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| Open AccessIFT cargo and motors associate sequentially with IFT trains to enter cilia of C. elegans
Intraflagellar transport is essential for the formation and function of cilia. Here, the authors use single-molecule imaging in live C. elegans to show that transport trains are formed by the sequential attachment of proteins before departing into the cilium.
- Aniruddha Mitra
- , Elizaveta Loseva
- & Erwin J. G. Peterman
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Article
| Open AccessUncovering structural themes across cilia microtubule inner proteins with implications for human cilia function
The inside surface of microtubules contains so-called microtubule inner proteins, but little is known about their identity. Here the authors use bioinformatics to identify structural motifs within this class of proteins and potential new members.
- Jens S. Andersen
- , Aaran Vijayakumaran
- & Kenneth Bødtker Schou
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| Open AccessMolecular basis promoting centriole triplet microtubule assembly
Centrioles are characterized by an atypical triplet microtubule structure. Here, the authors discover that the ciliopathy protein HYLS1 promotes the assembly of triplet microtubules within human centrioles.
- Yutaka Takeda
- , Takumi Chinen
- & Daiju Kitagawa
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Article
| Open AccessMulti-scale structures of the mammalian radial spoke and divergence of axonemal complexes in ependymal cilia
Radial spokes (RS) are crucial in coordinating ciliary motility. Here, authors use cryo-EM and cryo-ET to gain insight into mammalian RS divergence in ependymal cilia, RS assembly mechanism and the structure-function relationships of ciliary and flagellar axonemes.
- Xueming Meng
- , Cong Xu
- & Yao Cong
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Article
| Open AccessThe AMPK-Sirtuin 1-YAP axis is regulated by fluid flow intensity and controls autophagy flux in kidney epithelial cells
Urinary flow is sensed by renal cells but its intensity is dysregulated in renal diseases. Here, the authors report that physiological flow inhibits YAP to promote autophagy, while pathological flow leads to YAP activation and autophagy inhibition.
- Aurore Claude-Taupin
- , Pierre Isnard
- & Nicolas Dupont
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Article
| Open AccessCXCL12 targets the primary cilium cAMP/cGMP ratio to regulate cell polarity during migration
Regulation of cell polarity is key to ensure directed cell migration. Here, Atkins et al. identify the primary cilium cAMP/cGMP ratio as a master regulator of the cell polarity of migrating cortical interneurons downstream of the CXCL12 chemokine.
- Melody Atkins
- , Maud Wurmser
- & Christine Métin
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Article
| Open AccessFAP106 is an interaction hub for assembling microtubule inner proteins at the cilium inner junction
Microtubule inner proteins (MIPs) contribute to species-specific motility characteristics but are largely unstudied. Here, the authors combine functional, structural and proteomic analysis in T. brucei to advance fundamental understanding of MIP assembly and identify trypanosome-specific MIPs required for motility.
- Michelle M. Shimogawa
- , Angeline S. Wijono
- & Kent L. Hill
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| Open AccessNative doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins
Using cryo-EM, the authors identified 42 MIPs, including outer junction protein CFAP77 and outer dense fibers, in native doublet microtubules of Tetrahymena thermophila. Knockout of CFAP77 reduced ciliary beat frequency and led to outer junction damage.
- Shintaroh Kubo
- , Corbin S. Black
- & Khanh Huy Bui
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Article
| Open AccessA stress-induced cilium-to-PML-NB route drives senescence initiation
Exposure to irreparable stresses induces transient ciliogenesis, enabling communication with PML-NBs via a FBF1 pathway to trigger senescence in mammalian cells. Fbf1 ablation reduces senescence and associated health decline in mice, highlighting cilia as a promising senotherapy target.
- Xiaoyu Ma
- , Yingyi Zhang
- & Jinghua Hu
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Article
| Open AccessCiliary transition zone proteins coordinate ciliary protein composition and ectosome shedding
Cilia project from cells to serve sensory functions, and ciliary disruption can result in multiple disorders known as ciliopathies. Here the authors show that the ciliopathy gene TCTN1 functions to regulate the ciliary transition zone and ectosome formation.
- Liang Wang
- , Xin Wen
- & Muqing Cao
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Article
| Open AccessMicroridge-like structures anchor motile cilia
Motile cilia beat in a defined direction to orchestrate developmental programs, but also to execute janitorial tasks such as clearing airways. Here they show that motile cilia of the Xenopus epidermis are anchored to microridge-like membrane protrusions to maintain their directionality.
- Takayuki Yasunaga
- , Johannes Wiegel
- & Gerd Walz
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Article
| Open AccessPrimary cilia on muscle stem cells are critical to maintain regenerative capacity and are lost during aging
Repair of muscle damage requires muscle stem cells, which lose regenerative capacity with aging. Here, the authors show that a sensory organelle, the primary cilium, is critical for muscle stem cell proliferation during regeneration and lost with aging.
- Adelaida R. Palla
- , Keren I. Hilgendorf
- & Helen M. Blau
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Article
| Open AccessLoss of the Bardet-Biedl protein Bbs1 alters photoreceptor outer segment protein and lipid composition
Primary cilia are key sensory organelles whose dysfunction leads to ciliopathy disorders such as Bardet-Biedl syndrome (BBS). Here they identify a role for Bbs1 in lipid homeostasis of photoreceptor outer segments in zebrafish, which may contribute to vision loss in patients with Bardet-Biedl syndrome.
- Markus Masek
- , Christelle Etard
- & Ruxandra Bachmann-Gagescu
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Article
| Open AccessCilia locally synthesize proteins to sustain their ultrastructure and functions
Cilia are microtubule-based organelles containing proteins transported from the cell body. Here, the authors show that the multicilia of mouse ependymal cells contain ribosomal components, tubulin mRNA,18 S rRNA and nascent tubulin peptides, suggesting local translation in the ciliary compartment.
- Kai Hao
- , Yawen Chen
- & Xueliang Zhu
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Article
| Open AccessSub-centrosomal mapping identifies augmin-γTuRC as part of a centriole-stabilizing scaffold
The γ-tubulin ring complex (γTuRC) nucleates microtubules at the centrosome, but how this function is related to γTuRC subcentrosomal distribution is unclear. Here the authors show that γTuRC in the centriole lumen has a nucleation-independent role in centriole integrity and cilium assembly.
- Nina Schweizer
- , Laurence Haren
- & Jens Lüders
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Article
| Open AccessWdr47, Camsaps, and Katanin cooperate to generate ciliary central microtubules
Ciliary beating is mediated by the axonemal central pair microtubules, though how these non-centrosomal microtubules form is poorly understood. Here the authors show that a trio of proteins act cooperatively to initiate central microtubule formation in mammals.
- Hao Liu
- , Jianqun Zheng
- & Xiumin Yan
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Article
| Open AccessSARS-CoV-2 infection induces the dedifferentiation of multiciliated cells and impairs mucociliary clearance
SARS-CoV-2 infection damages the airways. Here the authors show that SARS-CoV-2 infection induces the rapid loss of airway motile cilia, resulting in altered cilia clearance function. Cilia loss is preceded by reduced expression of the ciliogenesis regulator Foxj1.
- Rémy Robinot
- , Mathieu Hubert
- & Lisa A. Chakrabarti
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| Open AccessNEK9 regulates primary cilia formation by acting as a selective autophagy adaptor for MYH9/myosin IIA
Ciliogenesis is a tightly regulated process, although the role of selective autophagy is unclear. Here, the authors show NIMA-related kinase 9 controls actin network stabilization and subsequently ciliogenesis by targeting myosin MYH9 for autophagic degradation via GABARAP interaction.
- Yasuhiro Yamamoto
- , Haruka Chino
- & Noboru Mizushima
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Article
| Open AccessHigh proliferation and delamination during skin epidermal stratification
How the developing skin epidermis is transformed from a simple single-layered epithelium to a complex and stratified barrier is still an open question. Here, the authors provide a model based on high proliferation and delamination of the keratinocyte progenitors that support the stratification process.
- Mareike Damen
- , Lisa Wirtz
- & Hisham Bazzi
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| Open AccessThe decrease of intraflagellar transport impairs sensory perception and metabolism in ageing
Sensory perception and metabolic homeostasis are known to deteriorate with ageing, while mechanisms underlying their deterioration remain poorly understood. Here, the authors demonstrate that decrease of intraflagellar transport in the cilia of sensory neurons impairs sensory perception and metabolism in ageing C. elegans.
- Yincong Zhang
- , Xiaona Zhang
- & Yidong Shen
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Article
| Open AccessFibrogranular materials function as organizers to ensure the fidelity of multiciliary assembly
Multicilia are complex, and how they achieve accurate assembly is unclear. Here, the authors show that fibrogranular materials condense into spherical cores and function in multicilia formation by tightly associating with deuterosomes and concentrating specific proteins to promote proper assembly.
- Huijie Zhao
- , Qingxia Chen
- & Xueliang Zhu
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Article
| Open AccessStructure of a microtubule-bound axonemal dynein
Axonemal dyneins are tethered to doublet microtubules inside cilia to drive ciliary beating but the mechanisms regulating their localization and function are poorly understood. Here authors report a cryo-EM reconstruction of a three-headed axonemal dynein natively bound to doublet microtubules isolated from cilia which provides a framework to understand the roles of individual subunits.
- Travis Walton
- , Hao Wu
- & Alan Brown
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Article
| Open AccessTALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating
Most cells possess sensory cilia, which need to be gated properly. Here the authors show that the C. elegans proteins TALP-3 and ANKR-26 coordinate cilia gating in the context of transition fibers and that this mechanism is conserved in mammalian cells and likely implicated in certain ciliopathies.
- Hao Yan
- , Chuan Chen
- & Qing Wei
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Article
| Open AccessThe dynamic and structural properties of axonemal tubulins support the high length stability of cilia
The axoneme in cilia and flagella has exceptionally high stability despite being composed of microtubules that are known to be highly dynamic. Here authors extract tubulin from different components of Chlamydomonas reinhardtii axonemes and characterize their properties.
- Ron Orbach
- & Jonathon Howard
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| Open AccessInvestigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport
PACSIN proteins are known to mediate membrane tubulation. Here the authors show that PACSIN - positive membranous tubules extend from the ciliary pocket membrane and during ciliogenesis from the ciliary vesicle to the plasma membrane, called extracellular membrane channels (EMCs).
- Christine Insinna
- , Quanlong Lu
- & Christopher J. Westlake
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| Open AccessMicrotubule asters anchored by FSD1 control axoneme assembly and ciliogenesis
Microtubule asters originate from centrosomes but their role during interphase remains largely unknown. Here, the authors find that microtubule asters anchored by previously-uncharacterized FSD1 play a role in ciliogenesis by maintaining the dynamic localization of centriolar satellites.
- Hai-Qing Tu
- , Xuan-He Qin
- & Hui-Yan Li
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Article
| Open AccessM-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole
Ciliogenesis is negatively regulated by the CP110-CEP97 complex, although the mechanism controlling mother centriole localization is poorly understood. Here, Huang et al. show that KIF24 recruits MMP9 to the mother centriole, where it regulates ciliogenesis by controlling CP110-CEP97 recruitment.
- Ning Huang
- , Donghui Zhang
- & Jianguo Chen
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Article
| Open AccessEpendymal cilia beating induces an actin network to protect centrioles against shear stress
Ependymal ciliary beating contributes to the flow of cerebrospinal fluid in the brain ventricles and these cilia resist the flow forces. Here the authors show that the assembly of a dense actin network around the centrioles is induced by cilia beating to protect centrioles against the shear stress generated by ciliary motility.
- Alexia Mahuzier
- , Asm Shihavuddin
- & Nathalie Delgehyr
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| Open AccessSuper-resolution architecture of mammalian centriole distal appendages reveals distinct blade and matrix functional components
Distal appendages (DAPs) at the cilia base mediate membrane docking during ciliogenesis. Here the authors use super-resolution microscopy to map 16 centriole distal end components, revealing the structure of the backbone of the DAP, as well as a previously undescribed distal appendage matrix.
- T. Tony Yang
- , Weng Man Chong
- & Jung-Chi Liao
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Article
| Open AccessSpatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling
Tubulin post-translational modifications (PTMs) occur spatiotemporally throughout cells, therefore assessing the physiological roles in specific subcellular compartments has been challenging. Here the authors develop a method to rapidly deplete tubulin glutamylation inside the primary cilia by targeting an engineered deglutamylase to the axoneme.
- Shi-Rong Hong
- , Cuei-Ling Wang
- & Yu-Chun Lin
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| Open AccessThe CPLANE protein Intu protects kidneys from ischemia-reperfusion injury by targeting STAT1 for degradation
Intu is a planar cell polarity protein known to regulate ciliogenesis during embryonic development. Here, Wang et al. identify a role for Intu in adult kidneys, where they find it promotes degradation of STAT1 and thus prevents cilia loss and cell death upon ischemia-reperfusion injury.
- Shixuan Wang
- , Aimin Liu
- & Zheng Dong
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Article
| Open AccessEGF receptor kinase suppresses ciliogenesis through activation of USP8 deubiquitinase
The trichoplein-Aurora A pathway inhibits ciliogenesis in proliferating cells. Here the authors EGFR-mediated phosphorylation of the deubiquitinating enzyme USP8 leads to its activation, and this suppresses trichoplein degradation, allowing inhibition of ciliogenesis.
- Kousuke Kasahara
- , Hiromasa Aoki
- & Masaki Inagaki
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| Open AccessMutations in CFAP43 and CFAP44 cause male infertility and flagellum defects in Trypanosoma and human
Asthenozoospermia is a major cause of male infertility, and multiple morphological abnormalities of the flagella (MMAF) is a particularly severe form. Here, using whole-exome sequencing of 78 MMAF patients, the authors identify mutations in two WDR proteins, CFAP43 and CFAP44, and confirm that these proteins are required for flagellogenesis in mouse and Trypanosoma brucei.
- Charles Coutton
- , Alexandra S. Vargas
- & Pierre F. Ray
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| Open AccessCytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis
Multiciliogenesis requires activation of transcriptional and protein assembly programs; however, the mechanisms that initiate the formation of these multiprotein complexes are unclear. Here the authors show that after inducing centriole biogenesis genes, the transcription factor E2f4 is required in the cytoplasm for assembly and nucleation of deuterosomes.
- Munemasa Mori
- , Renin Hazan
- & Wellington V. Cardoso
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Article
| Open AccessSubnanometre-resolution structure of the doublet microtubule reveals new classes of microtubule-associated proteins
Cilia are hair-like appendages involved in cell motility and sensory reception. Here, the authors report a high resolution cryo-EM structure of the microtubule doublet from motile cilia and identify microtubule inner proteins (MIPs) bound to the inner surface of the doublet that appear to stabilize its structure.
- Muneyoshi Ichikawa
- , Dinan Liu
- & Khanh Huy Bui
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Article
| Open AccessCell-free reconstitution reveals centriole cartwheel assembly mechanisms
The centriole is an organelle composed of rings of SAS-6 proteins that form a cartwheel structure. Here the authors develop a cell-free system to examine core cartwheel assembly ofC. reinhardtiiproteins and discover that CrSAS-6 has autonomous properties that facilitates self-organized stacking of pairs of rings.
- P. Guichard
- , V. Hamel
- & P. Gönczy
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Article
| Open AccessEnsemble and single-molecule dynamics of IFT dynein in Caenorhabditis elegans cilia
Cytoplasmic dynein 2 drives retrograde intraflagellar transport but little is known about its dynamics. Here the authors use fluorescence microscopy to track labelled dynein 2 inC. elegansat the single-molecule level and report diffusion at the ciliary base, and pausing and directional switches along the cilium.
- Jona Mijalkovic
- , Bram Prevo
- & Erwin J. G. Peterman
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Article
| Open AccessMolecular basis for CPAP-tubulin interaction in controlling centriolar and ciliary length
Centrioles and cilia are microtubule-based structures of defined architecture, but what regulates this architecture is not clear. Here the authors discover that centrosomal-P4.1-associated-protein (CPAP) binds the α/β-tubulin dimer and licenses it for tubulin delivery contributing to centriolar/ciliary length and architecture control.
- Xiangdong Zheng
- , Anand Ramani
- & Haitao Li
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Article
| Open AccessPDE6δ-mediated sorting of INPP5E into the cilium is determined by cargo-carrier affinity
PDE6δ regulates the sorting of prenylated cargo proteins. Here Fansa et al. propose that the affinity of the interaction between PDE6δ and its cargo protein determines whether they are released by cytoplasmic or cilia-specific release factors ultimately determining their subcellular localization.
- Eyad Kalawy Fansa
- , Stefanie Kristine Kösling
- & Shehab Ismail
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Article
| Open AccessBasal body multipotency and axonemal remodelling are two pathways to a 9+0 flagellum
Whether basal bodies are pre-committed to form 9+2 motile or 9+0 sensory axonemes and whether interconversion occurs between the two types of axonemes is not clear. Here, the authors used the unicellular eukaryote Leishmania as a model system to demonstrate that 9+0 axonemes can be formed de novoor by restructuring of 9+2 axonemes.
- R. J. Wheeler
- , E. Gluenz
- & K. Gull
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Article
| Open AccessPTEN regulates cilia through Dishevelled
The formation of motile cilia is regulated by Dishevelled (DVL), a central component in WNT signalling and planar cell polarity (PCP). Here the authors identify DVL as a novel substrate of the phosphatase PTEN, coupling PTEN to cilia dynamics and PCP.
- Iryna Shnitsar
- , Mikhail Bashkurov
- & Miriam Barrios-Rodiles
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Article
| Open AccessNek2 activation of Kif24 ensures cilium disassembly during the cell cycle
Most differentiated mammalian cells assemble a primary cilium, which serves as a cellular ‘antenna’ for sensing and responding to the extracellular environment. Here the authors show that Nek2-mediated phosphorylation of Kif24 further promotes the loss of primary cilia, triggered by Aurora A and HDAC6 on cell cycle re-entry.
- Sehyun Kim
- , Kwanwoo Lee
- & Brian David Dynlacht
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Mutations in DYNC2LI1 disrupt cilia function and cause short rib polydactyly syndrome
Mutations in genes affecting intraflagellar transport account for some but not all cases of short rib polydactyly syndromes. Here Taylor et al. use whole exome sequencing and in vivo cell line assays to identify novel disease associated mutations in DYNC2LI1.
- S. Paige Taylor
- , Tiago J. Dantas
- & Deborah Krakow
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ERK7 regulates ciliogenesis by phosphorylating the actin regulator CapZIP in cooperation with Dishevelled
The actin regulator CapZIP has been shown to regulate ciliogenesis in a phosphorylation-dependent manner. Miyatake et al.show that the atypical MAP kinase ERK7 promotes ciliogenesis by phophorylating CapZIP, and that interactions between both proteins and Dishevelled are required for CapZIP phosphorylation.
- Koichi Miyatake
- , Morioh Kusakabe
- & Eisuke Nishida
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Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue
The ciliary margin of the eye functions as a source of multipotent progenitor cells in certain organisms but whether it plays this role in humans has not been easy to study. Here the authors culture human embryonic stem cells that self-organize into retinal tissue, and show that ciliary margin-like growth zones emerge from the developing human retinal tissue and contain stem cell niches.
- Atsushi Kuwahara
- , Chikafumi Ozone
- & Yoshiki Sasai
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ANKS6 is the critical activator of NEK8 kinase in embryonic situs determination and organ patterning
Protein kinase NEK8 is important for cilliary function, but the mechanism by which it acts is unknown. Czarnecki et al. identify the cilliary protein ANKS6 as a target and crucial activator of NEK8 and describe the importance of this protein interaction in embryonic development and organogenesis.
- Peter G. Czarnecki
- , George C. Gabriel
- & Jagesh V. Shah
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Article
| Open AccessDirect evidence for BBSome-associated intraflagellar transport reveals distinct properties of native mammalian cilia
Loss of olfactory function is one of the many symptoms of the ciliopathy Bardet–Biedl syndrome. Williams et al. show that Bardet–Biedl proteins are components of intraflagellar transport particles within cilia, and directly visualize their trafficking in native mammalian olfactory neurons.
- Corey L. Williams
- , Jeremy C. McIntyre
- & Jeffrey R. Martens
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Cryo-electron tomography reveals ciliary defects underlying human RSPH1 primary ciliary dyskinesia
Our current understanding of cilia biology and ciliary diseases is incomplete, in part because cilia are hard to visualize. Here, the authors use cryo-electron tomography to image the structure of human cilia with high resolution and uncover the elusive ciliary defects in Primary Ciliary Dyskinesia patients.
- Jianfeng Lin
- , Weining Yin
- & Daniela Nicastro
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Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents
Cilia are hair-like protuberances on the cellular surface that have been implicated in sensing and signal transduction. Here Gerdes et al. show cilia are involved in insulin signalling and secretion in pancreatic β-cells of rodents, and suggest that ciliary dysfunction could contribute to type 2 diabetes.
- Jantje M. Gerdes
- , Sonia Christou-Savina
- & Per-Olof Berggren