protrusion restriction and actomyosin contractility during collective cell migration
Directed motility of cell clusters requires coordination of protrusion formation at the front of leader cells and contractility at the rear. Here the authors show that the kinase Misshapen restricts protrusions to the leader cell and promotes contractile forces at the rear of the cluster.
- Cédric Plutoni
- , Sarah Keil
- & Gregory Emery
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Article
| Open AccessFlagellar cAMP signaling controls trypanosome progression through host tissues
Trypanosoma brucei probably relies on chemotactic signals for movement through tsetse fly tissues, but the molecular basis is unknown. Here, the authors show that flagellar cAMP signaling is required for traversal of the peritrophic matrix and that, without it, parasites are trapped in the midgut lumen.
- Sebastian Shaw
- , Stephanie F. DeMarco
- & Kent L. Hill
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| Open AccessDiscoidin-domain receptor coordinates cell-matrix adhesion and collective polarity in migratory cardiopharyngeal progenitors
In Ciona, cardiopharyngeal progenitors migrate as polarized pairs of cells between the epidermis and the endoderm. Here the authors show that the endoderm secretes a particular collagen, which promotes Discoidin domain receptor activity to control cell-matrix adhesion and collective polarity.
- Yelena Y. Bernadskaya
- , Saahil Brahmbhatt
- & Lionel Christiaen
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Article
| Open AccessCalcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks
The molecular mechanisms regulating mesenchymal cell movements are unclear. Here, the authors show in chicken feather elongation that SHH/WNT signalling establishes gap-junctions, enabling synchronized Ca2 + oscillations to emerge for and in turn coordinating directed cell migration.
- Ang Li
- , Jung-Hwa Cho
- & Cheng-Ming Chuong
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| Open AccessBlood flow-induced Notch activation and endothelial migration enable vascular remodeling in zebrafish embryos
The mechanisms of sensing of blood flow and responses to it in vascular remodelling are poorly understood. Here, the authors show that in zebrafish blood flow controls both the fate determination of individual blood vessels and the arterial versus venous identity of endothelial cells lining them.
- Bart Weijts
- , Edgar Gutierrez
- & Eugene Tkachenko
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Article
| Open AccessCoordinated collective migration and asymmetric cell division in confluent human keratinocytes without wounding
Epithelial sheet migration requires polarized and coordinated cell movement. Here, the authors demonstrate serum-activated collective migration followed by polarized asymmetric cell divisions in otherwise quiescent human keratinocyte monolayers in the absence of wound edges.
- Emma Lång
- , Anna Połeć
- & Stig Ove Bøe
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Article
| Open AccessTBC1d24-ephrinB2 interaction regulates contact inhibition of locomotion in neural crest cell migration
Group migration of cranial neural crest cells depends upon Eph-ephrin signalling. Here, the authors show that TBC1d24 complexes with ephrinB2 via Dishevelled to induce contact inhibition of locomotion by reducing membrane E-cadherin. Eph receptor binding dissolves this complex and disrupts migration.
- Jaeho Yoon
- , Yoo-Seok Hwang
- & Ira O. Daar
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| Open AccessMechanical interactions among followers determine the emergence of leaders in migrating epithelial cell collectives
During collective cell migration, how leader cells emerge is poorly understood. Here, the authors find that small groups of mechanically-interacting follower cells pull on the future leaders to stochastically elect them to their fate.
- Medhavi Vishwakarma
- , Jacopo Di Russo
- & Joachim P. Spatz
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Article
| Open AccessSpatial self-organization resolves conflicts between individuality and collective migration
How bacteria migrate collectively despite individual phenotypic variation is not understood. Here, the authors show that cells spontaneously sort themselves within moving bands such that variations in individual tumble bias, a determinant of gradient climbing speed, are compensated by the local gradient steepness experienced by individuals.
- X. Fu
- , S. Kato
- & T. Emonet
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| Open AccessEmergent patterns of collective cell migration under tubular confinement
Collective epithelial behaviours are studied in vitro in the context of flat sheets but a system to mimic tubular systems is lacking. Here, the authors develop a method to study collective behaviour in lumenal structures and show that several features depend on the extent of tubular confinement and/or curvature.
- Wang Xi
- , Surabhi Sonam
- & Chwee Teck Lim
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| Open AccessTyrosine dephosphorylated cortactin downregulates contractility at the epithelial zonula adherens through SRGAP1
Epithelial cell-cell adhesions are contractile junctions, but whether contractility can be down-regulated is not known. Here the authors report how tyrosine dephosphorylation of the cytoskeletal scaffold, cortactin, recruits the RhoA antagonist SRGAP1 to relax adherens junctions in response to HGF.
- Xuan Liang
- , Srikanth Budnar
- & Alpha S. Yap
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| Open AccessImage-guided genomics of phenotypically heterogeneous populations reveals vascular signalling during symbiotic collective cancer invasion
The mechanisms linking phenotypic heterogeneity to collective cancer invasion are unclear. Here the authors develop an image-guided genomic technique to select and amplify leader and follower cells fromin vitroinvading cell packs and find a cooperative symbiotic relationship between these two cell populations.
- J. Konen
- , E. Summerbell
- & A. I. Marcus
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| Open AccessQuantitative 3D analysis of complex single border cell behaviors in coordinated collective cell migration
Quantifying cell behavioursin vivo is essential to understanding the mechanisms of collective cell migration. Here the authors present an image analysis toolkit, CCMToolKit, to describe and characterize various modes of coordinated cell movements accompanying collective cell migration in Drosophilaborder cells.
- Adam Cliffe
- , David P. Doupé
- & Weimiao Yu
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Notch1–Dll4 signalling and mechanical force regulate leader cell formation during collective cell migration
Many forms of collective cell migration are directed by specialized leader cells that have a distinct protrusive phenotype. Riahi et al.show that lateral inhibition mediated by Notch1–Dll4 signalling determines the stochastic emergence of leader cells in epithelial monolayers.
- Reza Riahi
- , Jian Sun
- & Pak Kin Wong